SLAI1_MOUSE
ID SLAI1_MOUSE Reviewed; 579 AA.
AC Q68FF7; Q8R3I6;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=SLAIN motif-containing protein 1;
GN Name=Slain1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and FVB/N-3; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX PubMed=16546155; DOI=10.1016/j.ydbio.2006.01.023;
RA Hirst C.E., Ng E.S., Azzola L., Voss A.K., Thomas T., Stanley E.G.,
RA Elefanty A.G.;
RT "Transcriptional profiling of mouse and human ES cells identifies SLAIN1, a
RT novel stem cell gene.";
RL Dev. Biol. 293:90-103(2006).
RN [3]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=20563991; DOI=10.1387/ijdb.092998ch;
RA Hirst C.E., Lim S.M., Pereira L.A., Mayberry R.A., Stanley E.G.,
RA Elefanty A.G.;
RT "Expression from a betageo gene trap in the Slain1 gene locus is
RT predominantly associated with the developing nervous system.";
RL Int. J. Dev. Biol. 54:1383-1388(2010).
RN [4]
RP FUNCTION, INTERACTION WITH MAPRE1; MAPRE2; MAPRE3 AND CKAP5, AND
RP SUBCELLULAR LOCATION.
RX PubMed=21646404; DOI=10.1083/jcb.201012179;
RA van der Vaart B., Manatschal C., Grigoriev I., Olieric V., Gouveia S.M.,
RA Bjelic S., Demmers J., Vorobjev I., Hoogenraad C.C., Steinmetz M.O.,
RA Akhmanova A.;
RT "SLAIN2 links microtubule plus end-tracking proteins and controls
RT microtubule growth in interphase.";
RL J. Cell Biol. 193:1083-1099(2011).
RN [5]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-469 AND ARG-554, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- FUNCTION: Microtubule plus-end tracking protein that might be involved
CC in the regulation of cytoplasmic microtubule dynamics, microtubule
CC organization and microtubule elongation. {ECO:0000269|PubMed:21646404}.
CC -!- SUBUNIT: Interacts with MAPRE1, MAPRE2, MAPRE3 and CKAP5
CC (PubMed:21646404). Interacts with ZDHHC17 (via ANK repeats) (By
CC similarity). {ECO:0000250|UniProtKB:Q8ND83,
CC ECO:0000269|PubMed:21646404}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:21646404}. Note=Colocalizes with microtubules.
CC Detected at the plus end of growing microtubules.
CC {ECO:0000269|PubMed:21646404}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q68FF7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q68FF7-2; Sequence=VSP_030833;
CC -!- TISSUE SPECIFICITY: Expressed in embryonic stem cells
CC (PubMed:16546155). Expressed in adult bone marrow, brain, kidney, lung,
CC testis and thymus (PubMed:16546155, PubMed:20563991). Expressed in
CC colon (PubMed:20563991). Isoform 1 is highly expressed in brain
CC (PubMed:20563991). Isoform 2 is more widely expressed in bone marrow,
CC brain, colon, kidney, lung and thymus (PubMed:20563991).
CC {ECO:0000269|PubMed:16546155, ECO:0000269|PubMed:20563991}.
CC -!- DEVELOPMENTAL STAGE: During embryonic stem cell differentiation,
CC expression peaks at d2 and d3 (epiblast stage) (PubMed:16546155). In
CC postimplantation embryos widely expressed throughout 6.5-7.0 dpc,
CC followed by higher levels of expression in the headfold neurectoderm at
CC 7.5 dpc (PubMed:16546155). At 8.5 dpc, observed in the neural tube and
CC optic vesicles (PubMed:16546155). At 9.0-9.5 dpc, expressed at sites of
CC imminent neural tube closure in the midbrain, hindbrain, and tailbud
CC and in the dorsal aspects of the somites (PubMed:16546155). At 9.5 dpc,
CC expressed within the neuroepithelium surrounding the telencephalic,
CC mesencephalic and optic vesicles and in neural crest cells on either
CC side of the mesencephalic vesicles, along the neural tube continuous
CC with the tailbud, in the neural crest-derived dorsal root ganglia, in
CC the surface ectoderm of the branchial arches as well as the primitive
CC gut tube (PubMed:20563991). At 11.5 dpc, predominantly expressed within
CC the forebrain, eye and neural tube, and weaker expression within the
CC optic and oropharyngeal regions, in the epithelium of the olfactory
CC pit, optic stalk and otic vesicle, parts of the gut tube, particularly
CC within the lumen of the midgut loop and the stomach, in branchial
CC arches and in the condensing mesenchyme of the developing limb buds
CC (PubMed:20563991). At 11.5 dpc, also expressed within the
CC neuroepithelium surrounding both the fourth and telencephalic
CC ventricles (PubMed:20563991). At 13.5 dpc, expressed throughout the
CC developing nervous system with most prominent expression in forebrain,
CC midbrain and spinal cord, including the dorsal root ganglia and the
CC olfactory bulb and within the endoderm derived midgut loop in the
CC physiological umbilical hernia, as well as in the eye and in the main
CC bronchi of the lung and within the developing metanephric tubules
CC (PubMed:20563991). At 13.5 dpc, also expressed at the superficial
CC layers of the neuroepithelium and the ependymal layers surrounding the
CC fourth and lateral ventricles and in neurons of the dorsal root ganglia
CC and in dorsal roots between the cartilage primordia as well as in
CC cranial sensory ganglia (PubMed:20563991). Also expressed along the
CC apical epidermal ridge and in the forelimb and hind limb
CC (PubMed:20563991). {ECO:0000269|PubMed:16546155,
CC ECO:0000269|PubMed:20563991}.
