SKI22_ARATH
ID SKI22_ARATH Reviewed; 475 AA.
AC Q9ZUB8; B9DHN7;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=F-box protein SKIP22;
DE AltName: Full=SKP1-interacting partner 22;
GN Name=SKIP22; OrderedLocusNames=At1g23780; ORFNames=F5O8.33;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 193-475.
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP INTERACTION WITH SKP1A/ASK1 AND SPK1B/ASK2.
RX PubMed=12795696; DOI=10.1046/j.1365-313x.2003.01768.x;
RA Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J.,
RA Hellmann H., Estelle M., Somers D.E., Crosby W.L.;
RT "Protein interaction analysis of SCF ubiquitin E3 ligase subunits from
RT Arabidopsis.";
RL Plant J. 34:753-767(2003).
CC -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC complexes, which may mediate the ubiquitination and subsequent
CC proteasomal degradation of target proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (ASK-cullin-F-box) protein ligase complex (By
CC similarity). Interacts with SKP1A/ASK1 and SPK1B/ASK2. {ECO:0000250,
CC ECO:0000269|PubMed:12795696}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The F-box is necessary for the interaction with ASK proteins.
CC {ECO:0000250}.
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DR EMBL; AC005990; AAC98033.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30431.1; -; Genomic_DNA.
DR EMBL; AY049291; AAK83633.1; -; mRNA.
DR EMBL; AY149929; AAN31083.1; -; mRNA.
DR EMBL; AK317590; BAH20254.1; -; mRNA.
DR PIR; A86372; A86372.
DR RefSeq; NP_564203.1; NM_102226.3.
DR AlphaFoldDB; Q9ZUB8; -.
DR SMR; Q9ZUB8; -.
DR BioGRID; 24227; 8.
DR IntAct; Q9ZUB8; 3.
DR STRING; 3702.AT1G23780.1; -.
DR PaxDb; Q9ZUB8; -.
DR PRIDE; Q9ZUB8; -.
DR ProteomicsDB; 232635; -.
DR EnsemblPlants; AT1G23780.1; AT1G23780.1; AT1G23780.
DR GeneID; 838989; -.
DR Gramene; AT1G23780.1; AT1G23780.1; AT1G23780.
DR KEGG; ath:AT1G23780; -.
DR Araport; AT1G23780; -.
DR TAIR; locus:2034845; AT1G23780.
DR eggNOG; ENOG502QTNJ; Eukaryota.
DR HOGENOM; CLU_037173_0_0_1; -.
DR OMA; ICLVLED; -.
DR OrthoDB; 999024at2759; -.
DR PhylomeDB; Q9ZUB8; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9ZUB8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9ZUB8; baseline and differential.
DR Genevisible; Q9ZUB8; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR Pfam; PF12937; F-box-like; 1.
DR SMART; SM00256; FBOX; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..475
FT /note="F-box protein SKIP22"
FT /id="PRO_0000283296"
FT DOMAIN 319..365
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REGION 114..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 194
FT /note="V -> M (in Ref. 4; BAH20254)"
FT /evidence="ECO:0000305"
FT CONFLICT 224
FT /note="S -> Y (in Ref. 4; BAH20254)"
FT /evidence="ECO:0000305"
FT CONFLICT 463
FT /note="Q -> E (in Ref. 4; BAH20254)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 475 AA; 52947 MW; D068CCF15448EFC3 CRC64;
MKLRLRHHET RETLKLELAD ADTLHDLRRR INPTVPSSVH LSLNRKDELI TPSPEDTLRS
LGLISGDLIY FSLEAGESSN WKLRDSETVA SQSESNQTSV HDSIGFAEVD VVPDQAKSNP
NTSVEDPEGD ISGMEGPEPM DVEQLDMELA AAGSKRLSEP FFLKNILLEK SGDTSELTTL
ALSVHAVMLE SGFVLLNHGS DKFNFSKELL TVSLRYTLPE LIKSKDTNTI ESVSVKFQNL
GPVVVVYGTV GGSSGRVHMN LDKRRFVPVI DLVMDTSTSD EEGSSSIYRE VFMFWRMVKD
RLVIPLLIGI CDKAGLEPPP CLMRLPTELK LKILELLPGV SIGNMACVCT EMRYLASDND
LWKQKCLEEV NNFVVTEAGD SVNWKARFAT FWRQKQLAAA SDTFWRQNQL GRRNISTGRS
GIRFPRIIGD PPFTWFNGDR MHGSIGIHPG QSARGLGRRT WGQLFTPRCN LGGLN
