SKIT2_MOUSE
ID SKIT2_MOUSE Reviewed; 400 AA.
AC A7XUX6; A7TZE8; A7TZE9; A7XUY1;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Selection and upkeep of intraepithelial T-cells protein 2;
DE Short=Skint-2;
DE Flags: Precursor;
GN Name=Skint2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J, and FVB/NJ;
RX PubMed=18408721; DOI=10.1038/ng.108;
RA Boyden L.M., Lewis J.M., Barbee S.D., Bas A., Girardi M., Hayday A.C.,
RA Tigelaar R.E., Lifton R.P.;
RT "Skint1, the prototype of a newly identified immunoglobulin superfamily
RT gene cluster, positively selects epidermal gammadelta T cells.";
RL Nat. Genet. 40:656-662(2008).
CC -!- FUNCTION: May act by engaging a cell surface molecule on immature T-
CC cells in the embryonic thymus. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=A;
CC IsoId=A7XUX6-1; Sequence=Displayed;
CC Name=2; Synonyms=B;
CC IsoId=A7XUX6-2; Sequence=VSP_034877;
CC Name=3; Synonyms=C;
CC IsoId=A7XUX6-3; Sequence=VSP_034878, VSP_034879;
CC -!- TISSUE SPECIFICITY: Expressed in skin, thymus and mammary gland.
CC {ECO:0000269|PubMed:18408721}.
CC -!- MISCELLANEOUS: Encoded by one of the 11 copies of Skint genes clustered
CC in the D1 region of the chromosome 4.
CC -!- SIMILARITY: Belongs to the SKINT family. {ECO:0000305}.
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DR EMBL; EF494892; ABS30714.1; -; mRNA.
DR EMBL; EF494893; ABS30715.1; -; mRNA.
DR EMBL; EU099299; ABU87897.1; -; mRNA.
DR EMBL; EU099300; ABU87898.1; -; mRNA.
DR CCDS; CCDS38840.2; -. [A7XUX6-1]
DR RefSeq; NP_001272892.1; NM_001285963.1. [A7XUX6-1]
DR RefSeq; NP_001272894.1; NM_001285965.1. [A7XUX6-2]
DR AlphaFoldDB; A7XUX6; -.
DR SMR; A7XUX6; -.
DR STRING; 10090.ENSMUSP00000139831; -.
DR GlyGen; A7XUX6; 1 site.
DR jPOST; A7XUX6; -.
DR PaxDb; A7XUX6; -.
DR PRIDE; A7XUX6; -.
DR DNASU; 329919; -.
DR Ensembl; ENSMUST00000186969; ENSMUSP00000139831; ENSMUSG00000034359. [A7XUX6-1]
DR GeneID; 329919; -.
DR KEGG; mmu:329919; -.
DR UCSC; uc029uys.1; mouse. [A7XUX6-2]
DR UCSC; uc033idr.1; mouse. [A7XUX6-1]
DR CTD; 329919; -.
DR MGI; MGI:3649629; Skint2.
DR VEuPathDB; HostDB:ENSMUSG00000034359; -.
DR eggNOG; ENOG502SEQH; Eukaryota.
DR GeneTree; ENSGT00940000162562; -.
DR HOGENOM; CLU_013137_8_7_1; -.
DR InParanoid; A7XUX6; -.
DR OrthoDB; 812118at2759; -.
DR PhylomeDB; A7XUX6; -.
DR BioGRID-ORCS; 329919; 4 hits in 68 CRISPR screens.
DR PRO; PR:A7XUX6; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; A7XUX6; protein.
DR Bgee; ENSMUSG00000034359; Expressed in zone of skin and 10 other tissues.
DR ExpressionAtlas; A7XUX6; differential.
DR GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005102; F:signaling receptor binding; IBA:GO_Central.
DR GO; GO:0001817; P:regulation of cytokine production; IBA:GO_Central.
DR GO; GO:0050852; P:T cell receptor signaling pathway; IBA:GO_Central.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR013162; CD80_C2-set.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR Pfam; PF08205; C2-set_2; 1.
