SKIP5_ARATH
ID SKIP5_ARATH Reviewed; 274 AA.
AC Q94FT2; A0A1I9LNL9; Q3E7R9; Q9FPG4; Q9FV00; Q9FYV5; Q9M1H9;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=F-box protein SKIP5;
DE AltName: Full=SKP1-interacting partner 5;
GN Name=SKIP5; OrderedLocusNames=At3g54480; ORFNames=T14E10.50;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RA Kafer C.W., Thornburg R.W.;
RT "Transcription of the UMP synthase locus.";
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 14-274 (ISOFORM 1), AND INTERACTION WITH
RP SKP1A/ASK1.
RX PubMed=11387208; DOI=10.1093/emboj/20.11.2742;
RA Farras R., Ferrando A., Jasik J., Kleinow T., Oekresz L., Tiburcio A.,
RA Salchert K., del Pozo C., Schell J., Koncz C.;
RT "SKP1-SnRK protein kinase interactions mediate proteasomal binding of a
RT plant SCF ubiquitin ligase.";
RL EMBO J. 20:2742-2756(2001).
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 181-274 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Kafer C.W., Thornburg R.W.;
RT "Transcriptional analysis of the Arabidopsis thaliana UMP synthase locus.";
RL Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By
CC similarity). Interacts with SKP1A/ASK1. {ECO:0000250,
CC ECO:0000269|PubMed:11387208}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q94FT2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q94FT2-2; Sequence=VSP_022373, VSP_022374;
CC Name=3;
CC IsoId=Q94FT2-3; Sequence=VSP_022371, VSP_022372;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG21980.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAB77568.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF347971; AAK61344.1; -; mRNA.
DR EMBL; AF347972; AAK61345.1; -; mRNA.
DR EMBL; AL138656; CAB77568.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79236.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64173.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64176.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64177.1; -; Genomic_DNA.
DR EMBL; AF325053; AAG40405.1; -; mRNA.
DR EMBL; BT005863; AAO64798.1; -; mRNA.
DR EMBL; AK227454; BAE99457.1; -; mRNA.
DR EMBL; AY085042; AAM61599.1; -; mRNA.
DR EMBL; AF263381; AAG21980.1; ALT_INIT; mRNA.
DR EMBL; AF276887; AAK69441.1; -; Genomic_DNA.
DR EMBL; AF276888; AAG01324.1; -; mRNA.
DR PIR; T47607; T47607.
DR RefSeq; NP_001326219.1; NM_001339667.1. [Q94FT2-2]
DR RefSeq; NP_001326222.1; NM_001339669.1. [Q94FT2-1]
DR RefSeq; NP_001326223.1; NM_001339668.1. [Q94FT2-2]
DR RefSeq; NP_566999.1; NM_115304.3. [Q94FT2-1]
DR AlphaFoldDB; Q94FT2; -.
DR SMR; Q94FT2; -.
DR BioGRID; 9929; 7.
DR STRING; 3702.AT3G54480.1; -.
DR PaxDb; Q94FT2; -.
DR ProteomicsDB; 232593; -. [Q94FT2-1]
DR EnsemblPlants; AT3G54480.1; AT3G54480.1; AT3G54480. [Q94FT2-1]
DR EnsemblPlants; AT3G54480.3; AT3G54480.3; AT3G54480. [Q94FT2-2]
DR EnsemblPlants; AT3G54480.4; AT3G54480.4; AT3G54480. [Q94FT2-2]
DR EnsemblPlants; AT3G54480.5; AT3G54480.5; AT3G54480. [Q94FT2-1]
DR GeneID; 824613; -.
DR Gramene; AT3G54480.1; AT3G54480.1; AT3G54480. [Q94FT2-1]
DR Gramene; AT3G54480.3; AT3G54480.3; AT3G54480. [Q94FT2-2]
DR Gramene; AT3G54480.4; AT3G54480.4; AT3G54480. [Q94FT2-2]
DR Gramene; AT3G54480.5; AT3G54480.5; AT3G54480. [Q94FT2-1]
DR KEGG; ath:AT3G54480; -.
DR Araport; AT3G54480; -.
DR TAIR; locus:2096956; AT3G54480.
DR eggNOG; ENOG502QPSM; Eukaryota.
DR HOGENOM; CLU_082924_0_0_1; -.
DR InParanoid; Q94FT2; -.
DR OMA; FLSTCKV; -.
DR OrthoDB; 1067569at2759; -.
DR PhylomeDB; Q94FT2; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q94FT2; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q94FT2; baseline and differential.
DR Genevisible; Q94FT2; AT.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR InterPro; IPR045140; SHCBP1-like.
DR PANTHER; PTHR14695; PTHR14695; 2.
DR Pfam; PF12937; F-box-like; 1.
DR SMART; SM00256; FBOX; 1.
DR SUPFAM; SSF51126; SSF51126; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..274
FT /note="F-box protein SKIP5"
FT /id="PRO_0000272227"
FT DOMAIN 32..79
FT /note="F-box"
FT VAR_SEQ 105..118
FT /note="GDTILIVAGGNYRV -> VILSVTLFTLLCYS (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_022371"
FT VAR_SEQ 119..274
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_022372"
FT VAR_SEQ 149..156
FT /note="SALELLST -> RFGTHIPS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172, ECO:0000303|Ref.1"
FT /id="VSP_022373"
FT VAR_SEQ 157..274
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172, ECO:0000303|Ref.1"
FT /id="VSP_022374"
FT CONFLICT 198
FT /note="C -> Y (in Ref. 8; AAG01324/AAK69441)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 274 AA; 30556 MW; 8489E9F375817382 CRC64;
MELHEISKLD NKKHPERIVE SKMKKRMKPY SLTSLNNLDD GCLMHILSFL SPIPDRYNTA
LVCHRWRYLA CHPRLWLRVD RFVKDLSQPG VFLNIESAVS AARPGDTILI VAGGNYRVSN
IQIKKPLCLV GGGEIPDETT LVCARGSDSA LELLSTCKLA NLTVKAELGC CLLHRSGRLT
IDGCVLQCET NPLDHLSCPI VSTAGDEDIE NILSHVEVKE TVTGKIKANS VTVLQTRIEG
GAKAVSTRGD LVLQRVRVMY SKAYLYFWFD VDYE
