SIT1_YEAST
ID SIT1_YEAST Reviewed; 628 AA.
AC P39980; D3DLI5; E9P951;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Siderophore iron transporter 1;
DE AltName: Full=Ferrioxamine B permease;
DE AltName: Full=Siderophore iron transporter ARN3;
GN Name=SIT1; Synonyms=ARN3; OrderedLocusNames=YEL065W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RX PubMed=10744769; DOI=10.1074/jbc.275.14.10709;
RA Yun C.-W., Ferea T., Rashford J., Ardon O., Brown P.O., Botstein D.,
RA Kaplan J., Philpott C.C.;
RT "Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence
RT for two pathways of iron uptake.";
RL J. Biol. Chem. 275:10709-10715(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP FUNCTION.
RX PubMed=9884238; DOI=10.1099/00221287-144-12-3455;
RA Lesuisse E., Simon-Casteras M., Labbe P.;
RT "Siderophore-mediated iron uptake in Saccharomyces cerevisiae: the SIT1
RT gene encodes a ferrioxamine B permease that belongs to the major
RT facilitator superfamily.";
RL Microbiology 144:3455-3462(1998).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=10748025; DOI=10.1074/jbc.m001456200;
RA Yun C.-W., Tiedeman J.S., Moore R.E., Philpott C.C.;
RT "Siderophore-iron uptake in Saccharomyces cerevisiae. Identification of
RT ferrichrome and fusarinine transporters.";
RL J. Biol. Chem. 275:16354-16359(2000).
CC -!- FUNCTION: Involved in the transport of siderophore ferrioxamine B and
CC so has a role in iron homeostasis. {ECO:0000269|PubMed:9884238}.
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000269|PubMed:10744769,
CC ECO:0000269|PubMed:10748025}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:10744769, ECO:0000269|PubMed:10748025}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; U18795; AAB65022.1; -; Genomic_DNA.
DR EMBL; AY723800; AAU09717.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07589.1; -; Genomic_DNA.
DR PIR; S50524; S50524.
DR RefSeq; NP_010849.3; NM_001178880.3.
DR AlphaFoldDB; P39980; -.
DR BioGRID; 36664; 50.
DR DIP; DIP-7273N; -.
DR STRING; 4932.YEL065W; -.
DR TCDB; 2.A.1.16.1; the major facilitator superfamily (mfs).
DR iPTMnet; P39980; -.
DR MaxQB; P39980; -.
DR PaxDb; P39980; -.
DR PRIDE; P39980; -.
DR EnsemblFungi; YEL065W_mRNA; YEL065W; YEL065W.
DR GeneID; 856644; -.
DR KEGG; sce:YEL065W; -.
DR SGD; S000000791; SIT1.
DR VEuPathDB; FungiDB:YEL065W; -.
DR eggNOG; KOG0254; Eukaryota.
DR GeneTree; ENSGT00940000176305; -.
DR HOGENOM; CLU_012970_2_1_1; -.
DR InParanoid; P39980; -.
DR OMA; VMLNTAW; -.
DR BioCyc; YEAST:G3O-30180-MON; -.
DR PRO; PR:P39980; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P39980; protein.
DR GO; GO:0031410; C:cytoplasmic vesicle; IDA:SGD.
DR GO; GO:0005768; C:endosome; IDA:SGD.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005774; C:vacuolar membrane; IBA:GO_Central.
DR GO; GO:0015343; F:siderophore transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015344; F:siderophore uptake transmembrane transporter activity; IMP:SGD.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006879; P:cellular iron ion homeostasis; IDA:SGD.
DR GO; GO:0015891; P:siderophore transport; IMP:SGD.
DR GO; GO:0055085; P:transmembrane transport; IMP:SGD.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR036259; MFS_trans_sf.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 3: Inferred from homology;
KW Endosome; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..628
FT /note="Siderophore iron transporter 1"
FT /id="PRO_0000084878"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 394..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 420..440
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 448..468
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 488..508
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 559..579
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 386
FT /note="E -> G (in Ref. 4; AAU09717)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 628 AA; 70562 MW; 39E711CF861CEF05 CRC64;
MDPGIANHTL PEEFEEVVVP EMLEKEVGAK VDVKPTLTTS SPAPSYIELI DPGVHNIEIY
AEMYNRPIYR VALFFSLFLI AYAYGLDGNI RYTFQAYATS SYSQHSLLST VNCIKTVIAA
VGQIFFARLS DIFGRFSIMI VSIIFYSMGT IIESQAVNIT RFAVGGCFYQ LGLTGIILIL
EVIASDFSNL NWRLLALFIP ALPFIINTWI SGNVTSAIDA NWKWGIGMWA FILPLACIPL
GICMLHMRYL ARKHAKDRLK PEFEALNKLK WKSFCIDIAF WKLDIIGMLL ITVFFGCVLV
PFTLAGGLKE EWKTAHIIVP EVIGWVVVLP LYMLWEIKYS RHPLTPWDLI QDRGIFFALL
IAFFINFNWY MQGDYMYTVL VVAVHESIKS ATRITSLYSF VSVIVGTILG FILIKVRRTK
PFIIFGISCW IVSFGLLVHY RGDSGAHSGI IGSLCLLGFG AGSFTYVTQA SIQASAKTHA
RMAVVTSLYL ATYNIGSAFG SSVSGAVWTN ILPKEISKRI SDPTLAAQAY GSPFTFITTY
TWGTPERIAL VMSYRYVQKI LCIIGLVFCF PLLGCAFMLR NHKLTDSIAL EGNDHLESKN
TFEIEEKEES FLKNKFFTHF TSSKDRKD
