ID   SIRB_PRIMG              Reviewed;         266 AA.
AC   P61817;
DT   07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2004, sequence version 1.
DT   03-AUG-2022, entry version 51.
DE   RecName: Full=Sirohydrochlorin ferrochelatase;
DE            EC=4.99.1.4;
GN   Name=sirB;
OS   Priestia megaterium (Bacillus megaterium).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Priestia.
OX   NCBI_TaxID=1404;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 509 / CCM 1464 / NBRC 12109;
RX   PubMed=12408752; DOI=10.1042/bj20021443;
RA   Raux E., Leech H.K., Beck R., Schubert H.L., Santander P.J., Roessner C.A.,
RA   Scott A.I., Martens J.H., Jahn D., Thermes C., Rambach A., Warren M.J.;
RT   "Identification and functional analysis of enzymes required for precorrin-2
RT   dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and
RT   cobalamin in Bacillus megaterium.";
RL   Biochem. J. 370:505-516(2003).
CC   -!- FUNCTION: Chelates iron to the siroheme precursor.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + siroheme = Fe(2+) + sirohydrochlorin;
CC         Xref=Rhea:RHEA:24360, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033,
CC         ChEBI:CHEBI:58351, ChEBI:CHEBI:60052; EC=4.99.1.4;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme
CC       biosynthesis; siroheme from sirohydrochlorin: step 1/1.
CC   -!- SIMILARITY: Belongs to the CbiX family. SirB subfamily. {ECO:0000305}.
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DR   EMBL; AJ509159; CAD48922.1; -; Genomic_DNA.
DR   AlphaFoldDB; P61817; -.
DR   SMR; P61817; -.
DR   PRIDE; P61817; -.
DR   BioCyc; MetaCyc:MON-18854; -.
DR   UniPathway; UPA00262; UER00376.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051266; F:sirohydrochlorin ferrochelatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019354; P:siroheme biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR002762; CbiX-like.
DR   Pfam; PF01903; CbiX; 2.
PE   3: Inferred from homology;
KW   Iron; Lyase; Metal-binding; Porphyrin biosynthesis.
FT   CHAIN           1..266
FT                   /note="Sirohydrochlorin ferrochelatase"
FT                   /id="PRO_0000150365"
FT   BINDING         20
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         86
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   266 AA;  29938 MW;  3AF66D78590727B7 CRC64;
     MHKKLTKEVD YMDAVLYVCH GSRVKEGADQ AVAFIERCKK NLDVPIQEVC FLELASPTIE
     QGFEACIEQG ATRIAIVPLL LLTAAHAKHD IPEEIQKVYE RYPQVEVLYG EPFGVDERIV
     DILVERINET NVDKHEDSMV LLVGRGSSDP AVKRDLNEIA QLLKGKGAFK EVSTCYLAAA
     SPNLKEGLHL AKRTSYKQVF VLPYLLFTGI LMNEIKEELE QLSTDAQQFI LANYLGYHDG
     LAHILSHQVK TLLSSKGNQY DVYRYA