SKIT3_MOUSE
ID SKIT3_MOUSE Reviewed; 458 AA.
AC A7TZF0; A7TZF2; Q8BIN0;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Selection and upkeep of intraepithelial T-cells protein 3;
DE Short=Skint-3;
DE Flags: Precursor;
GN Name=Skint3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J;
RX PubMed=18408721; DOI=10.1038/ng.108;
RA Boyden L.M., Lewis J.M., Barbee S.D., Bas A., Girardi M., Hayday A.C.,
RA Tigelaar R.E., Lifton R.P.;
RT "Skint1, the prototype of a newly identified immunoglobulin superfamily
RT gene cluster, positively selects epidermal gammadelta T cells.";
RL Nat. Genet. 40:656-662(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
CC -!- FUNCTION: May act by engaging a cell surface molecule on immature T-
CC cells in the embryonic thymus. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=A;
CC IsoId=A7TZF0-1; Sequence=Displayed;
CC Name=2; Synonyms=B;
CC IsoId=A7TZF0-2; Sequence=VSP_034881;
CC Name=3; Synonyms=C;
CC IsoId=A7TZF0-3; Sequence=VSP_034880;
CC -!- TISSUE SPECIFICITY: Expressed in skin and thymus.
CC {ECO:0000269|PubMed:18408721}.
CC -!- MISCELLANEOUS: Encoded by one of the 11 copies of Skint genes clustered
CC in the D1 region of the chromosome 4.
CC -!- SIMILARITY: Belongs to the SKINT family. {ECO:0000305}.
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DR EMBL; EF494894; ABS30716.1; -; mRNA.
DR EMBL; EF494895; ABS30717.1; -; mRNA.
DR EMBL; EF494896; ABS30718.1; -; mRNA.
DR EMBL; AK040383; BAC30578.1; -; mRNA.
DR CCDS; CCDS38838.1; -. [A7TZF0-2]
DR CCDS; CCDS51268.1; -. [A7TZF0-1]
DR RefSeq; NP_001095944.1; NM_001102474.2. [A7TZF0-1]
DR RefSeq; NP_001335283.1; NM_001348354.1. [A7TZF0-3]
DR RefSeq; NP_808246.1; NM_177578.4. [A7TZF0-2]
DR AlphaFoldDB; A7TZF0; -.
DR SMR; A7TZF0; -.
DR GlyGen; A7TZF0; 1 site.
DR PaxDb; A7TZF0; -.
DR PRIDE; A7TZF0; -.
DR ProteomicsDB; 261052; -. [A7TZF0-1]
DR ProteomicsDB; 261053; -. [A7TZF0-2]
DR DNASU; 195564; -.
DR Ensembl; ENSMUST00000038455; ENSMUSP00000042662; ENSMUSG00000070868. [A7TZF0-2]
DR Ensembl; ENSMUST00000170945; ENSMUSP00000131300; ENSMUSG00000070868. [A7TZF0-1]
DR GeneID; 195564; -.
DR KEGG; mmu:195564; -.
DR UCSC; uc008udt.1; mouse. [A7TZF0-1]
DR UCSC; uc008udu.1; mouse. [A7TZF0-2]
DR UCSC; uc012dir.1; mouse. [A7TZF0-3]
DR CTD; 195564; -.
DR MGI; MGI:3045331; Skint3.
DR VEuPathDB; HostDB:ENSMUSG00000070868; -.
DR GeneTree; ENSGT00940000162562; -.
DR HOGENOM; CLU_013137_8_7_1; -.
DR InParanoid; A7TZF0; -.
DR OMA; IVFSSMC; -.
DR OrthoDB; 1537230at2759; -.
DR PhylomeDB; A7TZF0; -.
DR TreeFam; TF331083; -.
DR BioGRID-ORCS; 195564; 1 hit in 69 CRISPR screens.
DR PRO; PR:A7TZF0; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; A7TZF0; protein.
DR Bgee; ENSMUSG00000070868; Expressed in zone of skin and 10 other tissues.
DR GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005102; F:signaling receptor binding; IBA:GO_Central.
DR GO; GO:0001817; P:regulation of cytokine production; IBA:GO_Central.
DR GO; GO:0050852; P:T cell receptor signaling pathway; IBA:GO_Central.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR013162; CD80_C2-set.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR Pfam; PF08205; C2-set_2; 1.
DR Pfam; PF07686; V-set; 1.
DR SMART; SM00409; IG; 1.
DR SMART; SM00406; IGv; 1.
DR SUPFAM; SSF48726; SSF48726; 2.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW Membrane; Reference proteome; Repeat; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..458
FT /note="Selection and upkeep of intraepithelial T-cells
FT protein 3"
FT /id="PRO_5000270105"
FT TOPO_DOM 25..237
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 259..283
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 305..324
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 325..345
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 346..359
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 360..380
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 381..414
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 415..435
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 436..458
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 26..141
FT /note="Ig-like V-type"
FT DOMAIN 142..231
FT /note="Ig-like C1-type"
FT CARBOHYD 200
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 49..123
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 163..217
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT VAR_SEQ 26..141
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:18408721"
FT /id="VSP_034880"
FT VAR_SEQ 236..307
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072,
FT ECO:0000303|PubMed:18408721"
FT /id="VSP_034881"
SQ SEQUENCE 458 AA; 53068 MW; 32B0B82C80459637 CRC64;
MGSIQIIFAA YCVVLCVLQM LVLSSEQFTI TGLERPVLAP LGGILELSCQ LSPPQNAQQM
EIRWFRNRYT EPVYLYRNGK DLHGETISKY VERTELLKHD IGKGKVTLRV FKVTVDDDGS
YHCVFKDGIF YEEHITEVKV TATSSDIKII MHPPNIKGVM LECHSRGWFP QPHMEWRDSN
GQVIPATSKS QSQDENKLFN MTMNLFADVG LHQIVTCYIQ NLLTHQEESI SIVLTGDLFS
WKIDWILILS IIACVMIPYS MTSYLQQHLI HGSCSQRSHH WRKNAMVCMS SVIAIIGSML
ILHLKQRVPI SDQHFELDTL YLEDISVILC VVIVFNLKLN LLTYYRLERK YDGCTPGCKA
CFYILKIIII ILPFVFTFGC YNAIFLKYHQ LQKKVSIPDP LYYFYTSWLV NMEMLGVFLV
FFPTFINLIE FSQFIKTVPK PIWLCQENMR EDDAIRHR
