SKI32_ARATH
ID SKI32_ARATH Reviewed; 328 AA.
AC Q9XI00;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=F-box protein 7;
DE AltName: Full=SKP1-interacting partner 32;
GN Name=SKIP32; Synonyms=FBP7; OrderedLocusNames=At1g21760; ORFNames=F8K7.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK4; ASK11 AND ASK14.
RX PubMed=12169662; DOI=10.1073/pnas.162339999;
RA Gagne J.M., Downes B.P., Shiu S.-H., Durski A.M., Vierstra R.D.;
RT "The F-box subunit of the SCF E3 complex is encoded by a diverse
RT superfamily of genes in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:11519-11524(2002).
RN [6]
RP INTERACTION WITH SKPIA/ASK1.
RX PubMed=12795696; DOI=10.1046/j.1365-313x.2003.01768.x;
RA Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J.,
RA Hellmann H., Estelle M., Somers D.E., Crosby W.L.;
RT "Protein interaction analysis of SCF ubiquitin E3 ligase subunits from
RT Arabidopsis.";
RL Plant J. 34:753-767(2003).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION,
RP INDUCTION, DOMAINS MD1 AND MD2, AND INTERACTION WITH SKP1A/ASK1;
RP SKP1B/ASK2; ASK4; ASK11 AND ASK14.
RX PubMed=17240087; DOI=10.1016/j.gene.2006.11.016;
RA Calderon-Villalobos L.I.A., Nill C., Marrocco K., Kretsch T.,
RA Schwechheimer C.;
RT "The evolutionarily conserved Arabidopsis thaliana F-box protein AtFBP7 is
RT required for efficient translation during temperature stress.";
RL Gene 392:106-116(2007).
CC -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC complexes, which may mediate the ubiquitination and subsequent
CC proteasomal degradation of target proteins (By similarity). Required
CC for efficient protein synthesis during temperature stress conditions.
CC {ECO:0000250, ECO:0000269|PubMed:17240087}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (ASK-cullin-F-box) protein ligase complex.
CC Interacts with SKP1A/ASK1, SKP1B/ASK2, ASK4, ASK11 and ASK14.
CC {ECO:0000269|PubMed:12169662, ECO:0000269|PubMed:12795696,
CC ECO:0000269|PubMed:17240087}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17240087}.
CC Mitochondrion {ECO:0000269|PubMed:17240087}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, flowers and stems, and, to a
CC lower extent, in roots and siliques. {ECO:0000269|PubMed:17240087}.
CC -!- DEVELOPMENTAL STAGE: In seedlings, mostly localized in shoot meristems,
CC root tips and hypocotyl. In adult plant, strongest levels observed in
CC the vasculature, pollen, pollen tubes, and trichomes. Also found in
CC senescencing leaves. {ECO:0000269|PubMed:17240087}.
CC -!- INDUCTION: By temperature stresses. {ECO:0000269|PubMed:17240087}.
CC -!- DOMAIN: The F-box is necessary for the interaction with ASK proteins.
CC {ECO:0000250}.
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DR EMBL; AC007727; AAD41431.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30153.1; -; Genomic_DNA.
DR EMBL; BT006427; AAP21235.1; -; mRNA.
DR EMBL; AK228033; BAE99994.1; -; mRNA.
DR PIR; C86351; C86351.
DR RefSeq; NP_564150.1; NM_102025.3.
DR AlphaFoldDB; Q9XI00; -.
DR BioGRID; 24019; 9.
DR IntAct; Q9XI00; 5.
DR STRING; 3702.AT1G21760.1; -.
DR PaxDb; Q9XI00; -.
DR PRIDE; Q9XI00; -.
DR EnsemblPlants; AT1G21760.1; AT1G21760.1; AT1G21760.
DR GeneID; 838780; -.
DR Gramene; AT1G21760.1; AT1G21760.1; AT1G21760.
DR KEGG; ath:AT1G21760; -.
DR Araport; AT1G21760; -.
DR TAIR; locus:2036932; AT1G21760.
DR eggNOG; KOG2997; Eukaryota.
DR HOGENOM; CLU_073443_0_0_1; -.
DR InParanoid; Q9XI00; -.
DR OrthoDB; 1407789at2759; -.
DR PhylomeDB; Q9XI00; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9XI00; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9XI00; baseline and differential.
DR Genevisible; Q9XI00; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0090406; C:pollen tube; IDA:TAIR.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0006417; P:regulation of translation; IMP:TAIR.
DR GO; GO:0009409; P:response to cold; IMP:TAIR.
DR GO; GO:0009408; P:response to heat; IMP:TAIR.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR045118; FBXO9/FBXO48.
DR InterPro; IPR045464; Hrt3/FBXO9_C.
DR PANTHER; PTHR12874; PTHR12874; 1.
DR Pfam; PF12937; F-box-like; 1.
DR Pfam; PF19270; FBO_C; 1.
DR SMART; SM00256; FBOX; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Mitochondrion; Nucleus; Reference proteome; Stress response;
KW Ubl conjugation pathway.
FT CHAIN 1..328
FT /note="F-box protein 7"
FT /id="PRO_0000272205"
FT DOMAIN 56..102
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REGION 126..158
FT /note="Mystery domain 1 (MD1)"
FT REGION 163..201
FT /note="Mystery domain 2 (MD2)"
SQ SEQUENCE 328 AA; 38043 MW; 4A6CD30291D78908 CRC64;
MTSDALTIPS ELESALRLRT VQYFITKRPW LDLYGVHVRP VPPFGSTSRK PHFDPALIHR
CLPDELLFEV FARMMPYDLG RASCVCRKWR YTVRNPMFWR NACLKAWQTA GVIENYKILQ
SKYDGSWRKM WLLRSRVRTD GLYVSRNTYI RAGIAEWKIT NPVHIVCYYR YIRFYPSGRF
LYKNSSQKLK DVAKYMNFKA SKSENLYKGT YTLSMSDDKI EAAVLYPGTR PTVLRIRLRL
RGTAIGANNR MDLLSLVTSG VNDEEISSTE EDILGLVEGW EDDETHNPDI PAVSHKRGMT
AFVFVPFEEV DESVLNLPPE KMDYYVTG
