ID   BHE22_MESAU             Reviewed;         365 AA.
AC   O09029;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 2.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=Class E basic helix-loop-helix protein 22;
DE            Short=bHLHe22;
DE   AltName: Full=Beta-cell E-box transcriptional activator 3;
DE            Short=BETA3;
DE   AltName: Full=Class B basic helix-loop-helix protein 5;
DE            Short=bHLHb5;
GN   Name=BHLHE22; Synonyms=BHLHB5;
OS   Mesocricetus auratus (Golden hamster).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC   Cricetidae; Cricetinae; Mesocricetus.
OX   NCBI_TaxID=10036;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8552091; DOI=10.1128/mcb.16.2.626;
RA   Peyton M., Stellrecht C.M.M., Naya F.J., Huang H.-P., Samora P.J.,
RA   Tsai M.-J.;
RT   "BETA3, a novel helix-loop-helix protein, can act as a negative regulator
RT   of BETA2 and MyoD-responsive genes.";
RL   Mol. Cell. Biol. 16:626-633(1996).
CC   -!- FUNCTION: Inhibits DNA binding of TCF3/E47 homodimers and TCF3
CC       (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1
CC       and Myod-responsive genes, probably by heterodimerization with class a
CC       basic helix-loop-helix factors. Despite the presence of an intact basic
CC       domain, does not bind to DNA.
CC   -!- SUBUNIT: Heterodimer with other bHLH proteins, like TCF3/E47.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC   -!- TISSUE SPECIFICITY: Kidney, lung, brain and pancreas (insulinoma).
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB50691.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; S80870; AAB50691.1; ALT_INIT; mRNA.
DR   AlphaFoldDB; O09029; -.
DR   SMR; O09029; -.
DR   STRING; 10036.XP_005066276.1; -.
DR   eggNOG; KOG3898; Eukaryota.
DR   Proteomes; UP000189706; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0022008; P:neurogenesis; IEA:InterPro.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR032654; Bhlhe22.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   PANTHER; PTHR19290:SF52; PTHR19290:SF52; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   2: Evidence at transcript level;
KW   Nucleus; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..365
FT                   /note="Class E basic helix-loop-helix protein 22"
FT                   /id="PRO_0000127143"
FT   DOMAIN          226..280
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          34..93
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          134..156
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          188..225
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   365 AA;  35703 MW;  EADB83DCF2EE4DB9 CRC64;
     MERGLHLGAA AASEDDLFLH KSLGASTAKR LEAAFRSTPP GMDLSLAPPP RERPASSSSS
     PLGCFEPADP EGAGLLLPPP GGGGGAGGGG GGGGGGGVSV PGLLVGSAGV GGDPNLSSLP
     AGAALCLKYG ESAGRGSVAE SSGGEQSPDD DSDGRCELVL RAGGADPRAS PGAGGGGTKV
     VEGCSNAHLH GGAGLPPGGS TGSGGGGSGG GGGGGSSSKK SKEQKALRLN INARERRRMH
     DLNDALDELR AVIPYAHSPS VRKLSKIATL LLAKNYILMQ AQALEEMRRL VAYLNQGQAI
     SAASLPSSAA AAAAAAALHP ALGAYEQAAG YPFSAGLPPA ASCPEKCALF NSVSSSLCKQ
     CTEKP