SL11_ORYSI
ID SL11_ORYSI Reviewed; 694 AA.
AC A2ZLU6; Q64HA9;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 25-MAY-2022, entry version 75.
DE RecName: Full=Protein spotted leaf 11;
DE EC=2.3.2.27;
DE AltName: Full=Cell death-related protein SPL11;
DE AltName: Full=RING-type E3 ubiquitin transferase SPL11 {ECO:0000305};
GN Name=SPL11; ORFNames=OsI_037539;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [2]
RP DISRUPTION PHENOTYPE.
RC STRAIN=cv. IR68;
RX PubMed=10939258; DOI=10.1094/mpmi.2000.13.8.869;
RA Yin Z., Chen J., Zeng L., Goh M., Leung H., Khush G.S., Wang G.-L.;
RT "Characterizing rice lesion mimic mutants and identifying a mutant with
RT broad-spectrum resistance to rice blast and bacterial blight.";
RL Mol. Plant Microbe Interact. 13:869-876(2000).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS OF VAL-290.
RC STRAIN=cv. IR64, and cv. IR68;
RX PubMed=15377756; DOI=10.1105/tpc.104.025171;
RA Zeng L.-R., Qu S., Bordeos A., Yang C., Baraoidan M., Yan H., Xie Q.,
RA Nahm B.H., Leung H., Wang G.-L.;
RT "Spotted leaf11, a negative regulator of plant cell death and defense,
RT encodes a U-Box/Armadillo repeat protein endowed with E3 Ubiquitin ligase
RT activity.";
RL Plant Cell 16:2795-2808(2004).
CC -!- FUNCTION: Defense related protein that negatively regulates programmed
CC cell death. In vitro, possesses E3 ubiquitin ligase activity.
CC {ECO:0000269|PubMed:15377756}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- TISSUE SPECIFICITY: Highly expressed in leaf, at intermediate levels in
CC shoot and weakly in root. {ECO:0000269|PubMed:15377756}.
CC -!- INDUCTION: In both incompatible and compatible interactions with rice
CC blast fungus (M.grisea).
CC -!- DISRUPTION PHENOTYPE: Plants exhibit non-race-specific resistance to
CC rice blast fungus (M.grisea) and to bacterial blight (X.oryzae pv
CC oryzae). Lesion mimic mutant spl11 is expressing defense-related genes
CC in fully expanded leaf. {ECO:0000269|PubMed:10939258}.
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DR EMBL; CM000137; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; A2ZLU6; -.
DR SMR; A2ZLU6; -.
DR STRING; 39946.A2ZLU6; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000007015; Chromosome 12.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.20.930.20; -; 1.
DR Gene3D; 1.25.10.10; -; 2.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR036537; Adaptor_Cbl_N_dom_sf.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR Pfam; PF00514; Arm; 3.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 5.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50176; ARM_REPEAT; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Repeat; Transferase; Ubl conjugation pathway.
FT CHAIN 1..694
FT /note="Protein spotted leaf 11"
FT /id="PRO_0000300254"
FT DOMAIN 272..346
FT /note="U-box"
FT REPEAT 398..438
FT /note="ARM 1"
FT REPEAT 439..479
FT /note="ARM 2"
FT REPEAT 480..520
FT /note="ARM 3"
FT REPEAT 521..561
FT /note="ARM 4"
FT REPEAT 562..602
FT /note="ARM 5"
FT REPEAT 603..650
FT /note="ARM 6"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 343..363
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 650..694
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 650..671
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 290
FT /note="V->R: Loss of E3 ubiquitin ligase activity."
FT /evidence="ECO:0000269|PubMed:15377756"
SQ SEQUENCE 694 AA; 75300 MW; 3FEDB892C7289E05 CRC64;
MAGDRAEEEE GEAPPPEARA AAAVERVAAA VEAVAAGAGA GAGEYRNAYR RQLLALSRRI
RLLGPFVEEL RERRRGEGEG EEEERALAPL ADALEAALAL LRLGREGSRI SLVLERDSVM
KKFQGVILQL EQALCDIPYN ELDISDEVRE QVELVHAQLK RAKERIDMPD DEFYNDLLSV
YDKNYDPSAE LAILGRLSEK LHLMTITDLT QESLALHEMV ASGGGQDPGE HIERMSMLLK
KIKDFVQTQN PDMGPPMASR VLDSNGDSRP ITIPDEFRCP ISLELMKDPV IVSTGQTYER
ACIEKWIASG HHTCPTTQQK MSTSALTPNY VLRSLISQWC ETNGMEPPKR STQPNKPTPA
CSSSERANID ALLSKLCSPD TEEQRSAAAE LRLLAKRNAN NRICIAEAGA IPLLLSLLSS
SDLRTQEHAV TALLNLSIHE DNKASIISSG AVPSIVHVLK NGSMEARENA AATLFSLSVI
DEYKVTIGGM GAIPALVVLL GEGSQRGKKD AAAALFNLCI YQGNKGRAIR AGLVPLIMGL
VTNPTGALMD EAMAILSILS SHPEGKAAIG AAEPVPVLVE MIGSGTPRNR ENAAAVMLHL
CSGEHHLVHL ARAQECGIMV PLRELALNGT DRGKRKAVQL LERMSRFLVQ QQEEQESQSQ
ASAQVPPQAT PEQVPENDIP EQLDSPASQY PMVV
