SLAPM_BREBN
ID SLAPM_BREBN Reviewed; 1053 AA.
AC P06546; C0Z744; O31327; Q44953;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 4.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Middle cell wall protein;
DE Short=MWP;
DE Flags: Precursor;
GN OrderedLocusNames=BBR47_54160;
OS Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus.
OX NCBI_TaxID=358681;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2828336; DOI=10.1128/jb.170.2.935-945.1988;
RA Tsuboi A., Uchihi R., Adachi T., Sasaki T., Hayakawa S., Yamagata H.,
RA Tsukagoshi N., Udaka S.;
RT "Characterization of the genes for the hexagonally arranged surface layer
RT proteins in protein-producing Bacillus brevis 47: complete nucleotide
RT sequence of the middle wall protein gene.";
RL J. Bacteriol. 170:935-945(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=47 / JCM 6285 / NBRC 100599;
RA Hosoyama A., Yamada R., Hongo Y., Terui Y., Ankai A., Masuyama W.,
RA Sekiguchi M., Takeda T., Asano K., Ohji S., Ichikawa N., Narita S.,
RA Aoki N., Miura H., Matsushita S., Sekigawa T., Yamagata H., Yoshikawa H.,
RA Udaka S., Tanikawa S., Fujita N.;
RT "Brevibacillus brevis strain 47, complete genome.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-200.
RX PubMed=3029027; DOI=10.1128/jb.169.3.1239-1245.1987;
RA Yamagata H., Adachi T., Tsuboi A., Takao M., Sasaki T., Tsukagoshi N.,
RA Udaka S.;
RT "Cloning and characterization of the 5' region of the cell wall protein
RT gene operon in Bacillus brevis 47.";
RL J. Bacteriol. 169:1239-1245(1987).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 676-1053.
RX PubMed=2428810; DOI=10.1128/jb.168.1.365-373.1986;
RA Tsuboi A., Uchihi R., Tabata R., Takahashi Y., Hashiba H., Sasaki T.,
RA Yamagata H., Tsukagoshi N., Udaka S.;
RT "Characterization of the genes coding for two major cell wall proteins from
RT protein-producing Bacillus brevis 47: complete nucleotide sequence of the
RT outer wall protein gene.";
RL J. Bacteriol. 168:365-373(1986).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
RX PubMed=2914862; DOI=10.1128/jb.171.2.1010-1016.1989;
RA Adachi T., Yamagata H., Tsukagoshi N., Udaka S.;
RT "Multiple and tandemly arranged promoters of the cell wall protein gene
RT operon in Bacillus brevis 47.";
RL J. Bacteriol. 171:1010-1016(1989).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
RX PubMed=2512285; DOI=10.1128/jb.171.12.6747-6752.1989;
RA Tsuboi A., Uchihi R., Engelhardt H., Hattori H., Shimizu S., Tsukagoshi N.,
RA Udaka S.;
RT "In vitro reconstitution of a hexagonal array with a surface layer protein
RT synthesized by Bacillus subtilis harboring the surface layer protein gene
RT from Bacillus brevis 47.";
RL J. Bacteriol. 171:6747-6752(1989).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-41.
RX PubMed=2294096; DOI=10.1128/jb.172.1.511-513.1990;
RA Adachi T., Yamagata H., Tsukagoshi N., Udaka S.;
RT "Use of both translation initiation sites of the middle wall protein gene
RT in Bacillus brevis 47.";
RL J. Bacteriol. 172:511-513(1990).
CC -!- FUNCTION: The middle wall protein binds to peptidoglycan and to the
CC outer cell wall protein.
CC -!- SUBUNIT: The middle cell wall layer is composed of subunits of the
CC middle cell wall protein. These proteins form a hexagonal array with a
CC lattice constant of 14.5 nM in the middle cell wall layers.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall, S-layer. Note=This bacterium
CC is covered by a S-layer with hexagonal symmetry.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA22617.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M19115; AAA22760.1; -; Genomic_DNA.
DR EMBL; AP008955; BAH46393.1; -; Genomic_DNA.
DR EMBL; M15364; AAA87321.1; -; Genomic_DNA.
DR EMBL; M14238; AAA22372.1; -; Genomic_DNA.
