SKI24_ARATH
ID SKI24_ARATH Reviewed; 295 AA.
AC Q9CAZ0; Q8VZ41;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=F-box protein SKIP24;
DE AltName: Full=SKP1-interacting partner 24;
GN Name=SKIP24; OrderedLocusNames=At1g08710; ORFNames=F22O13.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-295 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [5]
RP INTERACTION WITH SKP1A/ASK1 AND SPK1B/ASK2.
RX PubMed=12795696; DOI=10.1046/j.1365-313x.2003.01768.x;
RA Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J.,
RA Hellmann H., Estelle M., Somers D.E., Crosby W.L.;
RT "Protein interaction analysis of SCF ubiquitin E3 ligase subunits from
RT Arabidopsis.";
RL Plant J. 34:753-767(2003).
CC -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC complexes, which may mediate the ubiquitination and subsequent
CC proteasomal degradation of target proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (ASK-cullin-F-box) protein ligase complex (By
CC similarity). Interacts with SKP1A/ASK1 and SPK1B/ASK2. {ECO:0000250,
CC ECO:0000269|PubMed:12795696}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CAZ0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CAZ0-2; Sequence=VSP_037359;
CC -!- DOMAIN: The F-box is necessary for the interaction with ASK proteins.
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF99761.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BX814640; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC003981; AAF99761.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28337.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28338.1; -; Genomic_DNA.
DR EMBL; AY065275; AAL38751.1; -; mRNA.
DR EMBL; AY133758; AAM91692.1; -; mRNA.
DR EMBL; BX814640; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; D86219; D86219.
DR PIR; T00725; T00725.
DR RefSeq; NP_001077493.1; NM_001084024.2. [Q9CAZ0-1]
DR RefSeq; NP_172347.2; NM_100744.3. [Q9CAZ0-2]
DR AlphaFoldDB; Q9CAZ0; -.
DR SMR; Q9CAZ0; -.
DR BioGRID; 22633; 2.
DR IntAct; Q9CAZ0; 2.
DR STRING; 3702.AT1G08710.2; -.
DR PaxDb; Q9CAZ0; -.
DR PRIDE; Q9CAZ0; -.
DR ProteomicsDB; 232591; -. [Q9CAZ0-1]
DR EnsemblPlants; AT1G08710.1; AT1G08710.1; AT1G08710. [Q9CAZ0-2]
DR EnsemblPlants; AT1G08710.2; AT1G08710.2; AT1G08710. [Q9CAZ0-1]
DR GeneID; 837392; -.
DR Gramene; AT1G08710.1; AT1G08710.1; AT1G08710. [Q9CAZ0-2]
DR Gramene; AT1G08710.2; AT1G08710.2; AT1G08710. [Q9CAZ0-1]
DR KEGG; ath:AT1G08710; -.
DR Araport; AT1G08710; -.
DR TAIR; locus:2025665; AT1G08710.
DR eggNOG; ENOG502QWB0; Eukaryota.
DR InParanoid; Q9CAZ0; -.
DR OMA; NVWQPEI; -.
DR OrthoDB; 1273295at2759; -.
DR PhylomeDB; Q9CAZ0; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9CAZ0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CAZ0; baseline and differential.
DR Genevisible; Q9CAZ0; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR SUPFAM; SSF81383; SSF81383; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Reference proteome;
KW Ubl conjugation pathway.
FT CHAIN 1..295
FT /note="F-box protein SKIP24"
FT /id="PRO_0000375239"
FT DOMAIN 19..66
FT /note="F-box; degenerate"
FT REGION 217..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 82..129
FT /evidence="ECO:0000255"
FT COILED 167..209
FT /evidence="ECO:0000255"
FT COMPBIAS 220..245
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 258..295
FT /note="CKFMNISHFSSCSSVTEKFYSYSPKIIHEYIPENLLVL -> FPAKPRNTEK
FT LFQSESE (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_037359"
FT CONFLICT 28..29
FT /note="DL -> EG (in Ref. 4; BX814640)"
FT /evidence="ECO:0000305"
FT CONFLICT 136
FT /note="V -> L (in Ref. 4; BX814640)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 295 AA; 34341 MW; DC3C45148D385E63 CRC64;
MSANEIPDEL WRKILEIGVK SSTFSYKDLC CISISSRRLF RLSCDDSLWD LLLVHDFPNH
IVSASSSSES PTKFIYMTRF EREKERKLAA HRRALLRKES EISEWGRRIR ELEARLSDEA
ERLQSSSLQF SDLLKVRQAS VALNVWQPEV VRGRQKQMVE QNAVPVEGRL RALEMEMKLC
KQQIMGLNRA LREVKHRYDI AIKELESMKY HPLRDYKSIR NGDQGSNGKT KKLKTSINYS
GDQVSNGKRR KLKTSIDCKF MNISHFSSCS SVTEKFYSYS PKIIHEYIPE NLLVL
