SLAP_CAUVC
ID SLAP_CAUVC Reviewed; 1026 AA.
AC P35828; Q46015; Q9RFI2;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 4.
DT 25-MAY-2022, entry version 136.
DE RecName: Full=S-layer protein;
DE AltName: Full=Paracrystalline surface layer protein;
GN Name=rsaA; OrderedLocusNames=CC_1007;
OS Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC Caulobacteraceae; Caulobacter.
OX NCBI_TaxID=190650;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-22; 647-661
RP AND 1022-1026.
RC STRAIN=ATCC 19089 / CB15;
RX PubMed=1393820; DOI=10.1139/m92-033;
RA Gilchrist A., Fisher J.A., Smit J.K.;
RT "Nucleotide sequence analysis of the gene encoding the Caulobacter
RT crescentus paracrystalline surface layer protein.";
RL Can. J. Microbiol. 38:193-202(1992).
RN [2]
RP SEQUENCE REVISION TO 377; 637 AND 843-844.
RA Awram P.;
RL Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=JS3001;
RA Bingle W.H., Awram P.A., Nomellini J.F., Smit J.K.;
RT "The secretion signal of C. crescentus S-layer protein is located in the C-
RT terminal 82 amino acids of the molecule.";
RL Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19089 / CB15;
RX PubMed=11259647; DOI=10.1073/pnas.061029298;
RA Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E.,
RA Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R.,
RA Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B.,
RA DeBoy R.T., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F.,
RA Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R.,
RA Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O.,
RA Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M.;
RT "Complete genome sequence of Caulobacter crescentus.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-314, AND PROTEIN SEQUENCE OF 2-21.
RC STRAIN=ATCC 19089 / CB15;
RX PubMed=3049545; DOI=10.1128/jb.170.10.4706-4713.1988;
RA Fisher J.A., Smit J.K., Agabian N.;
RT "Transcriptional analysis of the major surface array gene of Caulobacter
RT crescentus.";
RL J. Bacteriol. 170:4706-4713(1988).
RN [6]
RP CHARACTERIZATION.
RC STRAIN=ATCC 19089 / CB15;
RX PubMed=9620954; DOI=10.1128/jb.180.12.3062-3069.1998;
RA Awram P., Smit J.K.;
RT "The Caulobacter crescentus paracrystalline S-layer protein is secreted by
RT an ABC transporter (type I) secretion apparatus.";
RL J. Bacteriol. 180:3062-3069(1998).
CC -!- FUNCTION: The S-layer is a paracrystalline mono-layered assembly of
CC proteins which coats the surface of bacteria. Probably acts as a
CC physical barrier to parasites and lytic enzymes.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall, S-layer. Note=This bacterium
CC is covered by a S-layer with hexagonal symmetry. Secreted by an ABC
CC transporter (type I) secretion apparatus.
CC -!- MISCELLANEOUS: The calcium binding of this protein may mediate surface
CC attachment by ionic bridging between the S-layer and a specific
CC membrane-associated oligosaccharide.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK22991.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF062345; AAC38665.2; -; Genomic_DNA.
DR EMBL; AF193063; AAF19365.1; -; Genomic_DNA.
DR EMBL; AE005673; AAK22991.1; ALT_INIT; Genomic_DNA.
DR PIR; A48995; A48995.
DR PIR; C87374; C87374.
DR RefSeq; NP_419823.1; NC_002696.2.
DR RefSeq; WP_024265658.1; NC_002696.2.
DR RefSeq; YP_002516432.2; NC_011916.1.
DR PDB; 5N8P; X-ray; 2.70 A; A/B/C/D/E/F=1-1026.
DR PDB; 6P5T; X-ray; 2.10 A; A/B/C/D/E/F=223-1026.
DR PDB; 6T72; EM; 3.70 A; A=2-250.
DR PDB; 6Z7P; EM; 4.80 A; A=2-1026.
DR PDB; 7PEO; EM; 4.37 A; A=2-250.
DR PDBsum; 5N8P; -.
DR PDBsum; 6P5T; -.
DR PDBsum; 6T72; -.
DR PDBsum; 6Z7P; -.
DR PDBsum; 7PEO; -.
DR AlphaFoldDB; P35828; -.
DR SMR; P35828; -.
DR STRING; 190650.CC_1007; -.
DR EnsemblBacteria; AAK22991; AAK22991; CC_1007.
DR GeneID; 7329809; -.
DR KEGG; ccr:CC_1007; -.
DR KEGG; ccs:CCNA_01059; -.
DR PATRIC; fig|190650.5.peg.1025; -.
DR eggNOG; COG2931; Bacteria.
DR HOGENOM; CLU_267137_0_0_5; -.
DR OMA; VAPHRNT; -.
DR Proteomes; UP000001816; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0030115; C:S-layer; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR DisProt; DP02504; -.
DR Gene3D; 2.150.10.10; -; 1.
DR InterPro; IPR001343; Hemolysn_Ca-bd.
DR InterPro; IPR011049; Serralysin-like_metalloprot_C.
