ID   SKA2_MACFA              Reviewed;         121 AA.
AC   Q4R8E8;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Spindle and kinetochore-associated protein 2;
DE   AltName: Full=Protein FAM33A;
GN   Name=SKA2; Synonyms=FAM33A; ORFNames=QtsA-12610;
OS   Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   International consortium for macaque cDNA sequencing and analysis;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the SKA1 complex, a microtubule-binding
CC       subcomplex of the outer kinetochore that is essential for proper
CC       chromosome segregation. Required for timely anaphase onset during
CC       mitosis, when chromosomes undergo bipolar attachment on spindle
CC       microtubules leading to silencing of the spindle checkpoint. The SKA1
CC       complex is a direct component of the kinetochore-microtubule interface
CC       and directly associates with microtubules as oligomeric assemblies. The
CC       complex facilitates the processive movement of microspheres along a
CC       microtubule in a depolymerization-coupled manner. In the complex, it is
CC       required for SKA1 localization. Affinity for microtubules is
CC       synergistically enhanced in the presence of the ndc-80 complex and may
CC       allow the ndc-80 complex to track depolymerizing microtubules.
CC       {ECO:0000250|UniProtKB:Q8WVK7}.
CC   -!- SUBUNIT: Component of the SKA1 complex, composed of SKA1, SKA2 and
CC       SKA3. Forms a heterodimer with SKA1; the heterodimer interacting with
CC       SKA3. The core SKA1 complex is composed of 2 SKA1-SKA2 heterodimers,
CC       each heterodimer interacting with a molecule of the SKA3 homodimer. The
CC       core SKA1 complex associates with microtubules and forms oligomeric
CC       assemblies. Interacts directly with SKA1. Binds directly to
CC       microtubules; but with a much lower affinity than SKA1 in vivo (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC       {ECO:0000250|UniProtKB:Q8WVK7}. Chromosome, centromere, kinetochore
CC       {ECO:0000250|UniProtKB:Q8WVK7}. Note=Localizes to the outer kinetochore
CC       and spindle microtubules during mitosis in a NDC80 complex-dependent
CC       manner. Localizes to both the mitotic spindle and kinetochore-
CC       associated proteins. {ECO:0000250|UniProtKB:Q8WVK7}.
CC   -!- SIMILARITY: Belongs to the SKA2 family. {ECO:0000305}.
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DR   EMBL; AB168504; BAE00624.1; -; mRNA.
DR   RefSeq; NP_001272119.1; NM_001285190.1.
DR   AlphaFoldDB; Q4R8E8; -.
DR   SMR; Q4R8E8; -.
DR   STRING; 9541.XP_005583919.1; -.
DR   GeneID; 101865188; -.
DR   CTD; 348235; -.
DR   eggNOG; ENOG502S6SM; Eukaryota.
DR   OrthoDB; 1529470at2759; -.
DR   Proteomes; UP000233100; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0000940; C:outer kinetochore; ISS:UniProtKB.
DR   GO; GO:0005876; C:spindle microtubule; ISS:UniProtKB.
DR   GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR   GO; GO:0051301; P:cell division; ISS:UniProtKB.
DR   GO; GO:0007059; P:chromosome segregation; ISS:UniProtKB.
DR   GO; GO:0000278; P:mitotic cell cycle; ISS:UniProtKB.
DR   GO; GO:0031110; P:regulation of microtubule polymerization or depolymerization; ISS:UniProtKB.
DR   InterPro; IPR026762; Ska2.
DR   InterPro; IPR042091; Ska2_N.
DR   PANTHER; PTHR32017; PTHR32017; 1.
DR   Pfam; PF16740; SKA2; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Cytoplasm; Cytoskeleton;
KW   Kinetochore; Microtubule; Mitosis; Phosphoprotein; Reference proteome.
FT   CHAIN           1..121
FT                   /note="Spindle and kinetochore-associated protein 2"
FT                   /id="PRO_0000266036"
FT   MOD_RES         101
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8WVK7"
SQ   SEQUENCE   121 AA;  14068 MW;  36E56D7F0CA0DFA6 CRC64;
     MEAEVDKLEL MFQKAESDLD YIQYRLEYEI KTNHPDSASE KNPVTLLKEL SALKSRYQTL
     YARFKPVAVE QKETKSRICA TVNKTMNVIQ KLQKQTDLEL SPLTKEEKTA AEQFKSHMPD
     L