ID   SLAP_AERSA              Reviewed;         502 AA.
AC   P35823;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=S-layer protein {ECO:0000305};
DE   AltName: Full=A-layer protein {ECO:0000305};
DE   AltName: Full=Cell surface virulent A protein {ECO:0000303|PubMed:6871174};
DE            Short=A-protein {ECO:0000303|PubMed:6871174};
DE   AltName: Full=Tetragonal surface virulence array protein {ECO:0000303|PubMed:1869553};
DE   Flags: Precursor;
GN   Name=vapA {ECO:0000303|PubMed:1869553};
OS   Aeromonas salmonicida.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=645;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, AND DOMAIN.
RC   STRAIN=A450;
RX   PubMed=1869553; DOI=10.1016/s0021-9258(18)98611-4;
RA   Chu S., Cavaignac S., Feutrier J., Phipps B.M., Kostrzynska M., Kay W.W.,
RA   Trust T.J.;
RT   "Structure of the tetragonal surface virulence array protein and gene of
RT   Aeromonas salmonicida.";
RL   J. Biol. Chem. 266:15258-15265(1991).
RN   [2]
RP   PROTEIN SEQUENCE OF 22-48.
RC   STRAIN=A400, A450, and A461;
RX   PubMed=6525563; DOI=10.1139/o84-137;
RA   Kay W.W., Phipps B.M., Ishiguro E.E., Olafson R.W., Trust T.J.;
RT   "Surface layer virulence A-proteins from Aeromonas salmonicida strains.";
RL   Can. J. Biochem. Cell Biol. 62:1064-1071(1984).
RN   [3]
RP   PROTEIN SEQUENCE OF 22-48, SUBUNIT, AND SUBCELLULAR LOCATION.
RC   STRAIN=A450;
RX   PubMed=6871174; DOI=10.1021/bi00281a023;
RA   Phipps B.M., Trust T.J., Ishiguro E.E., Kay W.W.;
RT   "Purification and characterization of the cell surface virulent A protein
RT   from Aeromonas salmonicida.";
RL   Biochemistry 22:2934-2939(1983).
RN   [4]
RP   FUNCTION, AND BINDING PROPERTIES.
RX   PubMed=1730607; DOI=10.1016/s0021-9258(18)48455-4;
RA   Doig P., Emoedy L., Trust T.J.;
RT   "Binding of laminin and fibronectin by the trypsin-resistant major
RT   structural domain of the crystalline virulence surface array protein of
RT   Aeromonas salmonicida.";
RL   J. Biol. Chem. 267:43-49(1992).
CC   -!- FUNCTION: S-layer protein. The S-layer is a paracrystalline mono-
CC       layered assembly of proteins which coats the surface of bacteria. This
CC       S-layer, also called A-layer, confers virulence to the bacterium
CC       infecting salmonid and non-salmonid fish. It binds specifically to
CC       laminin and fibronectin. {ECO:0000269|PubMed:1730607}.
CC   -!- SUBUNIT: Monomer. Forms oligomers at high protein or low salt
CC       concentrations. {ECO:0000269|PubMed:6871174}.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall, S-layer
CC       {ECO:0000269|PubMed:6871174}. Note=This bacterium is covered by a S-
CC       layer with tetragonal symmetry. Tethered to the outer membrane by
CC       interaction with O-side chain of the LPS. {ECO:0000305}.
CC   -!- DOMAIN: This S-layer protein is composed of two distinct structural
CC       domains: a larger mass trypsin-resistant N-terminal, containing the
CC       binding region for extracellular matrix proteins, and a smaller C-
CC       terminal domain with intermediate resistance to trypsin.
CC       {ECO:0000305|PubMed:1869553}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M64655; AAA21941.1; -; Genomic_DNA.
DR   PIR; A39351; A39351.
DR   AlphaFoldDB; P35823; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0030115; C:S-layer; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Cell wall; Direct protein sequencing; S-layer; Secreted; Signal; Virulence.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:6525563,
FT                   ECO:0000269|PubMed:6871174"
FT   CHAIN           22..502
FT                   /note="S-layer protein"
FT                   /id="PRO_0000032626"
FT   VARIANT         26
FT                   /note="S -> G (in strain: A450 and A461)"
FT   VARIANT         35
FT                   /note="T -> N (in strain: A450)"
FT   VARIANT         47
FT                   /note="D -> S (in strain: A400 and A450)"
FT   VARIANT         48
FT                   /note="F -> D (in strain: A400)"
SQ   SEQUENCE   502 AA;  52869 MW;  8B86AC6C4FE46F77 CRC64;
     MFKKTLIAAA IVVGSAAPAF ADVVISPNDN TFVTTSLASV TKQPVLDFST AQQNLTLNFS
     EVGDLKNNGF IVLEIQGEGQ FNDAEIRQWL SNGFWRRPFT GLLVNPNDHG NFANSGEVND
     VRKFFKIISD GTQLTIVHTI DSNGKRLRLA LASDVEETIN FADAEVELKL NLANQAFKLT
     SGSQGTVALT AGALWNASYT ADPVATKPLF KLGKLFQLSL TNAGKATALV SEGFLKLNIG
     DANISATDFA ITNVTTNQTI QRDKVNLTLT GDVSAFKKDA NGNLVNKAGA SIGWKAAADG
     QSATAVLGAG NMAGGVQNAL AAFGTLYVAA DNTVPVPAVN FNVKAEIQGD SQATYNYFKD
     ELADLFILTR DGMKFDTITT GTTSANLIHI RDVSNILPTE GGKIFVTITE YADHAANGRG
     EGTVLVTRKA LSVTLPSGGA VTLKPADVAA DVGASITAGR QARLVFEVET NQGEVAVKKS
     NAEGVDIQNG TRGTAPLVDF TL