ID   SLAP2_BACAN             Reviewed;         862 AA.
AC   P94217; Q6I2R2; Q6KWJ3;
DT   24-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 122.
DE   RecName: Full=S-layer protein EA1;
DE   Flags: Precursor;
GN   Name=eag; OrderedLocusNames=BA_0887, GBAA_0887, BAS0842;
OS   Bacillus anthracis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Sterne / 9131;
RX   PubMed=9106206; DOI=10.1046/j.1365-2958.1997.2941659.x;
RA   Mesnage S., Tosi-Couture E., Mock M., Gounon P., Fouet A.;
RT   "Molecular characterization of the Bacillus anthracis main S-layer
RT   component: evidence that it is the major cell-associated antigen.";
RL   Mol. Microbiol. 23:1147-1155(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames / isolate Porton;
RX   PubMed=12721629; DOI=10.1038/nature01586;
RA   Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA   Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA   Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA   Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA   DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C.,
RA   Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y.,
RA   Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M.,
RA   Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E.,
RA   White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA   Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT   "The genome sequence of Bacillus anthracis Ames and comparison to closely
RT   related bacteria.";
RL   Nature 423:81-86(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames ancestor;
RX   PubMed=18952800; DOI=10.1128/jb.01347-08;
RA   Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA   Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT   "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL   J. Bacteriol. 191:445-446(2009).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sterne;
RA   Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA   Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA   Richardson P., Rubin E., Tice H.;
RT   "Complete genome sequence of Bacillus anthracis Sterne.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The S-layer is a paracrystalline mono-layered assembly of
CC       proteins which coat the surface of bacteria.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall, S-layer.
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DR   EMBL; X99724; CAA68063.1; -; Genomic_DNA.
DR   EMBL; AE016879; AAP24884.1; -; Genomic_DNA.
DR   EMBL; AE017334; AAT29997.1; -; Genomic_DNA.
DR   EMBL; AE017225; AAT53169.1; -; Genomic_DNA.
DR   RefSeq; NP_843398.1; NC_003997.3.
DR   RefSeq; WP_001140715.1; NZ_WXXJ01000017.1.
DR   RefSeq; YP_027118.1; NC_005945.1.
DR   AlphaFoldDB; P94217; -.
DR   SMR; P94217; -.
DR   IntAct; P94217; 1.
DR   STRING; 261594.GBAA_0887; -.
DR   EnsemblBacteria; AAP24884; AAP24884; BA_0887.
DR   EnsemblBacteria; AAT29997; AAT29997; GBAA_0887.
DR   GeneID; 45020950; -.
DR   KEGG; ban:BA_0887; -.
DR   KEGG; bar:GBAA_0887; -.
DR   KEGG; bat:BAS0842; -.
DR   PATRIC; fig|198094.11.peg.883; -.
DR   eggNOG; COG4196; Bacteria.
DR   HOGENOM; CLU_330823_0_0_9; -.
DR   OMA; KAQIHNV; -.
DR   Proteomes; UP000000427; Chromosome.
DR   Proteomes; UP000000594; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0030115; C:S-layer; IDA:TIGR.
DR   GO; GO:0005198; F:structural molecule activity; IDA:TIGR.
DR   GO; GO:0045229; P:external encapsulating structure organization; IDA:TIGR.
DR   InterPro; IPR001119; SLH_dom.
DR   Pfam; PF00395; SLH; 2.
DR   PROSITE; PS51272; SLH; 3.
PE   3: Inferred from homology;
KW   Cell wall; Reference proteome; Repeat; S-layer; Secreted; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..862
FT                   /note="S-layer protein EA1"
FT                   /id="PRO_0000032628"
FT   DOMAIN          30..93
FT                   /note="SLH 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT   DOMAIN          94..151
FT                   /note="SLH 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT   DOMAIN          152..214
FT                   /note="SLH 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
SQ   SEQUENCE   862 AA;  91362 MW;  CB16B202F62CCCA0 CRC64;
     MAKTNSYKKV IAGTMTAAMV AGIVSPVAAA GKSFPDVPAG HWAEGSINYL VDKGAITGKP
     DGTYGPTESI DRASAAVIFT KILNLPVDEN AQPSFKDAKN IWSSKYIAAV EKAGVVKGDG
     KENFYPEGKI DRASFASMLV SAYNLKDKVN GELVTTFEDL LDHWGEEKAN ILINLGISVG
     TGGKWEPNKS VSRAEAAQFI ALTDKKYGKK DNAQAYVTDV KVSEPTKLTL TGTGLDKLSA
     DDVTLEGDKA VAIEASTDGT SAVVTLGGKV APNKDLTVKV KNQSFVTKFV YEVKKLAVEK
     LTFDDDRAGQ AIAFKLNDEK GNADVEYLNL ANHDVKFVAN NLDGSPANIF EGGEATSTTG
     KLAVGIKQGD YKVEVQVTKR GGLTVSNTGI ITVKNLDTPA SAIKNVVFAL DADNDGVVNY
     GSKLSGKDFA LNSQNLVVGE KASLNKLVAT IAGEDKVVDP GSISIKSSNH GIISVVNNYI
     TAEAAGEATL TIKVGDVTKD VKFKVTTDSR KLVSVKANPD KLQVVQNKTL PVTFVTTDQY
     GDPFGANTAA IKEVLPKTGV VAEGGLDVVT TDSGSIGTKT IGVTGNDVGE GTVHFQNGNG
     ATLGSLYVNV TEGNVAFKNF ELVSKVGQYG QSPDTKLDLN VSTTVEYQLS KYTSDRVYSD
     PENLEGYEVE SKNLAVADAK IVGNKVVVTG KTPGKVDIHL TKNGATAGKA TVEIVQETIA
     IKSVNFKPVQ TENFVEKKIN IGTVLELEKS NLDDIVKGIN LTKETQHKVR VVKSGAEQGK
     LYLDRNGDAV FNAGDVKLGD VTVSQTSDSA LPNFKADLYD TLTTKYTDKG TLVFKVLKDK
     DVITSEIGSQ AVHVNVLNNP NL