SLA2_SCHPO
ID SLA2_SCHPO Reviewed; 1102 AA.
AC Q9P6L5; P78916;
DT 23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2012, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Endocytosis protein end4;
DE AltName: Full=SLA2 protein homolog;
GN Name=end4; Synonyms=sla2; ORFNames=SPAC688.11;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 635-1102.
RC STRAIN=PR745;
RX PubMed=9501991; DOI=10.1093/dnares/4.6.363;
RA Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.;
RT "Identification of open reading frames in Schizosaccharomyces pombe
RT cDNAs.";
RL DNA Res. 4:363-369(1997).
RN [4]
RP FUNCTION.
RX PubMed=15300681; DOI=10.1002/yea.1134;
RA Iwaki T., Tanaka N., Takagi H., Giga-Hama Y., Takegawa K.;
RT "Characterization of end4+, a gene required for endocytosis in
RT Schizosaccharomyces pombe.";
RL Yeast 21:867-881(2004).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15827087; DOI=10.1242/jcs.02311;
RA Castagnetti S., Behrens R., Nurse P.;
RT "End4/Sla2 is involved in establishment of a new growth zone in
RT Schizosaccharomyces pombe.";
RL J. Cell Sci. 118:1843-1850(2005).
RN [6]
RP REVISION OF GENE MODEL, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=21270388; DOI=10.1534/genetics.110.123497;
RA Bitton D.A., Wood V., Scutt P.J., Grallert A., Yates T., Smith D.L.,
RA Hagan I.M., Miller C.J.;
RT "Augmented annotation of the Schizosaccharomyces pombe genome reveals
RT additional genes required for growth and viability.";
RL Genetics 187:1207-1217(2011).
CC -!- FUNCTION: Required for cellular morphogenesis and polarization of the
CC cortical cytoskeleton. Required for establishment of new polarized
CC growth zones where it acts in actin organization. Involved plasma
CC membrane internalization and is essential for fluid-phase endocytosis.
CC {ECO:0000269|PubMed:15300681, ECO:0000269|PubMed:15827087}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:15827087}. Note=Localizes at cell ends during
CC interphase and to the medial ring at cell division.
CC -!- SIMILARITY: Belongs to the SLA2 family. {ECO:0000305}.
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DR EMBL; CU329670; CAB90777.2; -; Genomic_DNA.
DR EMBL; D89267; BAA13928.1; -; mRNA.
DR PIR; T43195; T43195.
DR RefSeq; NP_594069.2; NM_001019493.2.
DR AlphaFoldDB; Q9P6L5; -.
DR SMR; Q9P6L5; -.
DR BioGRID; 279759; 19.
DR STRING; 4896.SPAC688.11.1; -.
DR MaxQB; Q9P6L5; -.
DR PaxDb; Q9P6L5; -.
DR EnsemblFungi; SPAC688.11.1; SPAC688.11.1:pep; SPAC688.11.
DR GeneID; 2543336; -.
DR KEGG; spo:SPAC688.11; -.
DR PomBase; SPAC688.11; end4.
DR VEuPathDB; FungiDB:SPAC688.11; -.
DR eggNOG; KOG0980; Eukaryota.
DR HOGENOM; CLU_004601_0_0_1; -.
DR InParanoid; Q9P6L5; -.
DR OMA; IREYVYF; -.
DR Reactome; R-SPO-8856828; Clathrin-mediated endocytosis.
DR PRO; PR:Q9P6L5; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0030479; C:actin cortical patch; IDA:PomBase.
DR GO; GO:0032153; C:cell division site; IDA:PomBase.
DR GO; GO:0030136; C:clathrin-coated vesicle; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR GO; GO:0031097; C:medial cortex; IDA:PomBase.
DR GO; GO:0035841; C:new growing cell tip; IDA:PomBase.
DR GO; GO:0035840; C:old growing cell tip; IDA:PomBase.
DR GO; GO:0003779; F:actin binding; ISM:PomBase.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0035615; F:clathrin adaptor activity; ISS:PomBase.
DR GO; GO:0032051; F:clathrin light chain binding; IBA:GO_Central.
DR GO; GO:0043325; F:phosphatidylinositol-3,4-bisphosphate binding; IBA:GO_Central.
DR GO; GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; IBA:GO_Central.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; ISM:PomBase.
DR GO; GO:0030036; P:actin cytoskeleton organization; IMP:PomBase.
DR GO; GO:0007015; P:actin filament organization; IBA:GO_Central.
DR GO; GO:0048268; P:clathrin coat assembly; IBA:GO_Central.
