ID   SLAM_PASMU              Reviewed;         509 AA.
AC   Q9CKU1;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Surface lipoprotein assembly modifier {ECO:0000303|PubMed:28620585};
DE            Short=Slam {ECO:0000303|PubMed:28620585};
DE   Flags: Precursor;
GN   OrderedLocusNames=PM1515;
OS   Pasteurella multocida (strain Pm70).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Pasteurella.
OX   NCBI_TaxID=272843;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pm70;
RX   PubMed=11248100; DOI=10.1073/pnas.051634598;
RA   May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.;
RT   "Complete genomic sequence of Pasteurella multocida Pm70.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=Pm70;
RX   PubMed=28620585; DOI=10.3389/fcimb.2017.00207;
RA   Hooda Y., Lai C.C.L., Moraes T.F.;
RT   "Identification of a Large Family of Slam-Dependent Surface Lipoproteins in
RT   Gram-Negative Bacteria.";
RL   Front. Cell. Infect. Microbiol. 7:207-207(2017).
CC   -!- FUNCTION: Required for correct export to the cell surface of some cell
CC       outer membrane lipoproteins (tested with PM1514) upon heterologous
CC       expression in E.coli and probably also in Pasteurella.
CC       {ECO:0000269|PubMed:28620585}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane
CC       {ECO:0000305|PubMed:28620585}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- DOMAIN: Consists of a soluble N-terminal domain and C-terminal probable
CC       beta-barrel in the outer membrane with 14 predicted beta-strands.
CC       {ECO:0000250|UniProtKB:Q9K165}.
CC   -!- SIMILARITY: Belongs to the Slam family. {ECO:0000305}.
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DR   EMBL; AE004439; AAK03599.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9CKU1; -.
DR   STRING; 747.DR93_364; -.
DR   EnsemblBacteria; AAK03599; AAK03599; PM1515.
DR   KEGG; pmu:PM1515; -.
DR   HOGENOM; CLU_034927_1_0_6; -.
DR   OMA; FMPARNR; -.
DR   Proteomes; UP000000809; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR007655; Slam.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   Pfam; PF04575; SlipAM; 1.
DR   SUPFAM; SSF48452; SSF48452; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Membrane; Reference proteome; Signal; TPR repeat;
KW   Transmembrane; Transmembrane beta strand.
FT   SIGNAL          1..32
FT                   /evidence="ECO:0000255"
FT   CHAIN           33..509
FT                   /note="Surface lipoprotein assembly modifier"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000450809"
FT   TRANSMEM        206..216
FT                   /note="Beta stranded"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        245..256
FT                   /note="Beta stranded"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        261..270
FT                   /note="Beta stranded"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..294
FT                   /note="Beta stranded"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        298..308
FT                   /note="Beta stranded"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        331..341
FT                   /note="Beta stranded"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        345..355
FT                   /note="Beta stranded"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        371..381
FT                   /note="Beta stranded"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        386..395
FT                   /note="Beta stranded"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        408..417
FT                   /note="Beta stranded"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        423..432
FT                   /note="Beta stranded"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        461..470
FT                   /note="Beta stranded"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        476..485
FT                   /note="Beta stranded"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        499..509
FT                   /note="Beta stranded"
FT                   /evidence="ECO:0000255"
FT   REPEAT          120..153
FT                   /note="TPR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT   REGION          33..204
FT                   /note="N-terminal domain"
FT                   /evidence="ECO:0000250|UniProtKB:Q9K165"
FT   REGION          43..72
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          205..509
FT                   /note="C-terminal probable beta barrel"
FT                   /evidence="ECO:0000250|UniProtKB:Q9K165"
SQ   SEQUENCE   509 AA;  58706 MW;  76E4A920C063CE09 CRC64;
     MNLMINLKPL TFLPFFGRLV FLSGVIYNTA WANTVIPVDN SRPDETFSQT SPKQHLFSQK
     PKPTEPTSSA SSQQISLTIE QLASRPDLVL RALIPALKNN DMRGVEILLP IYAKQSPDPF
     LLKWAQAVVA RKQGKLNESV RLYRQIIAEK PNLQPARLQL AIALTQNREN EAAKAQFNQL
     RSENLPAPLL TLIDSYIDTI NQRDSWNVYG GVNYLHENNV NNAPPKGTKA EGFTPSSQPE
     KAHGLSYFIN LSKNWSLAHG FFTEFSADIN GKYYWDNHKY DEFSTRLNLG GGYRNAKTEV
     KLMPFVEQFW YVGGENATKD ARTLHRYSKS SGINLDVDYW LTPNWKISTV LEYTEQRYIQ
     PQRQNSNGNS YSISNTLIYM PNSQQFWFVG LDYYQKNTRL KAYAFERQGI RLGWGQEWPK
     GISTRLQTSY ATRTYRAPSA EKNMIFAPSF FKVVQKNHEY GVNFTIWHRS LHWLGITPKI
     TWAYQKTTSN NPFSEYDRNR IYLTFSKTF