SLAP1_BACAN
ID SLAP1_BACAN Reviewed; 814 AA.
AC P49051; Q6I2R3; Q6KWJ4;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 25-MAY-2022, entry version 136.
DE RecName: Full=S-layer protein sap;
DE AltName: Full=Surface array protein;
DE AltName: Full=Surface layer protein;
DE Flags: Precursor;
GN Name=sap; OrderedLocusNames=BA_0885, GBAA_0885, BAS0841;
OS Bacillus anthracis.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1392;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 30-40; 209-218;
RP 281-291 AND 571-580.
RC STRAIN=Sterne;
RX PubMed=7836294; DOI=10.1128/jb.177.3.614-620.1995;
RA Etienne-Toumelin I., Sirard J., Duflot E., Mock M., Fouet A.;
RT "Characterization of the Bacillus anthracis S-layer: cloning and sequencing
RT of the structural gene.";
RL J. Bacteriol. 177:614-620(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames / isolate Porton;
RX PubMed=12721629; DOI=10.1038/nature01586;
RA Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C.,
RA Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y.,
RA Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M.,
RA Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E.,
RA White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT "The genome sequence of Bacillus anthracis Ames and comparison to closely
RT related bacteria.";
RL Nature 423:81-86(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames ancestor;
RX PubMed=18952800; DOI=10.1128/jb.01347-08;
RA Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL J. Bacteriol. 191:445-446(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sterne;
RA Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA Richardson P., Rubin E., Tice H.;
RT "Complete genome sequence of Bacillus anthracis Sterne.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The S-layer is a paracrystalline mono-layered assembly of
CC proteins which coat the surface of bacteria.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall, S-layer.
CC -!- PTM: Probably glycosylated.
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DR EMBL; Z36946; CAA85408.1; -; Genomic_DNA.
DR EMBL; AE016879; AAP24883.1; -; Genomic_DNA.
DR EMBL; AE017334; AAT29996.1; -; Genomic_DNA.
DR EMBL; AE017225; AAT53168.1; -; Genomic_DNA.
DR PIR; I40048; I40048.
DR RefSeq; NP_843397.1; NC_003997.3.
DR RefSeq; WP_001140751.1; NZ_WXXJ01000017.1.
DR RefSeq; YP_027117.1; NC_005945.1.
DR PDB; 3PYW; X-ray; 1.80 A; A=31-210.
DR PDB; 6BT4; X-ray; 2.31 A; A=31-210.
DR PDB; 6HHU; X-ray; 2.70 A; A=216-814.
DR PDB; 6QX4; X-ray; 3.27 A; A/B=216-814.
DR PDBsum; 3PYW; -.
DR PDBsum; 6BT4; -.
DR PDBsum; 6HHU; -.
DR PDBsum; 6QX4; -.
DR AlphaFoldDB; P49051; -.
DR SMR; P49051; -.
DR STRING; 261594.GBAA_0885; -.
DR ABCD; P49051; 12 sequenced antibodies.
DR EnsemblBacteria; AAP24883; AAP24883; BA_0885.
DR EnsemblBacteria; AAT29996; AAT29996; GBAA_0885.
DR GeneID; 45020949; -.
DR KEGG; ban:BA_0885; -.
DR KEGG; bar:GBAA_0885; -.
DR KEGG; bat:BAS0841; -.
DR PATRIC; fig|198094.11.peg.882; -.
DR eggNOG; COG0860; Bacteria.
DR HOGENOM; CLU_345045_0_0_9; -.
DR OMA; APQYKDA; -.
DR Proteomes; UP000000427; Chromosome.
DR Proteomes; UP000000594; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0030115; C:S-layer; IEA:UniProtKB-SubCell.
DR Gene3D; 2.60.40.1220; -; 1.
DR InterPro; IPR003343; Big_2.
DR InterPro; IPR014755; Cu-Rt/internalin_Ig-like.
DR InterPro; IPR008964; Invasin/intimin_cell_adhesion.
DR InterPro; IPR032812; SbsA_Ig.
DR InterPro; IPR001119; SLH_dom.
DR Pfam; PF02368; Big_2; 1.
DR Pfam; PF13205; Big_5; 1.
DR Pfam; PF00395; SLH; 2.
DR SMART; SM00635; BID_2; 1.
DR SUPFAM; SSF49373; SSF49373; 1.