DR GO; GO:0030866; P:cortical actin cytoskeleton organization; IMP:PomBase.
DR GO; GO:0006897; P:endocytosis; IMP:PomBase.
DR GO; GO:0030950; P:establishment or maintenance of actin cytoskeleton polarity; IMP:PomBase.
DR Gene3D; 1.25.40.90; -; 1.
DR InterPro; IPR011417; ANTH_dom.
DR InterPro; IPR013809; ENTH.
DR InterPro; IPR008942; ENTH_VHS.
DR InterPro; IPR035964; I/LWEQ_dom_sf.
DR InterPro; IPR002558; ILWEQ_dom.
DR InterPro; IPR030224; Sla2_fam.
DR PANTHER; PTHR10407; PTHR10407; 1.
DR Pfam; PF07651; ANTH; 1.
DR Pfam; PF01608; I_LWEQ; 1.
DR SMART; SM00273; ENTH; 1.
DR SMART; SM00307; ILWEQ; 1.
DR SUPFAM; SSF109885; SSF109885; 1.
DR SUPFAM; SSF48464; SSF48464; 1.
DR PROSITE; PS50942; ENTH; 1.
DR PROSITE; PS50945; I_LWEQ; 1.
PE 1: Evidence at protein level;
KW Actin-binding; Coiled coil; Cytoplasm; Cytoskeleton; Endocytosis;
KW Reference proteome.
FT CHAIN 1..1102
FT /note="Endocytosis protein end4"
FT /id="PRO_0000071944"
FT DOMAIN 9..139
FT /note="ENTH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00243"
FT DOMAIN 858..1100
FT /note="I/LWEQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00292"
FT REGION 265..334
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 338..661
FT /evidence="ECO:0000255"
SQ SEQUENCE 1102 AA; 124373 MW; 9101E230871A5DD0 CRC64;
MSSFRLQSDH MQSDASLMTS VRKATSIDET APKRKHVRSC IIFTWDHHTA RPFWTAIKVQ
PLLANEVQTF KALITIHRVL QEGHKSALVD SQSEKGWLKT CERQYDGESS PKGYSDLIRD
YVDYLLDKLS FHAQHPEFNG TFEYKEYISL RQVDDPNEGY ETVYDMMNLQ DHIDEFQKQL
FSNFKRSNKN ECRIAALVPL VQESYGIYRF LTSMLRALYS TVDAPETLEP LKHRYKSQHH
RLRQFYADCS NLRYLTSLIS VPRLPHDPPD LEGDDNIPDL PKRPASIAPQ PTGASTIAPQ
PTGTSPSPPV EMNFPDTSDI TPAYSEPEPI QDFWSDPTLD QQLAAQQAAQ QAAQQQAELA
AQQAAAQQAQ LAAQQAAEME RQRMAAQQHQ QALEAIQMAQ AEQQRIAQEQ LAQQQFQMQT
QGQLAELEQQ LLATRGQLEQ SNVLLNQYDA RVRTLENELS QAGVNLQEQI HQNDDLIESL
KNQILTWKNK YEALAKLYTQ LRQEHLDLLS KYKQIQLKAS SAQEAIDKKE KMEREMKNKN
LELADMILER DRARHELETM HRSQRDKQES TERELRLLQE KAASLERNKS SEVSNLLSRY
NTEVAHLEDA LHSKDRELAN LGVELKSTEN RYRQLLQEKE EELEIQKAAV DESLLQLSKL
QLDRNDIDQA MDTQIDELLK SQLEKLDDIV DSVLATGIQR LDTSLYELDS PMHAGNQYAT
PEFILSTIEN ASNNATDFST AFNNYFADGP NADHSEVING VNLFSTAIYE VANNAKGLSR
TTGDDQGSDR FVGLSRDLVN MAKRFLSSLF SVNTRKMDVN VKTDLVIGEN IELQRYLQQL
TQYSEKFLNK ESENTVGLLN APGENIEELV DNQLAETAQA IQQAILRLQN IAAKPKDDSL
SPSELQVHDS LLSASIAITE AIARLIKAAT ASQAEIVAQG RGSSSRGAFY KKHNRWTEGL
ISAAKAVARA TTTLIETADG VVNGTSSFEH LIVACNGVSA ATAQLVAASR VKANFASKVQ
DHLEDAAKAV TEACKALVRQ VESVALKAKE VQHEDFSSLG VHEYRRKEIE QQVQILKLEN
DLVAARRRLF DMRKTSYHVA EE