DR Pfam; PF00353; HemolysinCabind; 2.
DR SUPFAM; SSF51120; SSF51120; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Cell wall; Direct protein sequencing;
KW Reference proteome; S-layer; Secreted.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:1393820,
FT ECO:0000269|PubMed:3049545"
FT CHAIN 2..1026
FT /note="S-layer protein"
FT /id="PRO_0000205336"
FT STRAND 247..253
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 260..262
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 265..272
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 274..277
FT /evidence="ECO:0007829|PDB:5N8P"
FT STRAND 278..281
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 286..288
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 295..302
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 312..315
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 318..325
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 327..330
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 332..337
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 340..348
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 350..354
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 359..366
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 372..376
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 378..384
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 386..389
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 392..398
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 404..410
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 412..414
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 419..422
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 425..433
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 440..442
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 446..449
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 456..460
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 473..475
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 479..482
FT /evidence="ECO:0007829|PDB:6P5T"
FT TURN 484..487
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 497..509
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 515..528
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 540..556
FT /evidence="ECO:0007829|PDB:6P5T"
FT TURN 557..561
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 566..572
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 577..583
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 589..596
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 598..604
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 610..613
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 619..624
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 630..632
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 639..642
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 647..650
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 653..655
FT /evidence="ECO:0007829|PDB:5N8P"
FT STRAND 657..661
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 670..672
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 679..682
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 684..687
FT /evidence="ECO:0007829|PDB:6P5T"
FT HELIX 689..691
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 695..697
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 700..703
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 709..713
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 718..722
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 729..734
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 740..742
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 750..755
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 759..761
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 763..772
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 775..780
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 785..797
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 800..806
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 811..824
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 833..835
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 846..849
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 858..862
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 869..871
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 876..881
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 884..886
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 888..891
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 893..899
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 906..909
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 915..917
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 919..922
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 929..931
FT /evidence="ECO:0007829|PDB:6P5T"
FT HELIX 933..935
FT /evidence="ECO:0007829|PDB:6P5T"
FT HELIX 958..965
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 971..973
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 975..981
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 984..990
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 992..996
FT /evidence="ECO:0007829|PDB:6P5T"
FT TURN 999..1001
FT /evidence="ECO:0007829|PDB:5N8P"
FT STRAND 1002..1009
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 1017..1019
FT /evidence="ECO:0007829|PDB:6P5T"
FT STRAND 1022..1024
FT /evidence="ECO:0007829|PDB:6P5T"
SQ SEQUENCE 1026 AA; 98133 MW; AD7DB818D7C528AC CRC64;
MAYTTAQLVT AYTNANLGKA PDAATTLTLD AYATQTQTGG LSDAAALTNT LKLVNSTTAV
AIQTYQFFTG VAPSAAGLDF LVDSTTNTND LNDAYYSKFA QENRFINFSI NLATGAGAGA
TAFAAAYTGV SYAQTVATAY DKIIGNAVAT AAGVDVAAAV AFLSRQANID YLTAFVRANT
PFTAAADIDL AVKAALIGTI LNAATVSGIG GYATATAAMI NDLSDGALST DNAAGVNLFT
AYPSSGVSGS TLSLTTGTDT LTGTANNDTF VAGEVAGAAT LTVGDTLSGG AGTDVLNWVQ
AAAVTALPTG VTISGIETMN VTSGAAITLN TSSGVTGLTA LNTNTSGAAQ TVTAGAGQNL
TATTAAQAAN NVAVDGGANV TVASTGVTSG TTTVGANSAA SGTVSVSVAN SSTTTTGAIA
VTGGTAVTVA QTAGNAVNTT LTQADVTVTG NSSTTAVTVT QTAAATAGAT VAGRVNGAVT
ITDSAAASAT TAGKIATVTL GSFGAATIDS SALTTVNLSG TGTSLGIGRG ALTATPTANT
LTLNVNGLTT TGAITDSEAA ADDGFTTINI AGSTASSTIA SLVAADATTL NISGDARVTI
TSHTAAALTG ITVTNSVGAT LGAELATGLV FTGGAGADSI LLGATTKAIV MGAGDDTVTV
SSATLGAGGS VNGGDGTDVL VANVNGSSFS ADPAFGGFET LRVAGAAAQG SHNANGFTAL
QLGATAGATT FTNVAVNVGL TVLAAPTGTT TVTLANATGT SDVFNLTLSS SAALAAGTVA
LAGVETVNIA ATDTNTTAHV DTLTLQATSA KSIVVTGNAG LNLTNTGNTA VTSFDASAVT
GTGSAVTFVS ANTTVGEVVT IRGGAGADSL TGSATANDTI IGGAGADTLV YTGGTDTFTG
GTGADIFDIN AIGTSTAFVT ITDAAVGDKL DLVGISTNGA IADGAFGAAV TLGAAATLAQ
YLDAAAAGDG SGTSVAKWFQ FGGDTYVVVD SSAGATFVSG ADAVIKLTGL VTLTTSAFAT
EVLTLA