CC -!- SIMILARITY: Belongs to the SLAIN motif-containing family.
CC {ECO:0000305}.
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DR EMBL; BC025223; AAH25223.1; -; mRNA.
DR EMBL; BC079866; AAH79866.1; -; mRNA.
DR CCDS; CCDS27316.1; -. [Q68FF7-1]
DR RefSeq; NP_932131.2; NM_198014.2. [Q68FF7-1]
DR AlphaFoldDB; Q68FF7; -.
DR SMR; Q68FF7; -.
DR BioGRID; 222856; 15.
DR IntAct; Q68FF7; 15.
DR STRING; 10090.ENSMUSP00000070592; -.
DR iPTMnet; Q68FF7; -.
DR PhosphoSitePlus; Q68FF7; -.
DR EPD; Q68FF7; -.
DR jPOST; Q68FF7; -.
DR MaxQB; Q68FF7; -.
DR PaxDb; Q68FF7; -.
DR PRIDE; Q68FF7; -.
DR ProteomicsDB; 261190; -. [Q68FF7-1]
DR ProteomicsDB; 261191; -. [Q68FF7-2]
DR Antibodypedia; 24633; 158 antibodies from 27 providers.
DR DNASU; 105439; -.
DR Ensembl; ENSMUST00000069443; ENSMUSP00000070592; ENSMUSG00000055717. [Q68FF7-1]
DR GeneID; 105439; -.
DR KEGG; mmu:105439; -.
DR UCSC; uc007uws.1; mouse. [Q68FF7-1]
DR CTD; 122060; -.
DR MGI; MGI:2145578; Slain1.
DR VEuPathDB; HostDB:ENSMUSG00000055717; -.
DR eggNOG; ENOG502QVWF; Eukaryota.
DR GeneTree; ENSGT00390000017860; -.
DR InParanoid; Q68FF7; -.
DR OMA; MSPLQWC; -.
DR OrthoDB; 658136at2759; -.
DR PhylomeDB; Q68FF7; -.
DR TreeFam; TF331616; -.
DR BioGRID-ORCS; 105439; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Slain1; mouse.
DR PRO; PR:Q68FF7; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q68FF7; protein.
DR Bgee; ENSMUSG00000055717; Expressed in animal zygote and 210 other tissues.
DR ExpressionAtlas; Q68FF7; baseline and differential.
DR Genevisible; Q68FF7; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR GO; GO:0007020; P:microtubule nucleation; IBA:GO_Central.
DR GO; GO:0031116; P:positive regulation of microtubule polymerization; IBA:GO_Central.
DR InterPro; IPR026179; Slain.
DR PANTHER; PTHR22406; PTHR22406; 2.
DR Pfam; PF15301; SLAIN; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton; Methylation;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..579
FT /note="SLAIN motif-containing protein 1"
FT /id="PRO_0000316963"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 60..95
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 135..162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 233..258
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 289..313
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 347..454
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 479..516
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 21..56
FT /evidence="ECO:0000255"
FT COMPBIAS 60..74
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 234..258
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 289..310
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 347..398
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 408..436
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 241
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8ND83"
FT MOD_RES 469
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 554
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT VAR_SEQ 1..261
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_030833"
SQ SEQUENCE 579 AA; 61307 MW; 41D009ADAA1C6439 CRC64;
MMAEQVKCAS PVAASGAGPG PVVNAELEVK KLQELVRKLE KQNEQLRSRA ASAAAAPHLL
LLQPPPPSAP PPAGACSPLA THRAPASTTS PGPGALGPAF PGTYCLPSPA PSLLCSLQPA
DAPFVYSKPA AGFFGGGGSP EPGTAGTPPG EAATPPLPPP TLLDEVEPLD LESLAAWSEE
DDYTWLYVGS SKTFTSPEKS PSPLQWCRHV LDNPTPEMEA ARRSLRFRLE QGYTSRGSPL
SPQSSIDSEL STSELEDDSI SMGYKLQDLT DVQIMARLQE ESLRQDYAST SASVSRNSSS
VSLSSGKKGT CSDQEYDRYS LEDEEEFDHL PPPQPRLPRC SPFQRGIPHS QTFSSIRDCR
RSPSTQYFPS NNFQQPQYYP PQAQTADQQP NRTNGDKLRR SMPNLARMPS TAAASSNLSS
PVTVRSSQSF DSSLHGAGSG VSRVPSCIPS PGQIQHRVHS VGHFPVPIRQ PLKATAYVSP
TVQGSSSSGS SGSSGGSGSG MPLSNGTQLY STTGIPTPNK AAASGILGRS ALPRPSLAIN
GSNLPRSKIA QPVRSFLQPP KPLSSLSTLR DGNWRDGCY
