ID   SIXI_MESMA              Reviewed;          85 AA.
AC   Q8I0K7;
DT   15-DEC-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Depressant scorpion toxin BmKIM;
DE   Flags: Precursor;
GN   Name=KIM2;
OS   Mesobuthus martensii (Manchurian scorpion) (Buthus martensii).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Mesobuthus.
OX   NCBI_TaxID=34649;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TOXIC DOSE, AND SYNTHESIS OF
RP   22-82.
RX   PubMed=12230558; DOI=10.1046/j.1432-1033.2002.03136.x;
RA   Peng F., Zeng X.-C., He X.-H., Pu J., Li W.-X., Zhu Z.-H., Liu H.;
RT   "Molecular cloning and functional expression of a gene encoding an
RT   antiarrhythmia peptide derived from the scorpion toxin.";
RL   Eur. J. Biochem. 269:4468-4475(2002).
CC   -!- FUNCTION: Causes a slow progressive depressant flaccid paralysis, when
CC       injected into S.falculata blowfly larvae. Inhibits dose-dependently the
CC       total sodium (Nav) currents both in dorsal root ganglia neurons and in
CC       ventricular myocytes. Is toxic to mice by intravenous injection, but
CC       not by subcutaneous or intracerebroventricular injection. Produces
CC       antiarrhythmia in rat. Is then active on both mammals and insects.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC       antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC       {ECO:0000305}.
CC   -!- TOXIC DOSE: LD(50) is 0.8 mg/kg by intravenous injection into mice.
CC       {ECO:0000269|PubMed:12230558}.
CC   -!- SIMILARITY: Belongs to the long (4 C-C) scorpion toxin superfamily.
CC       Sodium channel inhibitor family. {ECO:0000305}.
CC   -!- CAUTION: PubMed:12230558 uses only BmKIM as the name, whereas the
CC       authors submitted sequences using KIM2 as the name. {ECO:0000305}.
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DR   EMBL; AF459791; AAN59782.1; -; Genomic_DNA.
DR   EMBL; AF459792; AAN59783.1; -; mRNA.
DR   AlphaFoldDB; Q8I0K7; -.
DR   SMR; Q8I0K7; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:InterPro.
DR   CDD; cd00107; Knot1; 1.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR044062; LCN-type_CS_alpha_beta_dom.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   InterPro; IPR018218; Scorpion_toxinL.
DR   InterPro; IPR002061; Scorpion_toxinL/defensin.
DR   Pfam; PF00537; Toxin_3; 1.
DR   PRINTS; PR00285; SCORPNTOXIN.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS51863; LCN_CSAB; 1.
PE   2: Evidence at transcript level;
KW   Amidation; Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW   Secreted; Signal; Toxin; Voltage-gated sodium channel impairing toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..82
FT                   /note="Depressant scorpion toxin BmKIM"
FT                   /id="PRO_0000035210"
FT   DOMAIN          22..82
FT                   /note="LCN-type CS-alpha/beta"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   MOD_RES         82
FT                   /note="Glycine amide"
FT                   /evidence="ECO:0000250"
FT   DISULFID        31..81
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        35..56
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        42..63
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        46..65
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
SQ   SEQUENCE   85 AA;  9425 MW;  5076039B9966AA6F CRC64;
     MKLFLLLVFF ASMLIDGLVN ADGYIRGSNG CKISCLWGNE GCNKECKGFG AYYGYCWTWG
     LACWCEGLPD DKTWKSESNT CGGKK