DR EMBL; M31828; AAA22619.1; -; Genomic_DNA.
DR EMBL; M32356; AAA22617.1; ALT_INIT; Genomic_DNA.
DR EMBL; M32356; AAA22618.1; -; Genomic_DNA.
DR PIR; A28555; A28555.
DR RefSeq; WP_015893589.1; NC_012491.1.
DR AlphaFoldDB; P06546; -.
DR SMR; P06546; -.
DR STRING; 358681.BBR47_54160; -.
DR EnsemblBacteria; BAH46393; BAH46393; BBR47_54160.
DR KEGG; bbe:BBR47_54160; -.
DR eggNOG; COG2373; Bacteria.
DR HOGENOM; CLU_286159_0_0_9; -.
DR OrthoDB; 149270at2; -.
DR Proteomes; UP000001877; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0030115; C:S-layer; IEA:UniProtKB-SubCell.
DR InterPro; IPR001119; SLH_dom.
DR Pfam; PF00395; SLH; 2.
DR PROSITE; PS51272; SLH; 2.
PE 4: Predicted;
KW Cell wall; Reference proteome; Repeat; S-layer; Secreted; Signal.
FT SIGNAL 1..23
FT CHAIN 24..1053
FT /note="Middle cell wall protein"
FT /id="PRO_0000032642"
FT DOMAIN 26..89
FT /note="SLH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT DOMAIN 90..153
FT /note="SLH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT DOMAIN 154..203
FT /note="SLH 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT CONFLICT 864
FT /note="S -> R (in Ref. 1; AAA22760 and 4; AAA22372)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1053 AA; 117078 MW; 2F07E7A88C2DEF4F CRC64;
MKKVVNSVLA SALALTVAPM AFAAEEAATT TAPKMDADME KTVKRLEALG LVAGYGNGEY
GVDKTITRAE FATLVVRARG LEQGAKLAQF SNTYTDVKST DWFAGFVNVA SGEEIVKGFP
DKSFKPQNQV TYAEAVTMIV RALGYEPSVK GVWPNSMISK ASELNIARSI TTPNNAATRG
DIFKMLDNAL RVDLMEQVEF GTDIRHEITK ETLLTKYLKV TVRDMEWAQE AGNDSEDLPL
VTNVPAIGLG KIKANEVTLN GKDAGIGNTT YKVADGINAN DFDGQHVQVW IKDDKEDVIV
WMEGSTDQEV IMDRVGEFTL KGKTFEDPKD LSNSDLADLK LELDASEKSY RFNKNTKVTY
NFTRFNDPVD GLKEIIKDNA DGGFTFGAKV VLDNNNEIAY IHVIDDQSMN KEDEGVKYGS
EVISKIDTDK KKITNRDNDK FNDLDGKEEG KDFLVFLNGK PAKFSDLKEG MVYSVYYADG
DEDKLLVFAT DTVVEGKVDK VVSRNNNDYR LTIGDKTYRV YEGATFSDDG NKDVQDIDKD
HWDLVDSLDD ETVKLYLDAS GRVRHIETKD AIDDRKQKAI VTRSATFNTS KDTWDFRVLT
QKGKEITVSL EAKNIYDFDG KNFSRDNKNQ DDLEDILVPS KDKDTLLLEV TLDADGKPGK
VEFLKPVKVE QESGKAWDDL ADEDDDMVGD YEVTDKTAVF NMTGKLEESS KRKELKNAKT
AKFKDVADEN DLSVIYTVND KDEVEAIFVV EGDGLTGDAH YGQVIDFGRK GGKDTIRVWE
KDGDKVVEKE YKLDGDQDDL KDEDIRRNDF IAFTVDSNDE VVVDDVVEVV NKNAKGMLAE
VTDEKGMKDA NIDKMVVGLV SDVSKDTITY KDADDNKKKA SIKSATVYFD LYDDFGEADG
VNEGDYVVMI DSGDISGTKY DYVLIVSDAK TVRKDKLEDD AEAFLKQEPS EKPDPDTKWD
ALPSKVEGKF TSAGPVKLYR ATVELNSKVK AEDVDAIEFY FNGKKVEPSL LNFKDGVITI
GYNTEDKVTS SKIKVTNKNG KDSDEATVTF VEA
