SKI15_ARATH
ID SKI15_ARATH Reviewed; 374 AA.
AC O49279;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=SKP1-interacting partner 15;
DE AltName: Full=F-box only protein 3;
GN Name=SKIP15; Synonyms=FBX3; OrderedLocusNames=At1g76920; ORFNames=F22K20.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11077244; DOI=10.1016/s1360-1385(00)01769-6;
RA Xiao W., Jang J.-C.;
RT "F-box proteins in Arabidopsis.";
RL Trends Plant Sci. 5:454-457(2000).
RN [6]
RP INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK11 AND ASK13.
RX PubMed=12169662; DOI=10.1073/pnas.162339999;
RA Gagne J.M., Downes B.P., Shiu S.-H., Durski A.M., Vierstra R.D.;
RT "The F-box subunit of the SCF E3 complex is encoded by a diverse
RT superfamily of genes in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:11519-11524(2002).
RN [7]
RP INTERACTION WITH SKPIB/ASK2.
RX PubMed=12795696; DOI=10.1046/j.1365-313x.2003.01768.x;
RA Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J.,
RA Hellmann H., Estelle M., Somers D.E., Crosby W.L.;
RT "Protein interaction analysis of SCF ubiquitin E3 ligase subunits from
RT Arabidopsis.";
RL Plant J. 34:753-767(2003).
CC -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC complexes, which may mediate the ubiquitination and subsequent
CC proteasomal degradation of target proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (ASK-cullin-F-box) protein ligase complex (By
CC similarity). Interacts with SKP1A/ASK1, SKP1B/ASK2, ASK11 and ASK13.
CC {ECO:0000250, ECO:0000269|PubMed:12169662,
CC ECO:0000269|PubMed:12795696}.
CC -!- INTERACTION:
CC O49279; O49484: ASK11; NbExp=3; IntAct=EBI-591174, EBI-401185;
CC O49279; Q39255: SKP1A; NbExp=4; IntAct=EBI-591174, EBI-532357;
CC O49279; Q9FHW7: SKP1B; NbExp=6; IntAct=EBI-591174, EBI-604076;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The F-box is necessary for the interaction with ASK proteins.
CC {ECO:0000250}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC002291; AAC00632.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35903.1; -; Genomic_DNA.
DR EMBL; AY065102; AAL38278.1; -; mRNA.
DR EMBL; BT008455; AAP37814.1; -; mRNA.
DR EMBL; AY085473; AAM62699.1; -; mRNA.
DR PIR; A96798; A96798.
DR RefSeq; NP_565142.1; NM_106343.4.
DR AlphaFoldDB; O49279; -.
DR BioGRID; 29246; 12.
DR IntAct; O49279; 12.
DR STRING; 3702.AT1G76920.1; -.
DR PaxDb; O49279; -.
DR PRIDE; O49279; -.
DR ProteomicsDB; 234466; -.
DR EnsemblPlants; AT1G76920.1; AT1G76920.1; AT1G76920.
DR GeneID; 844027; -.
DR Gramene; AT1G76920.1; AT1G76920.1; AT1G76920.
DR KEGG; ath:AT1G76920; -.
DR Araport; AT1G76920; -.
DR TAIR; locus:2025247; AT1G76920.
DR eggNOG; ENOG502QT3E; Eukaryota.
DR HOGENOM; CLU_055529_0_0_1; -.
DR InParanoid; O49279; -.
DR OMA; GNAMPTA; -.
DR OrthoDB; 1132032at2759; -.
DR PhylomeDB; O49279; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:O49279; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O49279; baseline and differential.
DR Genevisible; O49279; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IDA:TAIR.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IDA:TAIR.
DR Gene3D; 2.120.10.80; -; 1.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR011498; Kelch_2.
DR Pfam; PF00646; F-box; 1.
DR Pfam; PF07646; Kelch_2; 1.
DR SMART; SM00256; FBOX; 1.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..374
FT /note="SKP1-interacting partner 15"
FT /id="PRO_0000273536"
FT DOMAIN 3..48
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
SQ SEQUENCE 374 AA; 42494 MW; 1D8584587C616173 CRC64;
MESSPVNCLP PDSLHQIFSS LPIRDIMICR SVCKFFNQLL TSQCFIEIIS TRPPLNLLAL
RPPHHHHSHR HSGNGHATNI RPYIHVYDPE QNQWFRFNLD FLPFRSPQPV ASSSGLIYLW
GDSIDLAESS KSLVACNPLT RQFKVLPQLG SAWSRHGTVL VDSVNRVMVL TELAALYYSG
TVVANQWLKF SSNLPSKPRS PVLMSSSVFA LCDVGSPWRS QWKLFSCKLT NLTITHTNWV
CLEKHEWGDI FDIIKRPRLL RGNGDSKLLM IGGLKSTFSL NPACSTILIL RLDLESLEWE
EAGRMPLEMY RGFQESSKFK VFGGGDRVYF SAKRMGKLAM WDCWQGWRWI EGVPGYADGL
CRGFVFDAKL TLMP
