SKI2_SCHPO
ID SKI2_SCHPO Reviewed; 1213 AA.
AC O59801;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Putative ATP-dependent RNA helicase C550.03c;
DE EC=3.6.4.-;
GN ORFNames=SPCC550.03c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: RNA helicase component of the SKI complex involved in 3'-mRNA
CC degradation pathway. {ECO:0000250}.
CC -!- SUBUNIT: Component of the SKI complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the helicase family. SKI2 subfamily.
CC {ECO:0000305}.
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DR EMBL; CU329672; CAA19107.1; -; Genomic_DNA.
DR PIR; T41378; T41378.
DR RefSeq; NP_588095.1; NM_001023086.2.
DR AlphaFoldDB; O59801; -.
DR SMR; O59801; -.
DR BioGRID; 275422; 64.
DR STRING; 4896.SPCC550.03c.1; -.
DR iPTMnet; O59801; -.
DR MaxQB; O59801; -.
DR PaxDb; O59801; -.
DR PRIDE; O59801; -.
DR EnsemblFungi; SPCC550.03c.1; SPCC550.03c.1:pep; SPCC550.03c.
DR GeneID; 2538841; -.
DR KEGG; spo:SPCC550.03c; -.
DR PomBase; SPCC550.03c; -.
DR VEuPathDB; FungiDB:SPCC550.03c; -.
DR eggNOG; KOG0947; Eukaryota.
DR HOGENOM; CLU_002902_1_4_1; -.
DR InParanoid; O59801; -.
DR OMA; DRNIWVH; -.
DR PhylomeDB; O59801; -.
DR Reactome; R-SPO-429958; mRNA decay by 3' to 5' exoribonuclease.
DR PRO; PR:O59801; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005730; C:nucleolus; ISO:PomBase.
DR GO; GO:0055087; C:Ski complex; ISO:PomBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; ISO:PomBase.
DR GO; GO:0030968; P:endoplasmic reticulum unfolded protein response; IMP:PomBase.
DR GO; GO:0070478; P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay; ISO:PomBase.
DR GO; GO:0070481; P:nuclear-transcribed mRNA catabolic process, non-stop decay; ISO:PomBase.
DR GO; GO:0006401; P:RNA catabolic process; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR025696; rRNA_proc-arch_dom.
DR InterPro; IPR016438; Ski2-like.
DR InterPro; IPR012961; Ski2_C.
DR InterPro; IPR040801; Ski2_N.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF08148; DSHCT; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF13234; rRNA_proc-arch; 1.
DR Pfam; PF17911; Ski2_N; 1.
DR PIRSF; PIRSF005198; Antiviral_helicase_SKI2; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM01142; DSHCT; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; Helicase; Hydrolase; Nucleotide-binding;
KW Reference proteome; RNA-binding.
FT CHAIN 1..1213
FT /note="Putative ATP-dependent RNA helicase C550.03c"
FT /id="PRO_0000310785"
FT DOMAIN 289..445
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 545..736
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 494..514
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 393..396
FT /note="DEAH box"
FT BINDING 302..309
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 1213 AA; 138028 MW; 2F9BE2ABF8068A37 CRC64;
MSSKLVDAIN EVAVSNKDKI ELDGIKDDSF DGHLSITEAT YDVDTFLKPS PALSKDWIRK
LQKKWDREIT YKGLYEYPET LARTQIRFQR HGLEGKIMGY KEVPELIEDL NSKNSSSFLR
KPSSKNEFVR GSTSNIPFLA DDSDVDAIAG EPSVKMALYG EDGLLQVPPG FSRGLSMTAT
STTDNLNDEF DPEKWDTKKV KSSNRNFVTI HELNEHLKNV NSKHSEIDDL LPDKRSIVSL
PPSTLNLHKQ PDYAHVVDSS APIENFQQLV PEMALDFPFE LDNFQKEAIY HLEMGDSVFV
AAHTSAGKTV VAEYAIALAQ KHMTKAIYTS PIKALSNQKF RDFKHKFEDV GILTGDVQVN
PEGSCLLMTT EILRSMLYRG ADLIRDVEFV IFDEVHYVND LERGVVWEEV IIMLPPHVTL
ILLSATVPNT KEFASWVGRT KKKNIYVIST LKRPVPLEHY LWVKQNMFKI VDQHGRFLMD
GYKSANDALK KPDKPVIAKD NKNSARGRGA ARGRGVQTNM MRGRGSAKSV ERRDANTWVH
LIGHLHKQNL LPVIVFVFSK KRCEEYVDTL TNRDLNNHQE KSEVHVVIEK AVARLKKEDR
LLPQIGRMRE MLSRGLAVHH GGLLPIIKEI VEILFQRGLV KVLFATETFA MGVNMPAKSV
VFSGTQKHDG RNFRDLLPGE YTQCSGRAGR RGLDVTGTVI ILSRSELPDT ASLRHMIMGP
SSKLISQFRL TYNMILNLLR VETLRIEDMI KRSFSENVNQ TLVPQHEEKI KSFEEKLSAL
KKEMSDVDLK EIKSCLLSSE SFKEYTKKMH FRAITTANGK RIFKDGRVIV FQQLDFTRTV
GVLLGTSIRT NASDCTLEVA YLNPQNNLKR PSDLLAFADA FNDVYDNAIF DESNQFKYGL
INLSGIERVC NTILRIDSGG IRDRRGGAFR KLSEQFASIK KFSDLLFEEV NWSKVRDFEF
CEAFEKRNFL QNKLSGNPII STPNFLTHFA LAYQEYELES NIDNLSSYIS DQNLELLPDY
EQRIKVLQEL GYIDAERTVL LKGRVACEIN STSELVLTEL ILENSLADFS CEETIALLSA
FVFDEKTEVE PTISPHLQKG KEMILSVAEK VNQIQEHYQV LYFNEGNDFE SQPRFGLMEV
CYEWARGMSF NRITDLTDVL EGSIVRTIIR LDEVLRECRG AARVVGDSSM YTKMEECQNL
IRRNIVFCPS LYM
