ID   SKA2_RAT                Reviewed;         144 AA.
AC   Q5I0J4;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Spindle and kinetochore-associated protein 2;
DE   AltName: Full=Protein FAM33A;
GN   Name=Ska2; Synonyms=Fam33a;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Spleen;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Component of the SKA1 complex, a microtubule-binding
CC       subcomplex of the outer kinetochore that is essential for proper
CC       chromosome segregation. Required for timely anaphase onset during
CC       mitosis, when chromosomes undergo bipolar attachment on spindle
CC       microtubules leading to silencing of the spindle checkpoint. The SKA1
CC       complex is a direct component of the kinetochore-microtubule interface
CC       and directly associates with microtubules as oligomeric assemblies. The
CC       complex facilitates the processive movement of microspheres along a
CC       microtubule in a depolymerization-coupled manner. In the complex, it is
CC       required for SKA1 localization. Affinity for microtubules is
CC       synergistically enhanced in the presence of the ndc-80 complex and may
CC       allow the ndc-80 complex to track depolymerizing microtubules.
CC       {ECO:0000250|UniProtKB:Q8WVK7}.
CC   -!- SUBUNIT: Component of the SKA1 complex, composed of SKA1, SKA2 and
CC       SKA3. Forms a heterodimer with SKA1; the heterodimer interacting with
CC       SKA3. The core SKA1 complex is composed of 2 SKA1-SKA2 heterodimers,
CC       each heterodimer interacting with a molecule of the SKA3 homodimer. The
CC       core SKA1 complex associates with microtubules and forms oligomeric
CC       assemblies. Interacts directly with SKA1. Binds directly to
CC       microtubules; but with a much lower affinity than SKA1 in vivo (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC       {ECO:0000250|UniProtKB:Q8WVK7}. Chromosome, centromere, kinetochore
CC       {ECO:0000250|UniProtKB:Q8WVK7}. Note=Localizes to the outer kinetochore
CC       and spindle microtubules during mitosis in a NDC80 complex-dependent
CC       manner. Localizes to both the mitotic spindle and kinetochore-
CC       associated proteins. {ECO:0000250|UniProtKB:Q8WVK7}.
CC   -!- SIMILARITY: Belongs to the SKA2 family. {ECO:0000305}.
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DR   EMBL; BC088264; AAH88264.1; -; mRNA.
DR   RefSeq; NP_001009624.1; NM_001009624.1.
DR   AlphaFoldDB; Q5I0J4; -.
DR   SMR; Q5I0J4; -.
DR   STRING; 10116.ENSRNOP00000033780; -.
DR   PaxDb; Q5I0J4; -.
DR   Ensembl; ENSRNOT00000036098; ENSRNOP00000033780; ENSRNOG00000025981.
DR   GeneID; 287598; -.
DR   KEGG; rno:287598; -.
DR   CTD; 348235; -.
DR   RGD; 1307084; Ska2.
DR   eggNOG; ENOG502S6SM; Eukaryota.
DR   GeneTree; ENSGT00390000009588; -.
DR   HOGENOM; CLU_143881_0_0_1; -.
DR   InParanoid; Q5I0J4; -.
DR   OMA; MSMEPSA; -.
DR   OrthoDB; 1529470at2759; -.
DR   PhylomeDB; Q5I0J4; -.
DR   TreeFam; TF332958; -.
DR   Reactome; R-RNO-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR   Reactome; R-RNO-2467813; Separation of Sister Chromatids.
DR   Reactome; R-RNO-2500257; Resolution of Sister Chromatid Cohesion.
DR   Reactome; R-RNO-5663220; RHO GTPases Activate Formins.
DR   Reactome; R-RNO-68877; Mitotic Prometaphase.
DR   Reactome; R-RNO-9648025; EML4 and NUDC in mitotic spindle formation.
DR   PRO; PR:Q5I0J4; -.
DR   Proteomes; UP000002494; Chromosome 10.
DR   Bgee; ENSRNOG00000025981; Expressed in thymus and 19 other tissues.
DR   Genevisible; Q5I0J4; RN.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0000776; C:kinetochore; ISO:RGD.
DR   GO; GO:0000940; C:outer kinetochore; ISS:UniProtKB.
DR   GO; GO:0005876; C:spindle microtubule; ISS:UniProtKB.
DR   GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR   GO; GO:0051301; P:cell division; ISS:UniProtKB.
DR   GO; GO:0007059; P:chromosome segregation; ISS:UniProtKB.
DR   GO; GO:0000278; P:mitotic cell cycle; ISS:UniProtKB.
DR   GO; GO:0031110; P:regulation of microtubule polymerization or depolymerization; ISS:UniProtKB.
DR   InterPro; IPR026762; Ska2.
DR   InterPro; IPR042091; Ska2_N.
DR   PANTHER; PTHR32017; PTHR32017; 1.
DR   Pfam; PF16740; SKA2; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Cytoplasm; Cytoskeleton;
KW   Kinetochore; Microtubule; Mitosis; Reference proteome.
FT   CHAIN           1..144
FT                   /note="Spindle and kinetochore-associated protein 2"
FT                   /id="PRO_0000273162"
SQ   SEQUENCE   144 AA;  16517 MW;  ED5B3E12BCE98D7C CRC64;
     MEAEVDKLEL MFQKADSDLD YLQYRLEYEV KINHPHSAGE KNAVAILKEL SAIKSRYQAL
     CARFKTVSVE QKETKSCICA ILNKTMTMLQ ELQKQTNLEL TLLTEEEKAV TERLKSHVPD
     VENGILQRNF DGEKISSKEI QRGV