SIRP1_ORYSJ
ID SIRP1_ORYSJ Reviewed; 323 AA.
AC Q6AVN2; Q0DH72;
DT 07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=E3 ubiquitin-protein ligase SIRP1 {ECO:0000305};
DE EC=2.3.2.27 {ECO:0000269|PubMed:27118216};
DE AltName: Full=Salt-induced RING finger protein 1 {ECO:0000303|PubMed:27118216};
DE Short=OsSIRP1 {ECO:0000303|PubMed:27118216};
GN Name=SIRP1 {ECO:0000303|PubMed:27118216};
GN OrderedLocusNames=Os05g0488800 {ECO:0000312|EMBL:BAS94636.1},
GN LOC_Os05g40980 {ECO:0000305};
GN ORFNames=OJ1119_H02.11 {ECO:0000312|EMBL:AAT77283.1},
GN OsJ_19008 {ECO:0000312|EMBL:EEE64176.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RG The rice full-length cDNA consortium;
RT "Oryza sativa full length cDNA.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, INDUCTION, AND
RP MUTAGENESIS OF CYS-225.
RX PubMed=27118216; DOI=10.1111/ppl.12459;
RA Hwang S.G., Kim J.J., Lim S.D., Park Y.C., Moon J.C., Jang C.S.;
RT "Molecular dissection of Oryza sativa salt-induced RING Finger Protein 1
RT (OsSIRP1): possible involvement in the sensitivity response to salinity
RT stress.";
RL Physiol. Plantarum 158:168-179(2016).
CC -!- FUNCTION: Possesses E3 ubiqutin-protein ligase activity in vitro. Acts
CC as negative regulator of salinity stress tolerance mediated by the
CC ubiquitin-proteasome degradation pathway.
CC {ECO:0000269|PubMed:27118216}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000269|PubMed:27118216};
CC -!- PATHWAY: Protein modification; protein ubiquitination. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:27118216}.
CC -!- INDUCTION: Induced by salt stress in roots (PubMed:27118216). Induced
CC by heat shock, drought stress and abscisic acid (ABA)
CC (PubMed:27118216). {ECO:0000269|PubMed:27118216}.
CC -!- DOMAIN: The RING-type zinc finger domain mediates binding to an E2
CC ubiquitin-conjugating enzyme. {ECO:0000250}.
CC -!- MISCELLANEOUS: Plants over-expressing SIRP1 exhibit enhanced
CC sensitivity to seed germination and root growth inhibition by salt
CC stress. {ECO:0000269|PubMed:27118216}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF17801.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC097175; AAT77283.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF17801.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014961; BAS94636.1; -; Genomic_DNA.
DR EMBL; CM000142; EEE64176.1; -; Genomic_DNA.
DR EMBL; AK240640; BAH00840.1; -; mRNA.
DR RefSeq; XP_015637458.1; XM_015781972.1.
DR AlphaFoldDB; Q6AVN2; -.
DR SMR; Q6AVN2; -.
DR STRING; 4530.OS05T0488800-01; -.
DR PaxDb; Q6AVN2; -.
DR PRIDE; Q6AVN2; -.
DR EnsemblPlants; Os05t0488800-01; Os05t0488800-01; Os05g0488800.
DR GeneID; 4339152; -.
DR Gramene; Os05t0488800-01; Os05t0488800-01; Os05g0488800.
DR KEGG; osa:4339152; -.
DR eggNOG; KOG0800; Eukaryota.
DR HOGENOM; CLU_034892_4_0_1; -.
DR InParanoid; Q6AVN2; -.
DR OMA; DMDSHIQ; -.
DR OrthoDB; 1249953at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000007752; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR039525; RNF126-like_zinc-ribbon.
DR InterPro; IPR029040; RPABC4/Spt4.
DR InterPro; IPR039738; SIRP1.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR22765:SF261; PTHR22765:SF261; 1.
DR Pfam; PF13639; zf-RING_2; 1.
DR Pfam; PF14369; zinc_ribbon_9; 1.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF63393; SSF63393; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Metal-binding; Reference proteome; Stress response; Transferase;
KW Ubl conjugation pathway; Zinc; Zinc-finger.
FT CHAIN 1..323
FT /note="E3 ubiquitin-protein ligase SIRP1"
FT /id="PRO_0000440260"
FT ZN_FING 199..240
FT /note="RING-type; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 248..280
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 296..323
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 248..271
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 225
FT /note="C->A: Loss of catalytic activity."
FT /evidence="ECO:0000269|PubMed:27118216"
SQ SEQUENCE 323 AA; 35562 MW; EDD8C2C3BC814DAF CRC64;
MAEAAITRYW CHECEQAIEE AMVDEIKCPS CGGGFVEEMT DEEIERLTNR QPEPGFSQWN
PIEHPGETMD SDDEDNDLGR EFEGFIRRHR RASTLRRVLD SIHDDLADDQ ERDSSILINA
FNQALALQGS VLDPDEGQGD QGGSTNDDGL LEEYVLGAGL SLLLQHLAES DPSRNGTPPA
KKEAVEALPT VKIEEVVSCS VCLDDLEVGS QAKQMPCEHK FHSSCILPWL ELHSSCPVCR
FELPSEETKD LNEPSNIGRV EDSHEEVRAD GPGNVSESSN RPWAIVPWLN ELFSTREAQN
AGGVSTDQQS PHTSGTNPNA GHS
