SKI25_ARATH
ID SKI25_ARATH Reviewed; 395 AA.
AC Q8GX29; Q9SHE6;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=F-box/kelch-repeat protein SKIP25;
DE AltName: Full=SKP1-interacting partner 25;
GN Name=SKIP25; OrderedLocusNames=At1g31350; ORFNames=T19E23.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP INTERACTION WITH SKP1A/ASK1.
RX PubMed=12795696; DOI=10.1046/j.1365-313x.2003.01768.x;
RA Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J.,
RA Hellmann H., Estelle M., Somers D.E., Crosby W.L.;
RT "Protein interaction analysis of SCF ubiquitin E3 ligase subunits from
RT Arabidopsis.";
RL Plant J. 34:753-767(2003).
CC -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC complexes, which may mediate the ubiquitination and subsequent
CC proteasomal degradation of target proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (ASK-cullin-F-box) protein ligase complex (By
CC similarity). Interacts with SKP1A/ASK1. {ECO:0000250,
CC ECO:0000269|PubMed:12795696}.
CC -!- INTERACTION:
CC Q8GX29; Q9FHW7: SKP1B; NbExp=3; IntAct=EBI-604376, EBI-604076;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The F-box is necessary for the interaction with ASK proteins.
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF24593.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007654; AAF24593.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31345.1; -; Genomic_DNA.
DR EMBL; AK118467; BAC43075.1; -; mRNA.
DR EMBL; BT005351; AAO63415.1; -; mRNA.
DR RefSeq; NP_174420.1; NM_102874.4.
DR AlphaFoldDB; Q8GX29; -.
DR SMR; Q8GX29; -.
DR BioGRID; 25259; 8.
DR IntAct; Q8GX29; 8.
DR STRING; 3702.AT1G31350.1; -.
DR PaxDb; Q8GX29; -.
DR PRIDE; Q8GX29; -.
DR DNASU; 840024; -.
DR EnsemblPlants; AT1G31350.1; AT1G31350.1; AT1G31350.
DR GeneID; 840024; -.
DR Gramene; AT1G31350.1; AT1G31350.1; AT1G31350.
DR KEGG; ath:AT1G31350; -.
DR Araport; AT1G31350; -.
DR TAIR; locus:2197540; AT1G31350.
DR eggNOG; KOG1072; Eukaryota.
DR HOGENOM; CLU_040372_0_0_1; -.
DR InParanoid; Q8GX29; -.
DR PhylomeDB; Q8GX29; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q8GX29; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GX29; baseline and differential.
DR Genevisible; Q8GX29; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 2.120.10.80; -; 1.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
PE 1: Evidence at protein level;
KW Kelch repeat; Nucleus; Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..395
FT /note="F-box/kelch-repeat protein SKIP25"
FT /id="PRO_0000283179"
FT DOMAIN 34..79
FT /note="F-box"
FT REPEAT 81..129
FT /note="Kelch 1"
FT REPEAT 147..194
FT /note="Kelch 2"
FT REPEAT 196..245
FT /note="Kelch 3"
FT REPEAT 246..299
FT /note="Kelch 4"
FT REPEAT 301..342
FT /note="Kelch 5"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 12..29
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 395 AA; 44149 MW; 5183FB874F538770 CRC64;
MEKKLKRRES MSTTAAESPP AKRRRTVTGN ENSALIEGLP DHISEICLSL VHRPSLLSAV
CTRWRRLLYS PEFPSFPSLY ALFVDSTSDT GRVNPSVRFM CFNPVSSKWY PLPPPPPDPP
LHRILYRHPS FISFNLPIQC VSAAGKLILI AGSNQQLSPA ISHPLIFDPI SSSWSSGPRI
GSPRRWCATG ACDGAIYIAS GISSQFSSTV AKSVEKLDLT EQNRNNHRFN WEKLRDMRDL
RFSREAIDAV GYRRKLLMVN VKGDAVKEGA IYDVVKDDWE PMPEEMLVGW RGPVAAMEEE
ILYSVDERRG TVRKYDDEKR EWREVVVVEG GEEMLKGATQ VTADSGKLCV VTGDGKIVVV
DVAAEPAKIW NVEIPDGLEP VSVHVLPRMS QPNFC
