ID   SKA3_XENTR              Reviewed;         561 AA.
AC   B0BM16;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 2.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Spindle and kinetochore-associated protein 3;
GN   Name=ska3; Synonyms=rama1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the SKA1 complex, a microtubule-binding
CC       subcomplex of the outer kinetochore that is essential for proper
CC       chromosome segregation. The SKA1 complex is a direct component of the
CC       kinetochore-microtubule interface and directly associates with
CC       microtubules as oligomeric assemblies. The complex facilitates the
CC       processive movement of microspheres along a microtubule in a
CC       depolymerization-coupled manner. In the complex, it mediates the
CC       microtubule-stimulated oligomerization. Affinity for microtubules is
CC       synergistically enhanced in the presence of the ndc-80 complex and may
CC       allow the ndc-80 complex to track depolymerizing microtubules.
CC       {ECO:0000250|UniProtKB:Q8IX90}.
CC   -!- SUBUNIT: Component of the SKA1 complex, composed of ska1, ska2 and
CC       ska3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle
CC       {ECO:0000250|UniProtKB:Q8IX90}. Chromosome, centromere, kinetochore
CC       {ECO:0000250|UniProtKB:Q8IX90}. Note=Localizes to the outer kinetochore
CC       and spindle microtubules during mitosis in a NDC80 complex-dependent
CC       manner. {ECO:0000250|UniProtKB:Q8IX90}.
CC   -!- SIMILARITY: Belongs to the SKA3 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAI58249.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; BC158248; AAI58249.1; ALT_INIT; mRNA.
DR   RefSeq; NP_001119989.1; NM_001126517.1.
DR   RefSeq; XP_012811876.1; XM_012956422.2.
DR   RefSeq; XP_012811877.1; XM_012956423.2.
DR   RefSeq; XP_012811878.1; XM_012956424.2.
DR   RefSeq; XP_012811879.1; XM_012956425.2.
DR   RefSeq; XP_012811880.1; XM_012956426.1.
DR   RefSeq; XP_017946565.1; XM_018091076.1.
DR   RefSeq; XP_017946566.1; XM_018091077.1.
DR   RefSeq; XP_017946567.1; XM_018091078.1.
DR   AlphaFoldDB; B0BM16; -.
DR   SMR; B0BM16; -.
DR   STRING; 8364.ENSXETP00000021155; -.
DR   PaxDb; B0BM16; -.
DR   GeneID; 100144945; -.
DR   KEGG; xtr:100144945; -.
DR   CTD; 221150; -.
DR   Xenbase; XB-GENE-994918; ska3.
DR   eggNOG; ENOG502QSTX; Eukaryota.
DR   HOGENOM; CLU_033334_1_0_1; -.
DR   InParanoid; B0BM16; -.
DR   OrthoDB; 1070468at2759; -.
DR   TreeFam; TF332721; -.
DR   Proteomes; UP000008143; Chromosome 2.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR   GO; GO:0000940; C:outer kinetochore; ISS:UniProtKB.
DR   GO; GO:0005876; C:spindle microtubule; ISS:UniProtKB.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; ISS:UniProtKB.
DR   GO; GO:0000278; P:mitotic cell cycle; ISS:UniProtKB.
DR   GO; GO:0031110; P:regulation of microtubule polymerization or depolymerization; ISS:UniProtKB.
DR   InterPro; IPR033341; SKA3.
DR   PANTHER; PTHR48118; PTHR48118; 2.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Cytoplasm; Cytoskeleton;
KW   Kinetochore; Microtubule; Mitosis; Reference proteome.
FT   CHAIN           1..561
FT                   /note="Spindle and kinetochore-associated protein 3"
FT                   /id="PRO_0000373900"
FT   REGION          105..131
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          304..327
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          391..413
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        313..327
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   561 AA;  62908 MW;  95E88A871943C79B CRC64;
     MSVTGNFFSK LRSLAVTLEK ETGQLEQLFS SEDNEYEEES PMRVLHDLRS DIMVLKGDFQ
     STLNKRLARG QELSAFIKAC RVLHERTAAD IEQIKETFQT YGYKPQCNAN SEREQTQESG
     KPKENGVSPN KADVEDLQAL PTLEKTASTS WDILRAPQLS DFGLAHYQLP TAWEPPKVKP
     CKKDPEEEKP RIMLKDPEPM NVAKTPKCAL RLEEDFSQIQ HFGISDCSTN LNDDYTMALI
     NKKFQKNQNV TENAAKETCQ PKNLKSLLAT PSHLSHRMDW VDSPLPPVFC TPGLKVHKKE
     KMSLPGKPAE KKGLNANNTT LPSDTCDRND PDSAHLEDDY ECTAVKPAID FKSTSAAPAY
     LSLGSNINSV YSPRPPVFCT PGLKVHKKET SSVLADPTEP KGTGCTDTPP LPSFQTNWLK
     SDAKEKGLDI TEPVPRPDLS YRQCLEEPPT LVLNSNKYEA PAMRDYCMGT PPRPEMTVSL
     TEDLFKYNVK PSSPPKVSEY ENMLWTPVRP EMTSCITEDI SQILSKYCDN NMNPADGIWD
     KAGAPFGKIS TEYENKENRL Y