ID   SKA3_XENLA              Reviewed;         556 AA.
AC   B1H1S4;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 42.
DE   RecName: Full=Spindle and kinetochore-associated protein 3;
GN   Name=ska3; Synonyms=rama1;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Ovary;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the SKA1 complex, a microtubule-binding
CC       subcomplex of the outer kinetochore that is essential for proper
CC       chromosome segregation. The SKA1 complex is a direct component of the
CC       kinetochore-microtubule interface and directly associates with
CC       microtubules as oligomeric assemblies. The complex facilitates the
CC       processive movement of microspheres along a microtubule in a
CC       depolymerization-coupled manner. In the complex, it mediates the
CC       microtubule-stimulated oligomerization. Affinity for microtubules is
CC       synergistically enhanced in the presence of the ndc-80 complex and may
CC       allow the ndc-80 complex to track depolymerizing microtubules.
CC       {ECO:0000250|UniProtKB:Q8IX90}.
CC   -!- SUBUNIT: Component of the SKA1 complex, composed of ska1, ska2 and
CC       ska3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC       {ECO:0000250|UniProtKB:Q8IX90}. Chromosome, centromere, kinetochore
CC       {ECO:0000250|UniProtKB:Q8IX90}. Note=Localizes to the outer kinetochore
CC       and spindle microtubules during mitosis in a NDC80 complex-dependent
CC       manner. {ECO:0000250|UniProtKB:Q8IX90}.
CC   -!- SIMILARITY: Belongs to the SKA3 family. {ECO:0000305}.
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DR   EMBL; BC160719; AAI60719.1; -; mRNA.
DR   RefSeq; NP_001121221.1; NM_001127749.1.
DR   AlphaFoldDB; B1H1S4; -.
DR   SMR; B1H1S4; -.
DR   GeneID; 100158292; -.
DR   KEGG; xla:100158292; -.
DR   CTD; 100158292; -.
DR   Xenbase; XB-GENE-994921; ska3.L.
DR   OrthoDB; 1070468at2759; -.
DR   Proteomes; UP000186698; Chromosome 2L.
DR   Bgee; 100158292; Expressed in testis and 15 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR   GO; GO:0000940; C:outer kinetochore; ISS:UniProtKB.
DR   GO; GO:0005876; C:spindle microtubule; ISS:UniProtKB.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; ISS:UniProtKB.
DR   GO; GO:0000278; P:mitotic cell cycle; ISS:UniProtKB.
DR   GO; GO:0031110; P:regulation of microtubule polymerization or depolymerization; ISS:UniProtKB.
DR   InterPro; IPR033341; SKA3.
DR   PANTHER; PTHR48118; PTHR48118; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Cytoplasm; Cytoskeleton;
KW   Kinetochore; Microtubule; Mitosis; Reference proteome.
FT   CHAIN           1..556
FT                   /note="Spindle and kinetochore-associated protein 3"
FT                   /id="PRO_0000373899"
FT   REGION          105..135
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        114..135
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   556 AA;  62718 MW;  F3C33D2E396960E7 CRC64;
     MSVTGNFFSK LRSLALTLEK ETAQLEQVFG NEDNEYEEES PMRVLHDLRS DIMVLKGDFQ
     STLDKKLTRG QELSAFIKAC RVLHERSAAD IEQIKETFQT YGYKPLCNDN SENEQTQESE
     KPKDNDVSPR KADAEDLPIL EKAASTSWDL LRAPQLSDFG LSHYQLPTAW EPPKVKPCPK
     NPEEEKPRIM YKDPEPINVA KTPKCALRLE EDFSQIQHFG ISDCSTNLND DYTIALINKN
     FQKKQNDTEN ETCQAKNLKS LLATPSHLSH RMDCVDSPLP PVFFTPGLKV HKKETISLPG
     KTGERQGLNA ADTDSTLLSG TCDRKDSDNA HLEDVHECTA MKPAMDLKSI SAAPPYLSLG
     SNFDSVYSPR PPVFCTPGLK THKKETSSVL VEPKEVTGCS DTPPLPSFQT NWLKNDTTEK
     ALDITEPVPR PELSYRQYLE EPPSLVLNSN KYEAPAMTEY DIGTPTRPEM TVSLTEDLFK
     YNVKPSSPPK MSEYEKMLWT PIRPEMTSCI TEDISQILSK YCDNNTNVAD KEMWNKAGAS
     FGKISSEYED KENRQY