SKI20_ARATH
ID SKI20_ARATH Reviewed; 418 AA.
AC Q9M1Y1; Q8L991;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=F-box/kelch-repeat protein SKIP20;
DE AltName: Full=SKP1-interacting partner 20;
GN Name=SKIP20; OrderedLocusNames=At3g59940; ORFNames=F24G16.210;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH SKP1A/ASK1 AND SPK1B/ASK2.
RX PubMed=12795696; DOI=10.1046/j.1365-313x.2003.01768.x;
RA Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J.,
RA Hellmann H., Estelle M., Somers D.E., Crosby W.L.;
RT "Protein interaction analysis of SCF ubiquitin E3 ligase subunits from
RT Arabidopsis.";
RL Plant J. 34:753-767(2003).
CC -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC complexes, which may mediate the ubiquitination and subsequent
CC proteasomal degradation of target proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (ASK-cullin-F-box) protein ligase complex (By
CC similarity). Interacts with SKP1A/ASK1 and SPK1B/ASK2. {ECO:0000250,
CC ECO:0000269|PubMed:12795696}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The F-box is necessary for the interaction with ASK proteins.
CC {ECO:0000250}.
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DR EMBL; AL138647; CAB75813.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79987.1; -; Genomic_DNA.
DR EMBL; AY074382; AAL67078.1; -; mRNA.
DR EMBL; AY081325; AAL91214.1; -; mRNA.
DR EMBL; AY091240; AAM14179.1; -; mRNA.
DR EMBL; AY088575; AAM66106.1; -; mRNA.
DR PIR; T47818; T47818.
DR RefSeq; NP_191553.1; NM_115857.2.
DR AlphaFoldDB; Q9M1Y1; -.
DR SMR; Q9M1Y1; -.
DR BioGRID; 10478; 7.
DR IntAct; Q9M1Y1; 4.
DR STRING; 3702.AT3G59940.1; -.
DR PaxDb; Q9M1Y1; -.
DR PRIDE; Q9M1Y1; -.
DR EnsemblPlants; AT3G59940.1; AT3G59940.1; AT3G59940.
DR GeneID; 825164; -.
DR Gramene; AT3G59940.1; AT3G59940.1; AT3G59940.
DR KEGG; ath:AT3G59940; -.
DR Araport; AT3G59940; -.
DR TAIR; locus:2080432; AT3G59940.
DR eggNOG; KOG1072; Eukaryota.
DR HOGENOM; CLU_028510_1_0_1; -.
DR InParanoid; Q9M1Y1; -.
DR OMA; FCECVAI; -.
DR OrthoDB; 1033096at2759; -.
DR PhylomeDB; Q9M1Y1; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9M1Y1; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M1Y1; baseline and differential.
DR Genevisible; Q9M1Y1; AT.
DR GO; GO:0005829; C:cytosol; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0080037; P:negative regulation of cytokinin-activated signaling pathway; IGI:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:2000762; P:regulation of phenylpropanoid metabolic process; IGI:TAIR.
DR Gene3D; 2.120.10.80; -; 1.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR Pfam; PF01344; Kelch_1; 1.
DR SMART; SM00256; FBOX; 1.
DR SMART; SM00612; Kelch; 2.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
PE 1: Evidence at protein level;
KW Kelch repeat; Nucleus; Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..418
FT /note="F-box/kelch-repeat protein SKIP20"
FT /id="PRO_0000283236"
FT DOMAIN 14..61
FT /note="F-box"
FT REPEAT 104..150
FT /note="Kelch 1"
FT REPEAT 153..206
FT /note="Kelch 2"
FT REPEAT 208..255
FT /note="Kelch 3"
FT REPEAT 258..314
FT /note="Kelch 4"
FT REGION 79..104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 24
FT /note="A -> P (in Ref. 4; AAM66106)"
FT /evidence="ECO:0000305"
FT CONFLICT 84
FT /note="M -> I (in Ref. 4; AAM66106)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 418 AA; 45970 MW; 1EA069B038A11323 CRC64;
MGVSKKKSGE IRGDLIPGLP EELAIECLVR VPFQFHSSIK SVCRSWKCVI SSRSFIKERI
GFGKAESLLC LVQPLTSPPS PAMMEGGEMS QKKKEEEEGE SQMTQQLLQP RITGTPLYGL
SVYNATLDTW HRVAIPERIP LFCECVAIQD AGKVLLIGGW DPETLQPVRD VFVLDFFAGE
GSGRRFRRGR PMSAARSFFA CASVGSTKVY VAGGHDDQKN ALRSAEVYDV EKDEWSMLPP
MTEGRDECHG FSMATDPGFC VLSGYGTETQ GQFRSDGEIY DPITNSWSTI ENVWPFPDLS
PRGRTAAAAA EFPGDFRGCR LWCFIDSERQ SQPHWEVEDD SMKWKVIMDT IRLPVTTMTS
VFAGSLSGQA VAMIGGGGEE SGTMMVKTTA EKNGGKWSHV NTPSGFSSLP FSCSSIYV
