BHL42_ACTCC
ID BHL42_ACTCC Reviewed; 689 AA.
AC A0A2R6QE26; A0A410R938;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2018, sequence version 1.
DT 25-MAY-2022, entry version 14.
DE RecName: Full=Transcription factor BHLH42 {ECO:0000305};
DE AltName: Full=Basic helix-loop-helix protein 42 {ECO:0000305};
DE Short=AcBHLH42 {ECO:0000303|PubMed:30912865};
GN Name=BHLH42 {ECO:0000303|PubMed:30912865};
GN ORFNames=CEY00_Acc19563 {ECO:0000312|EMBL:PSS06386.1};
OS Actinidia chinensis var. chinensis (Chinese soft-hair kiwi).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; Ericales; Actinidiaceae; Actinidia.
OX NCBI_TaxID=1590841;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH MYB123, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=30912865; DOI=10.1111/tpj.14330;
RA Wang L., Tang W., Hu Y., Zhang Y., Sun J., Guo X., Lu H., Yang Y., Fang C.,
RA Niu X., Yue J., Fei Z., Liu Y.;
RT "A MYB/bHLH complex regulates tissue-specific anthocyanin biosynthesis in
RT the inner pericarp of red-centered kiwifruit Actinidia chinensis cv.
RT Hongyang.";
RL Plant J. 99:359-378(2019).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Red5;
RX PubMed=29661190; DOI=10.1186/s12864-018-4656-3;
RA Pilkington S.M., Crowhurst R., Hilario E., Nardozza S., Fraser L., Peng Y.,
RA Gunaseelan K., Simpson R., Tahir J., Deroles S.C., Templeton K., Luo Z.,
RA Davy M., Cheng C., McNeilage M., Scaglione D., Liu Y., Zhang Q., Datson P.,
RA De Silva N., Gardiner S.E., Bassett H., Chagne D., McCallum J.,
RA Dzierzon H., Deng C., Wang Y.Y., Barron L., Manako K., Bowen J.,
RA Foster T.M., Erridge Z.A., Tiffin H., Waite C.N., Davies K.M.,
RA Grierson E.P., Laing W.A., Kirk R., Chen X., Wood M., Montefiori M.,
RA Brummell D.A., Schwinn K.E., Catanach A., Fullerton C., Li D.,
RA Meiyalaghan S., Nieuwenhuizen N., Read N., Prakash R., Hunter D., Zhang H.,
RA McKenzie M., Knabel M., Harris A., Allan A.C., Gleave A., Chen A.,
RA Janssen B.J., Plunkett B., Ampomah-Dwamena C., Voogd C., Leif D.,
RA Lafferty D., Souleyre E.J.F., Varkonyi-Gasic E., Gambi F., Hanley J.,
RA Yao J.L., Cheung J., David K.M., Warren B., Marsh K., Snowden K.C.,
RA Lin-Wang K., Brian L., Martinez-Sanchez M., Wang M., Ileperuma N.,
RA Macnee N., Campin R., McAtee P., Drummond R.S.M., Espley R.V.,
RA Ireland H.S., Wu R., Atkinson R.G., Karunairetnam S., Bulley S.,
RA Chunkath S., Hanley Z., Storey R., Thrimawithana A.H., Thomson S.,
RA David C., Testolin R., Huang H., Hellens R.P., Schaffer R.J.;
RT "A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome
RT highlights the challenges associated with draft genomes and gene prediction
RT in plants.";
RL BMC Genomics 19:257-257(2018).
CC -!- FUNCTION: Transcription activator involved in the spatiotemporal
CC regulation of anthocyanin biosynthesis specifically in the inner
CC pericarp of red-fleshed kiwifruits (PubMed:30912865). Functions in
CC association with MYB123 to activate the promoters of LDOX (ANS) and
CC F3GT1 that encode the dedicated enzymes for anthocyanin biosynthesis
CC (PubMed:30912865). {ECO:0000269|PubMed:30912865}.
CC -!- SUBUNIT: Interacts with MYB123. {ECO:0000269|PubMed:30912865}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:30912865}.
CC -!- TISSUE SPECIFICITY: Expressed in the inner pericarp of maturing fruits.
CC {ECO:0000269|PubMed:30912865}.
CC -!- SIMILARITY: Belongs to the bHLH protein family.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MH643776; QAT77714.1; -; mRNA.
DR EMBL; NKQK01000017; PSS06386.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2R6QE26; -.
DR SMR; A0A2R6QE26; -.
DR STRING; 1590841.A0A2R6QE26; -.
DR EnsemblPlants; PSS06386; PSS06386; CEY00_Acc19563.
DR Gramene; PSS06386; PSS06386; CEY00_Acc19563.
DR OMA; VCIPVVD; -.
DR Proteomes; UP000241394; Chromosome lg17.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0031542; P:positive regulation of anthocyanin biosynthetic process; IDA:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR025610; MYC/MYB_N.
DR Pfam; PF14215; bHLH-MYC_N; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..689
FT /note="Transcription factor BHLH42"
FT /id="PRO_0000448076"
FT DOMAIN 483..532
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 192..287
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 458..489
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 483..496
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 497..532
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 547..570
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 247..271
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 461..480
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 87
FT /note="Q -> P (in Ref. 1; QAT77714)"
FT /evidence="ECO:0000305"
FT CONFLICT 256
FT /note="E -> D (in Ref. 1; QAT77714)"
FT /evidence="ECO:0000305"
FT CONFLICT 355
FT /note="P -> A (in Ref. 1; QAT77714)"
FT /evidence="ECO:0000305"
FT CONFLICT 376
FT /note="E -> D (in Ref. 1; QAT77714)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 689 AA; 76277 MW; 3A8F10513F191041 CRC64;
MAAPPSTRLQ GMLQTAVQSV QWTYSLFWQL CPQQGILVWG DGYYNGAIKT RKTVLPIEVS
AEEASLQRSQ QLRELYDSLS AGESNQQTRR PCAALSPEDL TESEWFYLMC VSFSFPPGVG
LPGKAYAKRQ HVWLTGANEV DSKVFSRAIL AKSARVQTVV CIPLLDGVVE LGNTERVQED
IGFIHQVKSF FIDHHPPPPP KPALSEHSTS NLATSSVHPR FHSPPLPLTY APLDPPLIAN
QIHMEEEQEQ EQEEDEDDDD DDDDEEEAES DSEAHTGLNS EAQNPRVDHV ARAAAEPSEL
MQLEMSEDIR LGSPDDGSNN MDPDFQLMVA SQGGNPADQQ QRVDSFRAES TRRWPLLQDP
LGRSLQAPTS GGTGLEELTQ DDTHYSQTVS TILQHQSNRW LESSSSSAAG CLMYSSQSSF
SKWPLRPSDH HHQADATSQC LLKYILFTVP FLHSKYRDDN NSPKSATAAD SASRFRKPTP
QDELSANHVL AERRRREKLN ERFIILRSLV PFVTKMDKAS ILGDTIEYVK QLRKNIQDLE
ARARQIEIDQ RSRSSGDPQR SGAKAATDKR KLRIVEASGG AKGKAVDSVA VATPPPPAPP
QPVAGVGVQV QVSIIESDAL VELQCTHREG LLLDVMVVLR DHRVEVTAVQ SSLTNGVFVA
ELRAKVKENV NGKKPSIVEV KRAIHQIIP
