ID   SLAPO_BREBN             Reviewed;        1004 AA.
AC   P09333; C0Z743;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Outer cell wall protein;
DE            Short=OWP;
DE   Flags: Precursor;
GN   OrderedLocusNames=BBR47_54150;
OS   Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus.
OX   NCBI_TaxID=358681;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF N-TERMINUS.
RX   PubMed=2428810; DOI=10.1128/jb.168.1.365-373.1986;
RA   Tsuboi A., Uchihi R., Tabata R., Takahashi Y., Hashiba H., Sasaki T.,
RA   Yamagata H., Tsukagoshi N., Udaka S.;
RT   "Characterization of the genes coding for two major cell wall proteins from
RT   protein-producing Bacillus brevis 47: complete nucleotide sequence of the
RT   outer wall protein gene.";
RL   J. Bacteriol. 168:365-373(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=47 / JCM 6285 / NBRC 100599;
RA   Hosoyama A., Yamada R., Hongo Y., Terui Y., Ankai A., Masuyama W.,
RA   Sekiguchi M., Takeda T., Asano K., Ohji S., Ichikawa N., Narita S.,
RA   Aoki N., Miura H., Matsushita S., Sekigawa T., Yamagata H., Yoshikawa H.,
RA   Udaka S., Tanikawa S., Fujita N.;
RT   "Brevibacillus brevis strain 47, complete genome.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-85.
RX   PubMed=2828336; DOI=10.1128/jb.170.2.935-945.1988;
RA   Tsuboi A., Uchihi R., Adachi T., Sasaki T., Hayakawa S., Yamagata H.,
RA   Tsukagoshi N., Udaka S.;
RT   "Characterization of the genes for the hexagonally arranged surface layer
RT   proteins in protein-producing Bacillus brevis 47: complete nucleotide
RT   sequence of the middle wall protein gene.";
RL   J. Bacteriol. 170:935-945(1988).
CC   -!- FUNCTION: The outer wall protein binds to the middle cell wall protein.
CC   -!- SUBUNIT: The outer cell wall layer is composed of subunits of the outer
CC       cell wall protein. These proteins form a hexagonal array with a lattice
CC       constant of 14.5 nm in the outer cell wall layers.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall, S-layer. Note=This bacterium
CC       is covered by a S-layer with hexagonal symmetry.
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DR   EMBL; M14238; AAA22373.1; -; Genomic_DNA.
DR   EMBL; AP008955; BAH46392.1; -; Genomic_DNA.
DR   EMBL; M19115; AAA22761.1; -; Genomic_DNA.
DR   PIR; B25039; B25039.
DR   RefSeq; WP_015893588.1; NC_012491.1.
DR   AlphaFoldDB; P09333; -.
DR   SMR; P09333; -.
DR   STRING; 358681.BBR47_54150; -.
DR   EnsemblBacteria; BAH46392; BAH46392; BBR47_54150.
DR   KEGG; bbe:BBR47_54150; -.
DR   eggNOG; COG0544; Bacteria.
DR   HOGENOM; CLU_298927_0_0_9; -.
DR   OMA; IAFTEPM; -.
DR   OrthoDB; 610682at2; -.
DR   Proteomes; UP000001877; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0030115; C:S-layer; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.60.40.10; -; 1.
DR   Gene3D; 2.60.40.1220; -; 5.
DR   InterPro; IPR014755; Cu-Rt/internalin_Ig-like.
DR   InterPro; IPR013783; Ig-like_fold.
PE   1: Evidence at protein level;
KW   Cell wall; Direct protein sequencing; Reference proteome; S-layer;
KW   Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000269|PubMed:2428810"
FT   CHAIN           25..1004
FT                   /note="Outer cell wall protein"
FT                   /id="PRO_0000032643"
SQ   SEQUENCE   1004 AA;  106137 MW;  3534F65EBD0EDB9E CRC64;
     MNKKVVLSVL STTLVASVAA SAFAAPKDGI YIGGNIKKYY STDVLFEMTP QAKATYASEL
     NAMASDFNNV VFVDYKGKGA SIEELFTKGS KVALGEPLKK EDFADLYKVV NKDGSSTATE
     DARAKVDPTP TGDLNVESVS ANNLKEVVVT FDKAVDADTA GDKAYYTFTA NKLAVDKVTV
     SGKTVVLTLA AKAENQASYE LNVDGIKGLV KTTKEVKFFD NTTPTVAAVA AIGPKQVKVT
     FSEPLSAKPS FSVNNGAIAV VADNFVEGTK EVILTLGAQP TASTNTVTVE GGADYASYKV
     EKVTKDFTVV ADTTPPTVSV KKASAKQVVL EFSEDVQNVQ DKNVVFYHTT KGHEGYKGTI
     LGVDGKEVTI SFVNPLPEGQ FKIFVDYVVD NGTQISDLWG NKLPEQVITG TFAADTTPPT
     VTKVEAKTNT EIHVTFSETV NGADNKANFT LKGVTGNVIP LTKAEVVDAA KNIYKVVTTE
     PLNGGSYYLT VKGIEDASKN KLVEYTATVA VADTVPPNVK DLDPATPGTD AQLISPTKVK
     IAFTEPMDKA SIENKNNYMF NGFNLDSKVT LTATDSNTAV VVDFTNVVGF NGFKNGDAIS
     VGRVLDTAGN PKTEMQTKVN LPNSVSAPLF DKAEVTGKNT VKLYFKELII NAKADDFAVD
     NGEGYKAVNS ISNDVVENKS VITLTTGNDL PTTAAGVKVK TVGEVDAKNQ YGVAVALTDV
     PADDKIGPNW LKAETVDTNN NGKIDQFKLT FSEALYVASV QDSDFRIEGY TIAGVETKGE
     VVTIKVTELD IDDSDATPTV AVIGSVEDLK RNASGPFEPQ KAIDGVSAPD KEAPVVTGVE
     AGKTYNTAVT PDSADKDIKT VVLKKDGKEL AGYALKTPIS ENGSYELVVT DNAGNTTTVK
     FKVDIPAEDK KAPEIKTVTD DKVAVADAPK WEAPKATATD DVDGDISDKI AVTYSSEDAG
     SKVTDLASAQ THLGTAGNTV KVTYNVTDKA GNPATAVSAT FTAI