ID   SKA2_SALSA              Reviewed;         228 AA.
AC   B5X5N3; B5XA91;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   03-AUG-2022, entry version 44.
DE   RecName: Full=Spindle and kinetochore-associated protein 2;
DE   AltName: Full=Protein FAM33A;
GN   Name=ska2; Synonyms=fam33a;
OS   Salmo salar (Atlantic salmon).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC   Salmonidae; Salmoninae; Salmo.
OX   NCBI_TaxID=8030;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Brain;
RX   PubMed=20433749; DOI=10.1186/1471-2164-11-279;
RA   Leong J.S., Jantzen S.G., von Schalburg K.R., Cooper G.A., Messmer A.M.,
RA   Liao N.Y., Munro S., Moore R., Holt R.A., Jones S.J., Davidson W.S.,
RA   Koop B.F.;
RT   "Salmo salar and Esox lucius full-length cDNA sequences reveal changes in
RT   evolutionary pressures on a post-tetraploidization genome.";
RL   BMC Genomics 11:279-279(2010).
CC   -!- FUNCTION: Component of the SKA1 complex, a microtubule-binding
CC       subcomplex of the outer kinetochore that is essential for proper
CC       chromosome segregation. Required for timely anaphase onset during
CC       mitosis, when chromosomes undergo bipolar attachment on spindle
CC       microtubules leading to silencing of the spindle checkpoint. The SKA1
CC       complex is a direct component of the kinetochore-microtubule interface
CC       and directly associates with microtubules as oligomeric assemblies. The
CC       complex facilitates the processive movement of microspheres along a
CC       microtubule in a depolymerization-coupled manner. In the complex, it is
CC       required for ska1 localization. Affinity for microtubules is
CC       synergistically enhanced in the presence of the ndc-80 complex and may
CC       allow the ndc-80 complex to track depolymerizing microtubules.
CC       {ECO:0000250|UniProtKB:Q8WVK7}.
CC   -!- SUBUNIT: Component of the SKA1 complex, composed of ska1, ska2 and
CC       ska3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC       {ECO:0000250|UniProtKB:Q8WVK7}. Chromosome, centromere, kinetochore
CC       {ECO:0000250|UniProtKB:Q8WVK7}. Note=Localizes to the outer kinetochore
CC       and spindle microtubules during mitosis in a NDC80 complex-dependent
CC       manner. Localizes to both the mitotic spindle and kinetochore-
CC       associated proteins. {ECO:0000250|UniProtKB:Q8WVK7}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=B5X5N3-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=B5X5N3-2; Sequence=VSP_037278;
CC   -!- SIMILARITY: Belongs to the SKA2 family. {ECO:0000305}.
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DR   EMBL; BT046352; ACI66153.1; -; mRNA.
DR   EMBL; BT047960; ACI67761.1; -; mRNA.
DR   RefSeq; NP_001134080.1; NM_001140608.1. [B5X5N3-1]
DR   AlphaFoldDB; B5X5N3; -.
DR   SMR; B5X5N3; -.
DR   STRING; 8030.ENSSSAP00000017182; -.
DR   GeneID; 100195579; -.
DR   KEGG; sasa:100195579; -.
DR   CTD; 348235; -.
DR   OMA; KSLCAQM; -.
DR   Proteomes; UP000087266; Chromosome ssa13.
DR   Bgee; ENSSSAG00000008209; Expressed in ovary and 15 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0000940; C:outer kinetochore; ISS:UniProtKB.
DR   GO; GO:0005876; C:spindle microtubule; ISS:UniProtKB.
DR   GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR   GO; GO:0051301; P:cell division; ISS:UniProtKB.
DR   GO; GO:0007059; P:chromosome segregation; ISS:UniProtKB.
DR   GO; GO:0000278; P:mitotic cell cycle; ISS:UniProtKB.
DR   GO; GO:0031110; P:regulation of microtubule polymerization or depolymerization; ISS:UniProtKB.
DR   InterPro; IPR026762; Ska2.
DR   InterPro; IPR042091; Ska2_N.
DR   PANTHER; PTHR32017; PTHR32017; 1.
DR   Pfam; PF16740; SKA2; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome;
KW   Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis;
KW   Reference proteome.
FT   CHAIN           1..228
FT                   /note="Spindle and kinetochore-associated protein 2"
FT                   /id="PRO_0000373896"
FT   VAR_SEQ         140..228
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:20433749"
FT                   /id="VSP_037278"
FT   CONFLICT        86
FT                   /note="T -> P (in Ref. 1; ACI67761)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   228 AA;  25785 MW;  4C0A216DE4C0C022 CRC64;
     METAVDKLEA MFQKAEADME YMEKRLRLDF LTNVPENSAA EENPMKLLEN LSAIKTRHAA
     LCTQVQEIAA EQKQSMDAIR VHLDTTVQLV QQLQQTADVE LPTVTETEQE SAEFLGLSVN
     QNTIEVPMSM ELPAQELPQS SREGEFEEVS EATLEAVPCS MRANVKLANL NAFYKQLHEY
     FSFRKNSGAL SLPKMKQMNM KVSDAKLKTL QHLSLIELDM KGHVRLLL