SKIT8_MOUSE
ID SKIT8_MOUSE Reviewed; 398 AA.
AC A7XV07; A7TZF9; A7TZG0; A7XV10; E9QA97;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2013, sequence version 2.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Selection and upkeep of intraepithelial T-cells protein 8;
DE Short=Skint-8;
DE Flags: Precursor;
GN Name=Skint8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J, and FVB/NJ;
RX PubMed=18408721; DOI=10.1038/ng.108;
RA Boyden L.M., Lewis J.M., Barbee S.D., Bas A., Girardi M., Hayday A.C.,
RA Tigelaar R.E., Lifton R.P.;
RT "Skint1, the prototype of a newly identified immunoglobulin superfamily
RT gene cluster, positively selects epidermal gammadelta T cells.";
RL Nat. Genet. 40:656-662(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: May act by engaging a cell surface molecule on immature T-
CC cells in the embryonic thymus. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=A;
CC IsoId=A7XV07-1; Sequence=Displayed;
CC Name=2; Synonyms=B;
CC IsoId=A7XV07-2; Sequence=VSP_034894, VSP_034895;
CC Name=3; Synonyms=C;
CC IsoId=A7XV07-3; Sequence=VSP_034892, VSP_034893;
CC -!- TISSUE SPECIFICITY: Expressed in skin, thymus, testis and, to a lower
CC extent, bladder, brain, heart, kidney, mammary gland, small intestine
CC and uterus. {ECO:0000269|PubMed:18408721}.
CC -!- MISCELLANEOUS: Encoded by one of the 11 copies of Skint genes clustered
CC in the D1 region of the chromosome 4.
CC -!- SIMILARITY: Belongs to the SKINT family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABS30725.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=ABS30726.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=ABU87905.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=ABU87906.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; EF494903; ABS30725.1; ALT_INIT; mRNA.
DR EMBL; EF494904; ABS30726.1; ALT_INIT; mRNA.
DR EMBL; EU099307; ABU87905.1; ALT_INIT; mRNA.
DR EMBL; EU099308; ABU87906.1; ALT_INIT; mRNA.
DR EMBL; AL627076; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS51265.1; -. [A7XV07-1]
DR RefSeq; NP_001093936.2; NM_001100466.1. [A7XV07-1]
DR RefSeq; XP_017175827.1; XM_017320338.1. [A7XV07-1]
DR AlphaFoldDB; A7XV07; -.
DR SMR; A7XV07; -.
DR GlyGen; A7XV07; 2 sites.
DR PaxDb; A7XV07; -.
DR PRIDE; A7XV07; -.
DR ProteomicsDB; 261059; -. [A7XV07-3]
DR Ensembl; ENSMUST00000165046; ENSMUSP00000133268; ENSMUSG00000078599. [A7XV07-1]
DR GeneID; 639774; -.
DR KEGG; mmu:639774; -.
DR UCSC; uc008udl.1; mouse. [A7XV07-2]
DR UCSC; uc009vct.1; mouse. [A7XV07-3]
DR UCSC; uc009vcu.1; mouse. [A7XV07-1]
DR CTD; 639774; -.
DR MGI; MGI:3651523; Skint8.
DR VEuPathDB; HostDB:ENSMUSG00000078599; -.
DR eggNOG; ENOG502TCM5; Eukaryota.
DR GeneTree; ENSGT00940000164707; -.
DR InParanoid; A7XV07; -.
DR OMA; FLTICFV; -.
DR OrthoDB; 814950at2759; -.
DR PhylomeDB; A7XV07; -.
DR TreeFam; TF331083; -.
DR BioGRID-ORCS; 639774; 2 hits in 72 CRISPR screens.
DR PRO; PR:A7XV07; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; A7XV07; protein.
DR Bgee; ENSMUSG00000078599; Expressed in zone of skin and 8 other tissues.
DR ExpressionAtlas; A7XV07; baseline and differential.
DR GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005102; F:signaling receptor binding; IBA:GO_Central.
DR GO; GO:0001817; P:regulation of cytokine production; IBA:GO_Central.
DR GO; GO:0050852; P:T cell receptor signaling pathway; IBA:GO_Central.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR Pfam; PF07686; V-set; 1.
DR SMART; SM00409; IG; 1.
DR SUPFAM; SSF48726; SSF48726; 2.
DR PROSITE; PS50835; IG_LIKE; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW Membrane; Reference proteome; Repeat; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..398
FT /note="Selection and upkeep of intraepithelial T-cells
FT protein 8"
FT /id="PRO_5000271643"
FT TOPO_DOM 26..244
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 266..288
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 310..331
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 353..398
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 26..141
FT /note="Ig-like V-type"
FT DOMAIN 142..233
FT /note="Ig-like C1-type"
FT CARBOHYD 92
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 139
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 49..123
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 163..217
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT VAR_SEQ 142..154
FT /note="AVGLETEIHVQAP -> GKLFVEHSIPVTL (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:18408721"
FT /id="VSP_034892"
FT VAR_SEQ 155..398
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:18408721"
FT /id="VSP_034893"
FT VAR_SEQ 236..256
FT /note="NELFNQDYLWVGIFPFSVLSL -> SEYCHLSWLLISKQREKYGGN (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:18408721"
FT /id="VSP_034894"
FT VAR_SEQ 257..398
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:18408721"
FT /id="VSP_034895"
FT CONFLICT 36
FT /note="H -> Y (in Ref. 1; ABS30726)"
FT /evidence="ECO:0000305"
FT CONFLICT 46
FT /note="E -> V (in Ref. 1; ABS30726)"
FT /evidence="ECO:0000305"
FT CONFLICT 225
FT /note="G -> L (in Ref. 1; ABU87906)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 398 AA; 45092 MW; B2841AD91D4192E7 CRC64;
MMKPEFSHFF GFCVYFLFLQ VMASSEKLRV TTPTRHLLAR VGGQAELSCQ VIPPHSVMHM
EVRWFRSGHS QPVYLYRGGH KMSEEAAPEY ANRTEFVKEA IGEGKVSLRI YNINILDDGP
YQCSFNDSGF IDVAIMNLNV TAVGLETEIH VQAPDADGVM VECNTGGWFP RPQMEWRDSK
GATLPHSLKS YSQDEARFFH MKMTLLLTNM SHGSIICCIS NPVTGEEKQT SIILANELFN
QDYLWVGIFP FSVLSLILFG VLPFINSFFR SQGCASGCLS KCLPVVTSWP VQIVHFLVCS
GVLFAVYLPH RYRVSLSDPQ FPLYNNWITE LLIVILFLTI CFVLPITVLL LIKLSPTCLA
KWEKNKDDIM DSQLGLGKAR EASTLYEEQS RKSWEQEK
