ID   SLAP_LACAC              Reviewed;         444 AA.
AC   P35829; Q5FMK4;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=S-layer protein;
DE   AltName: Full=Surface layer protein;
DE            Short=SA-protein;
DE   Flags: Precursor;
GN   Name=slpA; OrderedLocusNames=LBA0169;
OS   Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=272621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 343-351 AND
RP   440-444.
RC   STRAIN=ATCC 4356 / DSM 20079 / NBRC 13951 / JCM 1132 / NCIMB 8690;
RX   PubMed=8407780; DOI=10.1128/jb.175.19.6089-6096.1993;
RA   Boot H.J., Kolen C.P.A.M., van Noort J.M., Pouwels P.H.;
RT   "S-layer protein of Lactobacillus acidophilus ATCC 4356: purification,
RT   expression in Escherichia coli, and nucleotide sequence of the
RT   corresponding gene.";
RL   J. Bacteriol. 175:6089-6096(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 4356 / DSM 20079 / NBRC 13951 / JCM 1132 / NCIMB 8690;
RX   PubMed=8522531; DOI=10.1128/jb.177.24.7222-7230.1995;
RA   Boot H.J., Kolen C.P.A.M., Pouwels P.H.;
RT   "Identification, cloning, and nucleotide sequence of a silent S-layer
RT   protein gene of Lactobacillus acidophilus ATCC 4356 which has extensive
RT   similarity with the S-layer protein gene of this species.";
RL   J. Bacteriol. 177:7222-7230(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700396 / NCK56 / N2 / NCFM;
RX   PubMed=15671160; DOI=10.1073/pnas.0409188102;
RA   Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L.,
RA   McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S.,
RA   Hamrick A., Cano R., Klaenhammer T.R.;
RT   "Complete genome sequence of the probiotic lactic acid bacterium
RT   Lactobacillus acidophilus NCFM.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005).
CC   -!- FUNCTION: The S-layer is a paracrystalline mono-layered assembly of
CC       proteins which coat the surface of bacteria.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall, S-layer. Note=This bacterium
CC       is covered by a S-layer with hexagonal symmetry.
CC   -!- PTM: Glycosylated.
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DR   EMBL; X71412; CAA50535.1; -; Genomic_DNA.
DR   EMBL; X89375; CAA61560.1; -; Genomic_DNA.
DR   EMBL; CP000033; AAV42070.1; -; Genomic_DNA.
DR   PIR; A36924; A36924.
DR   RefSeq; WP_011254065.1; NC_006814.3.
DR   RefSeq; YP_193101.1; NC_006814.3.
DR   AlphaFoldDB; P35829; -.
DR   DIP; DIP-60786N; -.
DR   IntAct; P35829; 1.
DR   PRIDE; P35829; -.
DR   EnsemblBacteria; AAV42070; AAV42070; LBA0169.
DR   GeneID; 56941778; -.
DR   KEGG; lac:LBA0169; -.
DR   PATRIC; fig|272621.13.peg.165; -.
DR   eggNOG; ENOG50300QQ; Bacteria.
DR   HOGENOM; CLU_027196_0_0_9; -.
DR   OMA; DYSHINL; -.
DR   BioCyc; LACI272621:G1G49-167-MON; -.
DR   Proteomes; UP000006381; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; IEA:InterPro.
DR   GO; GO:0030115; C:S-layer; IEA:UniProtKB-SubCell.
DR   GO; GO:0005199; F:structural constituent of cell wall; IEA:InterPro.
DR   InterPro; IPR004903; S-layer_prot.
DR   InterPro; IPR024968; SlpA_dom.
DR   Pfam; PF03217; SlpA; 2.
DR   PIRSF; PIRSF037863; SLAP; 1.
DR   PRINTS; PR01729; SURFACELAYER.
PE   1: Evidence at protein level;
KW   Cell wall; Direct protein sequencing; Glycoprotein; Reference proteome;
KW   S-layer; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..444
FT                   /note="S-layer protein"
FT                   /id="PRO_0000032637"
SQ   SEQUENCE   444 AA;  46570 MW;  2090732F89099161 CRC64;
     MKKNLRIVSA AAAALLAVAP VAASAVSTVS AATTINASSS AINTNTNAKY DVDVTPSVSA
     VAANTANNTP AIAGNLTGTI SASYNGKTYT ANLKADTENA TITAAGSTTA VKPAELAAGV
     AYTVTVNDVS FNFGSENAGK TVTLGSANSN VKFTGTNSDN QTETNVSTLK VKLDQNGVAS
     LTNVSIANVY AINTTDNSNV NFYDVTSGAT VTNGAVSVNA DNQGQVNVAN VVAAINSKYF
     AAQYADKKLN TRTANTEDAI KAALKDQKID VNSVGYFKAP HTFTVNVKAT SNTNGKSATL
     PVVVTVPNVA EPTVASVSKR IMHNAYYYDK DAKRVGTDSV KRYNSVSVLP NTTTINGKTY
     YQVVENGKAV DKYINAANID GTKRTLKHNA YVYASSKKRA NKVVLKKGEV VTTYGASYTF
     KNGQKYYKIG DNTDKTYVKV ANFR