SLAM_HAEI8
ID SLAM_HAEI8 Reviewed; 486 AA.
AC Q4QLR4;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=Surface lipoprotein assembly modifier {ECO:0000303|PubMed:28620585};
DE Short=Slam {ECO:0000303|PubMed:28620585};
DE Flags: Precursor;
GN OrderedLocusNames=NTHI1171;
OS Haemophilus influenzae (strain 86-028NP).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=281310;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=86-028NP;
RX PubMed=15968074; DOI=10.1128/jb.187.13.4627-4636.2005;
RA Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B.,
RA Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O.,
RA Munson R.S. Jr.;
RT "Genomic sequence of an otitis media isolate of nontypeable Haemophilus
RT influenzae: comparative study with H. influenzae serotype d, strain KW20.";
RL J. Bacteriol. 187:4627-4636(2005).
RN [2]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=86-028NP;
RX PubMed=28620585; DOI=10.3389/fcimb.2017.00207;
RA Hooda Y., Lai C.C.L., Moraes T.F.;
RT "Identification of a Large Family of Slam-Dependent Surface Lipoproteins in
RT Gram-Negative Bacteria.";
RL Front. Cell. Infect. Microbiol. 7:207-207(2017).
CC -!- FUNCTION: Required for correct export to the cell surface of some cell
CC outer membrane lipoproteins (tested with TpbP) upon heterologous
CC expression in E.coli and probably also in Haemophilus.
CC {ECO:0000269|PubMed:28620585}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000305|PubMed:28620585}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DOMAIN: Consists of a soluble N-terminal domain and C-terminal probable
CC beta-barrel in the outer membrane with 14 predicted beta-strands.
CC {ECO:0000250|UniProtKB:Q9K165}.
CC -!- SIMILARITY: Belongs to the Slam family. {ECO:0000305}.
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DR EMBL; CP000057; AAX88033.1; -; Genomic_DNA.
DR RefSeq; WP_011272340.1; NC_007146.2.
DR AlphaFoldDB; Q4QLR4; -.
DR EnsemblBacteria; AAX88033; AAX88033; NTHI1171.
DR KEGG; hit:NTHI1171; -.
DR HOGENOM; CLU_034927_1_0_6; -.
DR OMA; FMPARNR; -.
DR OrthoDB; 720059at2; -.
DR Proteomes; UP000002525; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR007655; Slam.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF04575; SlipAM; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
PE 3: Inferred from homology;
KW Cell outer membrane; Membrane; Signal; Transmembrane;
KW Transmembrane beta strand.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..486
FT /note="Surface lipoprotein assembly modifier"
FT /evidence="ECO:0000255"
FT /id="PRO_0000450807"
FT TRANSMEM 199..209
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..248
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..262
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..286
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..300
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..330
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 334..344
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..367
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..382
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..404
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..419
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 437..446
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..463
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TRANSMEM 476..486
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT REGION 30..197
FT /note="N-terminal domain"
FT /evidence="ECO:0000250|UniProtKB:Q9K165"
FT REGION 198..486
FT /note="C-terminal probable beta barrel"
FT /evidence="ECO:0000250|UniProtKB:Q9K165"
SQ SEQUENCE 486 AA; 56677 MW; ECA4F6F75DE58DEB CRC64;
MKNGVKQISF LSLIGLSLIG LSLTNIAWAK VARPKNDTLT NTIQSAELKT SSFSSMPKKE
IPNRHIISLS KSQLAQHPRL VLRGLILALY QNNTQAVQLL LPLYKQFPQH DNFLLTWAKA
IDAREQGDLT QSIAYYRELF ALDASLLPLR YQLAQALFFN YENESAKIQF EKLRTEVDDE
KFLGVIDQYL LTLNQRNQWI WQVGLNFLND DNLNNAPKSG TKIGSWTAWE KESGKGGRVF
FISRKKWPWA DHFFSKTMFN GNGKYYWDNK KYNEATLRIG GGLGYQTALV EVSLFPFQEK
RWYAGGSSGT NTMKQYADKL GIRLENVDWL SKTWQISTAL EYGESRYKIR KHLDGNYYFI
SSTLFYLPKS TQFWFVGMDF HRENTQALDN AYQQKTLRLG WGQDWSYGIS SRLTFSYANR
VYREKDLIGI QQKNREYATT ITLWHRNIHF MGLTPKLSWD YQKSTSNHAF YRYDKNRIYL
EIGKIF