DR PROSITE; PS51272; SLH; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Cell wall; Direct protein sequencing; Glycoprotein;
KW Reference proteome; Repeat; S-layer; Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000269|PubMed:7836294"
FT CHAIN 30..814
FT /note="S-layer protein sap"
FT /id="PRO_0000032627"
FT DOMAIN 30..93
FT /note="SLH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT DOMAIN 94..150
FT /note="SLH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT DOMAIN 152..214
FT /note="SLH 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT DOMAIN 403..479
FT /note="BIG2"
FT /evidence="ECO:0000255"
FT HELIX 44..52
FT /evidence="ECO:0007829|PDB:3PYW"
FT HELIX 72..82
FT /evidence="ECO:0007829|PDB:3PYW"
FT HELIX 96..98
FT /evidence="ECO:0007829|PDB:3PYW"
FT HELIX 104..112
FT /evidence="ECO:0007829|PDB:3PYW"
FT HELIX 131..142
FT /evidence="ECO:0007829|PDB:3PYW"
FT HELIX 144..147
FT /evidence="ECO:0007829|PDB:3PYW"
FT STRAND 148..150
FT /evidence="ECO:0007829|PDB:3PYW"
FT HELIX 157..160
FT /evidence="ECO:0007829|PDB:3PYW"
FT STRAND 163..165
FT /evidence="ECO:0007829|PDB:3PYW"
FT HELIX 166..174
FT /evidence="ECO:0007829|PDB:3PYW"
FT HELIX 193..207
FT /evidence="ECO:0007829|PDB:3PYW"
FT STRAND 217..221
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 224..228
FT /evidence="ECO:0007829|PDB:6HHU"
FT HELIX 238..240
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 241..245
FT /evidence="ECO:0007829|PDB:6HHU"
FT TURN 246..248
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 254..259
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 261..272
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 278..284
FT /evidence="ECO:0007829|PDB:6HHU"
FT TURN 285..287
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 288..294
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 301..304
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 307..310
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 313..316
FT /evidence="ECO:0007829|PDB:6QX4"
FT STRAND 319..322
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 335..338
FT /evidence="ECO:0007829|PDB:6HHU"
FT HELIX 342..344
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 349..352
FT /evidence="ECO:0007829|PDB:6QX4"
FT STRAND 358..367
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 370..374
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 378..383
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 387..397
FT /evidence="ECO:0007829|PDB:6HHU"
FT TURN 398..400
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 413..415
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 421..428
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 431..434
FT /evidence="ECO:0007829|PDB:6QX4"
FT STRAND 437..443
FT /evidence="ECO:0007829|PDB:6HHU"
FT TURN 445..447
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 448..450
FT /evidence="ECO:0007829|PDB:6HHU"
FT TURN 452..454
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 456..472
FT /evidence="ECO:0007829|PDB:6HHU"
FT TURN 474..476
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 481..489
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 494..497
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 502..507
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 514..517
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 520..522
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 532..537
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 539..541
FT /evidence="ECO:0007829|PDB:6QX4"
FT STRAND 549..554
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 557..561
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 567..578
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 581..592
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 599..603
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 607..611
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 620..623
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 625..629
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 631..633
FT /evidence="ECO:0007829|PDB:6QX4"
FT STRAND 643..646
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 662..666
FT /evidence="ECO:0007829|PDB:6HHU"
FT TURN 667..669
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 670..673
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 682..688
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 693..702
FT /evidence="ECO:0007829|PDB:6HHU"
FT HELIX 706..709
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 713..716
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 718..723
FT /evidence="ECO:0007829|PDB:6HHU"
FT HELIX 729..732
FT /evidence="ECO:0007829|PDB:6HHU"
FT HELIX 733..736
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 737..739
FT /evidence="ECO:0007829|PDB:6HHU"
FT HELIX 744..747
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 750..759
FT /evidence="ECO:0007829|PDB:6HHU"
FT TURN 761..763
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 768..770
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 775..787
FT /evidence="ECO:0007829|PDB:6HHU"
FT STRAND 790..806
FT /evidence="ECO:0007829|PDB:6HHU"
SQ SEQUENCE 814 AA; 86621 MW; C1638D26A1C6B101 CRC64;
MAKTNSYKKV IAGTMTAAMV AGVVSPVAAA GKTFPDVPAD HWGIDSINYL VEKGAVKGND
KGMFEPGKEL TRAEAATMMA QILNLPIDKD AKPSFADSQG QWYTPFIAAV EKAGVIKGTG
NGFEPNGKID RVSMASLLVE AYKLDTKVNG TPATKFKDLE TLNWGKEKAN ILVELGISVG
TGDQWEPKKT VTKAEAAQFI AKTDKQFGTE AAKVESAKAV TTQKVEVKFS KAVEKLTKED
IKVTNKANND KVLVKEVTLS EDKKSATVEL YSNLAAKQTY TVDVNKVGKT EVAVGSLEAK
TIEMADQTVV ADEPTALQFT VKDENGTEVV SPEGIEFVTP AAEKINAKGE ITLAKGTSTT
VKAVYKKDGK VVAESKEVKV SAEGAAVASI SNWTVAEQNK ADFTSKDFKQ NNKVYEGDNA
YVQVELKDQF NAVTTGKVEY ESLNTEVAVV DKATGKVTVL SAGKAPVKVT VKDSKGKELV
SKTVEIEAFA QKAMKEIKLE KTNVALSTKD VTDLKVKAPV LDQYGKEFTA PVTVKVLDKD
GKELKEQKLE AKYVNKELVL NAAGQEAGNY TVVLTAKSGE KEAKATLALE LKAPGAFSKF
EVRGLEKELD KYVTEENQKN AMTVSVLPVD ANGLVLKGAE AAELKVTTTN KEGKEVDATD
AQVTVQNNSV ITVGQGAKAG ETYKVTVVLD GKLITTHSFK VVDTAPTAKG LAVEFTSTSL
KEVAPNADLK AALLNILSVD GVPATTAKAT VSNVEFVSAD TNVVAENGTV GAKGATSIYV
KNLTVVKDGK EQKVEFDKAV QVAVSIKEAK PATK
