ID   SKA1_DANRE              Reviewed;         258 AA.
AC   Q6DHG8;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Spindle and kinetochore-associated protein 1;
GN   Name=ska1; ORFNames=zgc:92321;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the SKA1 complex, a microtubule-binding
CC       subcomplex of the outer kinetochore that is essential for proper
CC       chromosome segregation. Required for timely anaphase onset during
CC       mitosis, when chromosomes undergo bipolar attachment on spindle
CC       microtubules leading to silencing of the spindle checkpoint. The SKA1
CC       complex is a direct component of the kinetochore-microtubule interface
CC       and directly associates with microtubules as oligomeric assemblies. The
CC       complex facilitates the processive movement of microspheres along a
CC       microtubule in a depolymerization-coupled manner. Affinity for
CC       microtubules is synergistically enhanced in the presence of the ndc-80
CC       complex and may allow the ndc-80 complex to track depolymerizing
CC       microtubules. In the complex, it mediates the interaction with
CC       microtubules. {ECO:0000250|UniProtKB:Q96BD8}.
CC   -!- SUBUNIT: Component of the SKA1 complex, composed of ska1, ska2 and
CC       ska3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC       {ECO:0000250|UniProtKB:Q96BD8}. Chromosome, centromere, kinetochore
CC       {ECO:0000250|UniProtKB:Q96BD8}. Note=Localizes to the outer kinetochore
CC       and spindle microtubules during mitosis in a NDC80 complex-dependent
CC       manner. Localizes to both the mitotic spindle and kinetochore-
CC       associated proteins. Associates with kinetochores following microtubule
CC       attachment from prometaphase, through mid-anaphase and then vanishes in
CC       telophase. {ECO:0000250|UniProtKB:Q96BD8}.
CC   -!- SIMILARITY: Belongs to the SKA1 family. {ECO:0000305}.
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DR   EMBL; BC076006; AAH76006.1; -; mRNA.
DR   RefSeq; NP_001002592.1; NM_001002592.1.
DR   RefSeq; XP_005155684.1; XM_005155627.3.
DR   AlphaFoldDB; Q6DHG8; -.
DR   SMR; Q6DHG8; -.
DR   STRING; 7955.ENSDARP00000057512; -.
DR   PaxDb; Q6DHG8; -.
DR   Ensembl; ENSDART00000057513; ENSDARP00000057512; ENSDARG00000039354.
DR   Ensembl; ENSDART00000145077; ENSDARP00000117308; ENSDARG00000039354.
DR   GeneID; 436865; -.
DR   KEGG; dre:436865; -.
DR   CTD; 220134; -.
DR   ZFIN; ZDB-GENE-040718-334; ska1.
DR   eggNOG; KOG4832; Eukaryota.
DR   GeneTree; ENSGT00940000166216; -.
DR   HOGENOM; CLU_096842_0_0_1; -.
DR   InParanoid; Q6DHG8; -.
DR   OMA; YQEQTNH; -.
DR   OrthoDB; 1354933at2759; -.
DR   PhylomeDB; Q6DHG8; -.
DR   TreeFam; TF324442; -.
DR   Reactome; R-DRE-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR   Reactome; R-DRE-2467813; Separation of Sister Chromatids.
DR   Reactome; R-DRE-2500257; Resolution of Sister Chromatid Cohesion.
DR   Reactome; R-DRE-5663220; RHO GTPases Activate Formins.
DR   Reactome; R-DRE-9648025; EML4 and NUDC in mitotic spindle formation.
DR   PRO; PR:Q6DHG8; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 10.
DR   Bgee; ENSDARG00000039354; Expressed in testis and 26 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0072686; C:mitotic spindle; IBA:GO_Central.
DR   GO; GO:0000940; C:outer kinetochore; ISS:UniProtKB.
DR   GO; GO:0005876; C:spindle microtubule; ISS:UniProtKB.
DR   GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR   GO; GO:0051301; P:cell division; ISS:UniProtKB.
DR   GO; GO:0007059; P:chromosome segregation; ISS:UniProtKB.
DR   GO; GO:0000278; P:mitotic cell cycle; ISS:UniProtKB.
DR   GO; GO:0031110; P:regulation of microtubule polymerization or depolymerization; ISS:UniProtKB.
DR   Gene3D; 1.10.10.1890; -; 1.
DR   InterPro; IPR009829; SKA1.
DR   InterPro; IPR042031; SKA1-MBD.
DR   PANTHER; PTHR28573; PTHR28573; 1.
DR   Pfam; PF07160; SKA1; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Cytoplasm;
KW   Cytoskeleton; Kinetochore; Microtubule; Mitosis; Reference proteome.
FT   CHAIN           1..258
FT                   /note="Spindle and kinetochore-associated protein 1"
FT                   /id="PRO_0000273157"
FT   REGION          86..136
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          137..258
FT                   /note="Microtubule binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q96BD8"
FT   COILED          5..25
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        106..121
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   258 AA;  29669 MW;  76C65CE223DD0BE1 CRC64;
     MNHCELEEVT QHINNKISMI KRLLELRAVA KDPDKRGTLL KIGQEVSAIN ELLDRFEKYV
     GKQRDLLKHL KDLEGFFQED EQDAYHLKNN TPPHMPKRGQ QAAPQGGGQV AVQSRQTDAP
     AAPQEQGPPR KTQRNQIKEM EFITVPEFDS IPPYMKGRVT YDQLNAAVQS INTAVTSKYK
     ILHQPVKTLN NVSRSLQQRF KDQETKDTKG HFFVVEQDIK EFAQLKVDKR FVGMLNMLRH
     CQRLKEVRGG GLTRFILL