SKP1_MACFA
ID SKP1_MACFA Reviewed; 163 AA.
AC Q4R5B9; Q4R644;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=S-phase kinase-associated protein 1;
DE AltName: Full=Cyclin-A/CDK2-associated protein p19;
DE AltName: Full=S-phase kinase-associated protein 1A;
DE AltName: Full=p19A;
DE AltName: Full=p19skp1;
GN Name=SKP1; Synonyms=SKP1A; ORFNames=QtrA-12126, QtsA-19170;
OS Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Cercopithecidae; Cercopithecinae; Macaca.
OX NCBI_TaxID=9541;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Temporal cortex, and Testis;
RG International consortium for macaque cDNA sequencing and analysis;
RT "DNA sequences of macaque genes expressed in brain or testis and its
RT evolutionary implications.";
RL Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Essential component of the SCF (SKP1-CUL1-F-box protein)
CC ubiquitin ligase complex, which mediates the ubiquitination of proteins
CC involved in cell cycle progression, signal transduction and
CC transcription. In the SCF complex, serves as an adapter that links the
CC F-box protein to CUL1. The functional specificity of the SCF complex
CC depends on the F-box protein as substrate recognition component.
CC SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and
CC participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of
CC phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB,
CC NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2.
CC SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is
CC involved in regulation of G1/S transition. SCF(SKP2) directs
CC ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and
CC probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E,
CC NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
CC SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs
CC ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and
CC DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs
CC ubiquitination of BCL6 and DTL but does not seem to direct
CC ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA
CC at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-
CC RELA dimer to translocate into the nucleus and to activate
CC transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3)
CC and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9)
CC directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs
CC ubiquitination of BCL2. {ECO:0000250|UniProtKB:P63208}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Interacts with KDM2B, forming heterodimers (By similarity).
CC The KDM2B-SKP1 heterodimeric complex interacts with the PCGF1-BCORL
CC heterodimeric complex to form a homotetrameric polycomb repression
CC complex 1 (PRC1.1) (By similarity). Component of multiple SCF (SKP1-
CC CUL1-F-box) E3 ubiquitin-protein ligase complexes formed of CUL1, SKP1,
CC RBX1 and a variable F-box domain-containing protein as substrate-
CC specific subunit. Component of the SCF(FBXW11) complex containing
CC FBXW11. Component of the SCF(SKP2) complex containing SKP2, in which it
CC interacts directly with SKP1, SKP2 and RBX1. Component of the
CC SCF(FBXW2) complex containing FBXw2. Component of the SCF(FBXO32)
CC complex containing FBXO32. Component of the probable SCF(FBXO7) complex
CC containing FBXO7. Component of the SCF(FBXO10) complex containing
CC FBXO10. Component of the SCF(FBXO11) complex containing FBXO11.
CC Component of the SCF(FBXO25) complex containing FBXO25. Component of
CC the SCF(FBXO33) complex containing FBXO33. Component of the probable
CC SCF(FBXO4) complex containing FBXO4. Component of the SCF(FBXO44)
CC complex, composed of SKP1, CUL1 and FBXO44. Component of the SCF(BTRC)
CC complex, composed of SKP1, CUL1 and BTRC. This complex binds
CC phosphorylated NFKBIA. Part of a SCF complex consisting of CUL1, RBX1,
CC SKP1 and FBXO2. Component of a SCF(SKP2)-like complex containing CUL1,
CC SKP1, TRIM21 and SKP2. Component of the SCF(FBXO17) complex, composed
CC of SKP1, CUL1 and FBXO17. Component of the SCF(FBXO27) complex,
CC composed of SKP1, CUL1 and FBXO27. Component of the SCF(CCNF) complex
CC consisting of CUL1, RBX1, SKP1 and CCNF. Component of the SCF(FBXL3)
CC complex composed of CUL1, SKP1, RBX1 and FBXL3. Component of the
CC SCF(FBXL21) complex composed of CUL1, SKP1, RBX1 and FBXL21. Component
CC of the SCF(FBXO9) composed of CUL1, SKP1, RBX1 and FBXO9. Component of
CC the SCF(FBXW7) composed of CUL1, SKP1, RBX1 and FBXW7. Interacts with
CC CEP68 (By similarity). Interacts with FBXW15 and NOTHC2 (By
CC similarity). The SKP1-KDM2A and SKP1-KDM2B complexes interact with UBB
CC (By similarity). {ECO:0000250|UniProtKB:P63208,
CC ECO:0000250|UniProtKB:Q9WTX5}.
