SLAH2_ARATH
ID SLAH2_ARATH Reviewed; 519 AA.
AC Q9ASQ7; Q9SUE2;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=S-type anion channel SLAH2;
DE AltName: Full=SLAC1-homolog protein 2;
GN Name=SLAH2; OrderedLocusNames=At4g27970; ORFNames=T13J8.80;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=18305482; DOI=10.1038/nature06720;
RA Negi J., Matsuda O., Nagasawa T., Oba Y., Takahashi H., Kawai-Yamada M.,
RA Uchimiya H., Hashimoto M., Iba K.;
RT "CO2 regulator SLAC1 and its homologues are essential for anion homeostasis
RT in plant cells.";
RL Nature 452:483-486(2008).
CC -!- FUNCTION: Slow, weak voltage-dependent S-type anion efflux channel
CC involved in maintenance of anion homeostasis.
CC {ECO:0000269|PubMed:18305482}.
CC -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18305482};
CC Multi-pass membrane protein {ECO:0000269|PubMed:18305482}.
CC -!- TISSUE SPECIFICITY: Expressed in lateral root primordia and tap root
CC tips. {ECO:0000269|PubMed:18305482}.
CC -!- SIMILARITY: Belongs to the SLAC1 S-type anion channel family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB36766.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79599.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL035524; CAB36766.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161572; CAB79599.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85415.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66084.1; -; Genomic_DNA.
DR EMBL; AF367337; AAK32924.1; -; mRNA.
DR EMBL; BT002238; AAN72249.1; -; mRNA.
DR PIR; T02898; T02898.
DR RefSeq; NP_001320078.1; NM_001341891.1.
DR RefSeq; NP_567792.1; NM_118935.3.
DR AlphaFoldDB; Q9ASQ7; -.
DR SMR; Q9ASQ7; -.
DR BioGRID; 14197; 3.
DR IntAct; Q9ASQ7; 3.
DR STRING; 3702.AT4G27970.1; -.
DR iPTMnet; Q9ASQ7; -.
DR PaxDb; Q9ASQ7; -.
DR PRIDE; Q9ASQ7; -.
DR ProteomicsDB; 232511; -.
DR EnsemblPlants; AT4G27970.1; AT4G27970.1; AT4G27970.
DR EnsemblPlants; AT4G27970.6; AT4G27970.6; AT4G27970.
DR GeneID; 828910; -.
DR Gramene; AT4G27970.1; AT4G27970.1; AT4G27970.
DR Gramene; AT4G27970.6; AT4G27970.6; AT4G27970.
DR KEGG; ath:AT4G27970; -.
DR Araport; AT4G27970; -.
DR TAIR; locus:2132987; AT4G27970.
DR eggNOG; ENOG502QQKN; Eukaryota.
DR HOGENOM; CLU_017679_1_0_1; -.
DR InParanoid; Q9ASQ7; -.
DR OMA; WARIRGD; -.
DR OrthoDB; 450944at2759; -.
DR PhylomeDB; Q9ASQ7; -.
DR PRO; PR:Q9ASQ7; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9ASQ7; baseline and differential.
DR Genevisible; Q9ASQ7; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0008308; F:voltage-gated anion channel activity; IEA:InterPro.
DR GO; GO:0006873; P:cellular ion homeostasis; IEA:InterPro.
DR Gene3D; 1.50.10.150; -; 1.
DR InterPro; IPR030183; SLAC/SLAH.
DR InterPro; IPR004695; SLAC1/Mae1/Ssu1/TehA.
DR InterPro; IPR038665; Voltage-dep_anion_channel_sf.
DR PANTHER; PTHR31269; PTHR31269; 1.
DR Pfam; PF03595; SLAC1; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion transport; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..519
FT /note="S-type anion channel SLAH2"
FT /id="PRO_0000404261"
FT TOPO_DOM 1..140
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..185
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 207..220
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..247
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 248..268
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 269..281
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 303..304
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326..340
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 362
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 363..383
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 384..389
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 411..424
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 446..519
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 495..519
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..29
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 505..519
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 77
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LD83"
FT MOD_RES 85
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LD83"
SQ SEQUENCE 519 AA; 58820 MW; C178C4889CF60A7A CRC64;
MNNPRSVSPL VSPANHSDLL ENQRQSGSGD FSRLEKRIGA RKMKFHSKSM PRGAMFLDQE
ASRNFHDKRY DLFRTMSGKL ERQISNLRGK PTESSLQDHK EITESLTADR YFDALQGPEL
ETLKEKEKIV LPEDKTWPFL LRFPITSYGM CLGVSSQAIM WKTLATTEAE KFLHVTQVIN
HVLWWISLLL LLAVSITYLF KTILFFEAVR REFRHPIRVN FFFAPLISIL FLALGIPHSI
ISHLPSTLWY FLMAPILFLE MKIYGQWMSG GQRRLSKVAN PTNHLSIVGN FAGALLGASM
GLKEGPIFFF AIGLAYYLVL FVTLYQRLPT NETLPKELHP VFFLFVAAPA VASMAWTKIS
ASFDLGSRLA YFISLFLYFS LVCRINLFRG FKFSLAWWAY TFPMTAVASA TIKYSDEVTG
VATKILSVVM SGAATLTVIA VLGLTVMHAF VQRDLFPNDV VIAISAEQPK QKRWFKHLTK
ESYGNNERCP KILDPEDNQI DLESPPLVNV DSSTVQNSN
