SKP2B_ARATH
ID SKP2B_ARATH Reviewed; 360 AA.
AC O49286; Q680E2; Q8GY48;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=F-box protein SKP2B;
DE AltName: Full=F-box/LRR-repeat protein 5;
DE Short=AtFB5;
DE AltName: Full=SKP2-like protein 2;
DE Short=AtSKP2;2;
GN Name=SKP2B; Synonyms=FBL5; OrderedLocusNames=At1g77000; ORFNames=F22K20.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 192-360.
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 192-360.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11077244; DOI=10.1016/s1360-1385(00)01769-6;
RA Xiao W., Jang J.-C.;
RT "F-box proteins in Arabidopsis.";
RL Trends Plant Sci. 5:454-457(2000).
RN [8]
RP FUNCTION.
RX PubMed=18005227; DOI=10.1111/j.1365-313x.2007.03370.x;
RA Ren H., Santner A., del Pozo J.C., Murray J.A., Estelle M.;
RT "Degradation of the cyclin-dependent kinase inhibitor KRP1 is regulated by
RT two different ubiquitin E3 ligases.";
RL Plant J. 53:705-716(2008).
RN [9]
RP FUNCTION, AND MUTAGENESIS OF SER-128.
RX PubMed=21139066; DOI=10.1105/tpc.110.078972;
RA Jurado S., Abraham Z., Manzano C., Lopez-Torrejon G., Pacios L.F.,
RA Del Pozo J.C.;
RT "The Arabidopsis cell cycle F-Box protein SKP2A binds to auxin.";
RL Plant Cell 22:3891-3904(2010).
CC -!- FUNCTION: Component of SCF(SKP2B) E3 ubiquitin ligase complexes, which
CC mediate the ubiquitination and subsequent proteasomal degradation of
CC the cyclin-dependent kinase inhibitor KRP1. Does not interact with
CC auxin. {ECO:0000269|PubMed:18005227, ECO:0000269|PubMed:21139066}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC42507.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC002291; AAC00619.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35923.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35924.1; -; Genomic_DNA.
DR EMBL; BT024747; ABD59085.1; -; mRNA.
DR EMBL; AY087441; AAM64987.1; -; mRNA.
DR EMBL; AK117866; BAC42507.1; ALT_INIT; mRNA.
DR EMBL; AK175607; BAD43370.1; -; mRNA.
DR EMBL; AK175925; BAD43688.1; -; mRNA.
DR PIR; A96799; A96799.
DR RefSeq; NP_001185415.1; NM_001198486.2.
DR RefSeq; NP_565147.1; NM_106351.4.
DR AlphaFoldDB; O49286; -.
DR SMR; O49286; -.
DR BioGRID; 29255; 5.
DR STRING; 3702.AT1G77000.2; -.
DR PaxDb; O49286; -.
DR PRIDE; O49286; -.
DR ProteomicsDB; 232638; -.
DR EnsemblPlants; AT1G77000.1; AT1G77000.1; AT1G77000.
DR EnsemblPlants; AT1G77000.2; AT1G77000.2; AT1G77000.
DR GeneID; 844036; -.
DR Gramene; AT1G77000.1; AT1G77000.1; AT1G77000.
DR Gramene; AT1G77000.2; AT1G77000.2; AT1G77000.
DR KEGG; ath:AT1G77000; -.
DR Araport; AT1G77000; -.
DR TAIR; locus:2025222; AT1G77000.
DR eggNOG; KOG1947; Eukaryota.
DR HOGENOM; CLU_032606_0_0_1; -.
DR InParanoid; O49286; -.
DR OMA; GNMITEW; -.
DR OrthoDB; 1046098at2759; -.
DR PhylomeDB; O49286; -.
DR PRO; PR:O49286; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O49286; baseline and differential.
DR Genevisible; O49286; AT.
DR GO; GO:0019005; C:SCF ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0010286; P:heat acclimation; IEP:TAIR.
DR GO; GO:1901332; P:negative regulation of lateral root development; IMP:TAIR.
DR GO; GO:0051603; P:proteolysis involved in protein catabolic process; IMP:TAIR.
DR GO; GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR006553; Leu-rich_rpt_Cys-con_subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF00646; F-box; 1.
DR Pfam; PF13516; LRR_6; 3.
DR SMART; SM00367; LRR_CC; 9.
DR SUPFAM; SSF81383; SSF81383; 1.
PE 1: Evidence at protein level;
KW Leucine-rich repeat; Reference proteome; Repeat.
FT CHAIN 1..360
FT /note="F-box protein SKP2B"
FT /id="PRO_0000272248"
FT DOMAIN 25..76
FT /note="F-box"
FT REPEAT 77..99
FT /note="LRR 1"
FT REPEAT 101..126
FT /note="LRR 2"
FT REPEAT 127..152
FT /note="LRR 3"
FT REPEAT 153..178
FT /note="LRR 4"
FT REPEAT 180..205
FT /note="LRR 5"
FT REPEAT 206..231
FT /note="LRR 6"
FT REPEAT 232..257
FT /note="LRR 7"
FT REPEAT 258..301
FT /note="LRR 8"
FT REPEAT 302..333
FT /note="LRR 9"
FT MUTAGEN 128
FT /note="S->L: Binds auxin."
FT /evidence="ECO:0000269|PubMed:21139066"
SQ SEQUENCE 360 AA; 39909 MW; 8F5D48664D5AB5B5 CRC64;
MVSEGATRKE LNLCFENMKM EGVLISEWKD IPVELLMKIL NLVDDRTVII ASCICSGWRD
AVSLGLTRLS LSWCKKNMNS LVLSLAPKFV KLQTLVLRQD KPQLEDNAVE AIANHCHELQ
DLDLSKSSKI TDHSLYSLAR GCTNLTKLNL SGCTSFSDTA LAHLTRFCRK LKILNLCGCV
EAVSDNTLQA IGENCNQLQS LNLGWCENIS DDGVMSLAYG CPDLRTLDLC SCVLITDESV
VALANRCIHL RSLGLYYCRN ITDRAMYSLA QSGVKNKHEM WRAVKKGKFD EEGLRSLNIS
QCTYLTPSAV QAVCDTFPAL HTCSGRHSLV MSGCLNLQSV HCACILQAHR THTVYPHPAH