CC -!- PTM: Undergoes autophagy-mediated degradation in the liver in a time-
CC dependent manner. {ECO:0000250|UniProtKB:Q9WTX5}.
CC -!- SIMILARITY: Belongs to the SKP1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE01431.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB169346; BAE01431.1; ALT_FRAME; mRNA.
DR EMBL; AB169625; BAE01706.1; -; mRNA.
DR AlphaFoldDB; Q4R5B9; -.
DR SMR; Q4R5B9; -.
DR STRING; 9541.XP_005557832.1; -.
DR Ensembl; ENSMFAT00000090014; ENSMFAP00000054831; ENSMFAG00000052365.
DR eggNOG; KOG1724; Eukaryota.
DR GeneTree; ENSGT00390000012652; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000233100; Chromosome 1.
DR GO; GO:0031467; C:Cul7-RING ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0031519; C:PcG protein complex; IEA:Ensembl.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0008013; F:beta-catenin binding; IEA:Ensembl.
DR GO; GO:0097602; F:cullin family protein binding; IEA:Ensembl.
DR GO; GO:1990444; F:F-box domain binding; IEA:Ensembl.
DR GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; IEA:Ensembl.
DR GO; GO:0035518; P:histone H2A monoubiquitination; IEA:Ensembl.
DR GO; GO:0051457; P:maintenance of protein location in nucleus; IEA:Ensembl.
DR GO; GO:1904668; P:positive regulation of ubiquitin protein ligase activity; IEA:Ensembl.
DR GO; GO:0000209; P:protein polyubiquitination; IEA:Ensembl.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR016897; SKP1.
DR InterPro; IPR001232; SKP1-like.
DR InterPro; IPR036296; SKP1-like_dim_sf.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR InterPro; IPR016072; Skp1_comp_dimer.
DR InterPro; IPR016073; Skp1_comp_POZ.
DR PANTHER; PTHR11165; PTHR11165; 1.
DR Pfam; PF01466; Skp1; 1.
DR Pfam; PF03931; Skp1_POZ; 1.
DR PIRSF; PIRSF028729; E3_ubiquit_lig_SCF_Skp; 1.
DR SMART; SM00512; Skp1; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR SUPFAM; SSF81382; SSF81382; 1.
PE 2: Evidence at transcript level;
KW Isopeptide bond; Phosphoprotein; Reference proteome; Ubl conjugation;
KW Ubl conjugation pathway.
FT CHAIN 1..163
FT /note="S-phase kinase-associated protein 1"
FT /id="PRO_0000288491"
FT REGION 63..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 104..163
FT /note="Interaction with the F-box domain of F-box proteins"
FT /evidence="ECO:0000250"
FT MOD_RES 131
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P63208"
FT CROSSLNK 142
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO1)"
FT /evidence="ECO:0000250|UniProtKB:P63208"
SQ SEQUENCE 163 AA; 18658 MW; C794D62AFB75528A CRC64;
MPSIKLQSSD GEIFEVDVEI AKQSVTIKTM LEDLGMDDEG DDDPVPLPNV NAAILKKVIQ
WCTHHKDDPP PPEDDENKEK RTDDIPVWDQ EFLKVDQGTL FELILAANYL DIKGLLDVTC
KTVANMIKGK TPEEIRKTFN IKNDFTEEEE AQVRKENQWC EEK
