ID   SIT2_ASPFU              Reviewed;         607 AA.
AC   Q4WGE2;
DT   20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Siderochrome iron transporter 2 {ECO:0000303|PubMed:26929401};
GN   Name=sit2 {ECO:0000303|PubMed:26929401}; ORFNames=AFUA_7G04730;
OS   Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS   A1100) (Aspergillus fumigatus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=330879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX   PubMed=16372009; DOI=10.1038/nature04332;
RA   Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA   Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA   Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA   Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA   Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA   Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA   Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA   Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA   Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA   Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA   O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA   Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA   Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA   Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA   Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA   Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA   Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA   Barrell B.G., Denning D.W.;
RT   "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT   Aspergillus fumigatus.";
RL   Nature 438:1151-1156(2005).
RN   [2]
RP   FUNCTION, INDUCTION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES,
RP   AND DISRUPTION PHENOTYPE.
RX   PubMed=26929401; DOI=10.1042/bcj20160066;
RA   Park Y.S., Kim J.Y., Yun C.W.;
RT   "Identification of ferrichrome- and ferrioxamine B-mediated iron uptake by
RT   Aspergillus fumigatus.";
RL   Biochem. J. 473:1203-1213(2016).
CC   -!- FUNCTION: Major facilitator transporter involved in ferrichrome (FC)
CC       uptake (PubMed:26929401). {ECO:0000269|PubMed:26929401}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.26 uM for ferrichrome (FC) uptake {ECO:0000269|PubMed:26929401};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:26929401};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- INDUCTION: Highly expressed under low-iron conditions and down-
CC       regulated under high-iron conditions (PubMed:26929401). Expression is
CC       regulated by hapX under low-iron conditions (PubMed:26929401).
CC       {ECO:0000269|PubMed:26929401}.
CC   -!- DISRUPTION PHENOTYPE: Leads to lower ferrichrome (FC) uptake activity,
CC       which is reduced to approximately 60% of the wild-type activity
CC       (PubMed:26929401). {ECO:0000269|PubMed:26929401}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
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DR   EMBL; AAHF01000009; EAL86999.1; -; Genomic_DNA.
DR   RefSeq; XP_749037.1; XM_743944.1.
DR   AlphaFoldDB; Q4WGE2; -.
DR   SMR; Q4WGE2; -.
DR   STRING; 746128.CADAFUBP00008776; -.
DR   EnsemblFungi; EAL86999; EAL86999; AFUA_7G04730.
DR   GeneID; 3506290; -.
DR   KEGG; afm:AFUA_7G04730; -.
DR   VEuPathDB; FungiDB:Afu7g04730; -.
DR   eggNOG; KOG0254; Eukaryota.
DR   HOGENOM; CLU_012970_1_0_1; -.
DR   InParanoid; Q4WGE2; -.
DR   OMA; AVWKNYR; -.
DR   OrthoDB; 641071at2759; -.
DR   Proteomes; UP000002530; Chromosome 7.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Glycoprotein; Ion transport; Iron; Iron transport; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..607
FT                   /note="Siderochrome iron transporter 2"
FT                   /id="PRO_0000444406"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        129..149
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        180..200
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        210..230
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        242..262
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        326..346
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        367..387
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        404..424
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        432..452
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        459..479
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        499..519
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        573..593
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..46
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        538
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   607 AA;  66599 MW;  7A7BCE9694860AB2 CRC64;
     MGLFGSFGAR NKATPQVPPG AVAKAPEGTP KGPETNDQPD MDSSRLSLEA RNEKEIEQHP
     NQVTADAHLG VQKVEAAALV WSKKAVWATY AWIWVCFFLL ALQSGISGNV INNAYSGFMS
     APQISTANIL SSIIGGVLKL PIAKILNLWG RAEGFLVFVG VYTIGLIILA ACNGPDSYAA
     GYVLFWIGYD AIYLILDVFV ADTSGLRNRA FTFAFASTPF ICTAFTAPLA ASSFLRMTTW
     RWAYGAFAII MPVALAPLAV VFKRYQLKAE KMGLYVRQPS GRTWAQSVAH YIHEFDIIGA
     FLLMAAWVLL LLPFSLASNG RAEYKSAAFI AMVIIGFCLF FAFAAWEKWF ARVHFINYEL
     LKQRTVLGAC VMAATLYFSF YCWDLYFYNF CTVVYNLNAA MTGYMTQIYN VGSCFWGVVF
     GLWVRWTKHF KHTCLFFGLP LMILGAGLMI HFRGQSDDIG YVIMCQIFIA FGGGTLVIGE
     QMAVMASANR EGVPMMLSFI GLFSSLGGAI GYAVAAAIYT NVFPGALESR LPADLKSNAT
     SIYMGGYTVQ QTYPMGSAAR DAINYAWGRS QRFGAVAATC ILILGIPAIA VWKNYNVNKH
     QNKGVMI