SK1_ORYSJ
ID SK1_ORYSJ Reviewed; 308 AA.
AC Q5NTH4; Q0DXJ9;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Shikimate kinase 1, chloroplastic;
DE Short=OsSK1;
DE EC=2.7.1.71;
DE Flags: Precursor;
GN Name=SK1; OrderedLocusNames=Os02g0749300, LOC_Os02g51410;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Nipponbare;
RX PubMed=15891897; DOI=10.1007/s00425-005-1559-8;
RA Kasai K., Kanno T., Akita M., Ikejiri-Kanno Y., Wakasa K., Tozawa Y.;
RT "Identification of three shikimate kinase genes in rice: characterization
RT of their differential expression during panicle development and of the
RT enzymatic activities of the encoded proteins.";
RL Planta 222:438-447(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Catalyzes the specific phosphorylation of the 3-hydroxyl
CC group of shikimic acid using ATP as a cosubstrate.
CC {ECO:0000269|PubMed:15891897}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + shikimate = 3-phosphoshikimate + ADP + H(+);
CC Xref=Rhea:RHEA:13121, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:36208, ChEBI:CHEBI:145989, ChEBI:CHEBI:456216;
CC EC=2.7.1.71; Evidence={ECO:0000269|PubMed:15891897};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250};
CC -!- PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis;
CC chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step
CC 5/7.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000305|PubMed:15891897}.
CC -!- TISSUE SPECIFICITY: Expressed in panicles.
CC {ECO:0000269|PubMed:15891897}.
CC -!- INDUCTION: By chitin oligosaccharide elicitor.
CC {ECO:0000269|PubMed:15891897}.
CC -!- SIMILARITY: Belongs to the shikimate kinase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF10039.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB188834; BAD83412.1; -; mRNA.
DR EMBL; AP005848; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP008208; BAF10039.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014958; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015626759.1; XM_015771273.1.
DR AlphaFoldDB; Q5NTH4; -.
DR SMR; Q5NTH4; -.
DR STRING; 4530.OS02T0749300-01; -.
DR PaxDb; Q5NTH4; -.
DR PRIDE; Q5NTH4; -.
DR GeneID; 4330732; -.
DR KEGG; osa:4330732; -.
DR eggNOG; ENOG502QTKR; Eukaryota.
DR InParanoid; Q5NTH4; -.
DR OrthoDB; 1565621at2759; -.
DR BRENDA; 2.7.1.71; 4460.
DR PlantReactome; R-OSA-1119430; Chorismate biosynthesis.
DR UniPathway; UPA00053; UER00088.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0009536; C:plastid; IDA:Gramene.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004765; F:shikimate kinase activity; IDA:Gramene.
DR GO; GO:0009073; P:aromatic amino acid family biosynthetic process; IDA:Gramene.
DR GO; GO:0008652; P:cellular amino acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009423; P:chorismate biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0019632; P:shikimate metabolic process; IDA:UniProtKB.
DR CDD; cd00464; SK; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00109; Shikimate_kinase; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR031322; Shikimate/glucono_kinase.
DR InterPro; IPR000623; Shikimate_kinase/TSH1.
DR InterPro; IPR023000; Shikimate_kinase_CS.
DR Pfam; PF01202; SKI; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS01128; SHIKIMATE_KINASE; 1.
PE 1: Evidence at protein level;
KW Amino-acid biosynthesis; Aromatic amino acid biosynthesis; ATP-binding;
KW Chloroplast; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Plastid;
KW Reference proteome; Transferase; Transit peptide.
FT TRANSIT 1..62
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 63..308
FT /note="Shikimate kinase 1, chloroplastic"
FT /id="PRO_0000421113"
FT BINDING 103..110
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 110
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 128
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 153
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 175
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 214
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 308 AA; 33310 MW; D8A4FDB1CE88EA3E CRC64;
MEAGVGLALQ SRAAGFGGSD RRRSALYGGE GRARIGSLRV AEPAVAKAAV WARGSKPVAP
LRAKKSSGGH ETLHNSVDEA LLLKRKSEEV LFYLNGRCIY LVGMMGSGKS TVGKIMSEVL
GYSFFDSDKL VEQAVGMPSV AQIFKVHSEA FFRDNESSVL RDLSSMKRLV VATGGGAVIR
PVNWKYMKKG LSVWLDVPLD ALARRIAKVG TASRPLLDQP SGDPYTMAFS KLSMLAEQRG
DAYANADVRV SLEEIASKQG HDDVSKLTPT DIAIESFHKI ENFVIEHTVD NPVGDSQADS
RAQRIQTL