DR Pfam; PF07686; V-set; 1.
DR SMART; SM00409; IG; 1.
DR SMART; SM00406; IGv; 1.
DR SUPFAM; SSF48726; SSF48726; 2.
DR PROSITE; PS50835; IG_LIKE; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW Membrane; Reference proteome; Repeat; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..400
FT /note="Selection and upkeep of intraepithelial T-cells
FT protein 2"
FT /id="PRO_5000271635"
FT TOPO_DOM 22..240
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..280
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 281..301
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 302..321
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 322..342
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 343..400
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 23..133
FT /note="Ig-like V-type"
FT DOMAIN 139..225
FT /note="Ig-like C1-type"
FT CARBOHYD 197
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 46..120
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 160..214
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT VAR_SEQ 347..400
FT /note="DHQGWSLPPYLSATPTAAICRLAVPEYSRGHLQLDSEDDLAGMGPSPFFITP
FT CF -> LTAITRRSSGVESATLSFCHSHCCYLPPSCSRVFTWSPAAGLRR (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:18408721"
FT /id="VSP_034877"
FT VAR_SEQ 347..350
FT /note="DHQG -> LKSD (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:18408721"
FT /id="VSP_034878"
FT VAR_SEQ 351..400
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:18408721"
FT /id="VSP_034879"
FT CONFLICT 16
FT /note="Q -> H (in Ref. 1; ABS30715)"
FT /evidence="ECO:0000305"
FT CONFLICT 19
FT /note="T -> A (in Ref. 1; ABS30715)"
FT /evidence="ECO:0000305"
FT CONFLICT 201
FT /note="T -> M (in Ref. 1; ABS30715)"
FT /evidence="ECO:0000305"
FT CONFLICT 212
FT /note="I -> V (in Ref. 1; ABS30715)"
FT /evidence="ECO:0000305"
FT CONFLICT 223
FT /note="Q -> E (in Ref. 1; ABS30715)"
FT /evidence="ECO:0000305"
FT CONFLICT 226
FT /note="S -> R (in Ref. 1; ABS30715)"
FT /evidence="ECO:0000305"
FT CONFLICT 240
FT /note="R -> H (in Ref. 1; ABS30715)"
FT /evidence="ECO:0000305"
FT CONFLICT 270
FT /note="T -> S (in Ref. 1; ABS30715)"
FT /evidence="ECO:0000305"
FT CONFLICT 274
FT /note="G -> E (in Ref. 1; ABS30715)"
FT /evidence="ECO:0000305"
FT CONFLICT 284
FT /note="I -> V (in Ref. 1; ABS30715)"
FT /evidence="ECO:0000305"
FT CONFLICT 319
FT /note="L -> R (in Ref. 1; ABS30715)"
FT /evidence="ECO:0000305"
FT CONFLICT A7XUX6-3:350
FT /note="D -> G (in Ref. 1; ABS30715)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 400 AA; 45644 MW; 88A8FB358AF87389 CRC64;
MGATGVLLCV VLHFLQMVTQ SSEKFTVTGL QRPVLAPLGG NVELSCQLSP PQQAQHMEIR
WFRNRYREPV YLYRNGKDLH GETISKYVER TELLKDDIGK GKVTLRIFKL TADDDGSYHC
VFKVGEFYEE HITEIKVTAT SSVMYILMQP PNIKGVMLEC HSGGWFPQPH MEWRDNKGNI
IPATSKAHSQ DENKLFNMTM TLLIEASSHR SITCYLQNLL THQEESISIV LSGELFSWKR
VWIMILTTIG FMMIAFCMTY CVQQHLLYGT FSKGKCHWLK STMIFMFSVI AVTGVMLILH
LKQRVPVSDQ HFELDTLWLE DISVILCVLI VFIIKLISFI YFRLEGDHQG WSLPPYLSAT
PTAAICRLAV PEYSRGHLQL DSEDDLAGMG PSPFFITPCF
