ID   SIZ2_ORYSJ              Reviewed;         813 AA.
AC   Q6ASW7; A0A0P0W394;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=E3 SUMO-protein ligase SIZ2;
DE            EC=2.3.2.-;
DE   AltName: Full=E3 SUMO-protein transferase SIZ2 {ECO:0000305};
GN   Name=SIZ2; OrderedLocusNames=Os03g0719100, LOC_Os03g50980;
GN   ORFNames=B1377B10.3, OsJ_12369;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Probable SUMO E3 ligase that may regulate Pi starvation
CC       responses. {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein sumoylation.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PIAS family. {ECO:0000305}.
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DR   EMBL; AC147426; AAT77838.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF98573.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF13006.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS86104.1; -; Genomic_DNA.
DR   EMBL; CM000140; EEE59819.1; -; Genomic_DNA.
DR   EMBL; AK065127; BAG89374.1; -; mRNA.
DR   RefSeq; XP_015632175.1; XM_015776689.1.
DR   AlphaFoldDB; Q6ASW7; -.
DR   SMR; Q6ASW7; -.
DR   STRING; 4530.OS03T0719100-01; -.
DR   PaxDb; Q6ASW7; -.
DR   PRIDE; Q6ASW7; -.
DR   EnsemblPlants; Os03t0719100-01; Os03t0719100-01; Os03g0719100.
DR   GeneID; 4333925; -.
DR   Gramene; Os03t0719100-01; Os03t0719100-01; Os03g0719100.
DR   KEGG; osa:4333925; -.
DR   eggNOG; KOG2169; Eukaryota.
DR   HOGENOM; CLU_015870_0_0_1; -.
DR   InParanoid; Q6ASW7; -.
DR   OMA; INMSIVK; -.
DR   OrthoDB; 141085at2759; -.
DR   PlantReactome; R-OSA-8879007; Response to cold temperature.
DR   UniPathway; UPA00886; -.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000007752; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q6ASW7; OS.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0061665; F:SUMO ligase activity; IDA:CACAO.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0016925; P:protein sumoylation; IDA:CACAO.
DR   Gene3D; 1.10.720.30; -; 1.
DR   Gene3D; 3.30.40.10; -; 2.
DR   InterPro; IPR003034; SAP_dom.
DR   InterPro; IPR036361; SAP_dom_sf.
DR   InterPro; IPR019786; Zinc_finger_PHD-type_CS.
DR   InterPro; IPR011011; Znf_FYVE_PHD.
DR   InterPro; IPR004181; Znf_MIZ.
DR   InterPro; IPR001965; Znf_PHD.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   Pfam; PF02037; SAP; 1.
DR   Pfam; PF02891; zf-MIZ; 1.
DR   SMART; SM00249; PHD; 1.
DR   SMART; SM00513; SAP; 1.
DR   SUPFAM; SSF57903; SSF57903; 1.
DR   SUPFAM; SSF68906; SSF68906; 1.
DR   PROSITE; PS50800; SAP; 1.
DR   PROSITE; PS01359; ZF_PHD_1; 1.
DR   PROSITE; PS51044; ZF_SP_RING; 1.
PE   2: Evidence at transcript level;
KW   Metal-binding; Nucleus; Reference proteome; Transferase;
KW   Ubl conjugation pathway; Zinc; Zinc-finger.
FT   CHAIN           1..813
FT                   /note="E3 SUMO-protein ligase SIZ2"
FT                   /id="PRO_0000383460"
FT   DOMAIN          18..52
FT                   /note="SAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00186"
FT   ZN_FING         122..179
FT                   /note="PHD-type"
FT   ZN_FING         357..440
FT                   /note="SP-RING-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00452"
FT   REGION          88..114
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          617..650
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          753..775
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        88..103
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        753..768
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         390
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00452"
FT   BINDING         392
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00452"
FT   BINDING         413
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00452"
FT   BINDING         416
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00452"
SQ   SEQUENCE   813 AA;  89547 MW;  BA6AA2BA20F04899 CRC64;
     MALDPADDPL LADCKYKLNH FRIKELKDVL HQLGLPKQGR KQELVDKIIA VLSDQQEQDS
     RLNGLPNKKM VGKETVAKIV DDTFAKMNGS TNAVPASRNQ TDSGHIVKPK RKSDDSAQLD
     VKVRCPCGYS MANDSMIKCE GPQCNTQQHV GCVIISEKPA DSVPPELPPH FYCDMCRITR
     ADPFWVTVNH PVLPVSITPC KVASDGSYAV QYFEKTFPLS RANWEMLQKD EYDLQVWCIL
     FNDSVPFRMQ WPLHSDIQIN GIPIRVVNRQ PTQQLGVNGR DDGPVLTAYV REGSNKIVLS
     RSDSRTFCLG VRIAKRRSVE QVLSLVPKEQ DGENFDNALA RVRRCVGGGT EADNADSDSD
     IEVVADSVSV NLRCPMTGSR IKIAGRFKPC VHMGCFDLEA FVELNQRSRK WQCPICLKNY
     SLDNIIIDPY FNRITALVQS CGDDVSEIDV KPDGSWRVKG GAELKGLAQW HLPDGTLCMP
     TDTRSKPNIR IVKQEIKEEP LSEETGGRLK LGIRRNNNGQ WEINKRLDSN NGQNGYIEDE
     NCVVSASNTD DENSKNGIYN PEPGQFDQLT SNIYDLDSSP MDAHFPPAPT EQDVIVLSDS
     DDDNVMVLSP GDVNFSSAHD NGNAFPPNPP EASGICGEQP RGAGPDVTSF LDGFDDLELP
     FWESSSSQDA AGTQVTDNQC EMQNFIVNHQ FLHEPILGVN LGGTAASNTL ECEHDGALQA
     CQSSDQDGDQ NQTCHDGHSG DLTNLSIIST QDSLTNGKNA SQKRTNCEDG TAGLDGSVVR
     SANGLRGEMP PLGQEQDRTV RQKLILTIES DSD