ID   SLAP_LYSSH              Reviewed;        1176 AA.
AC   P38537;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Surface-layer 125 kDa protein;
DE   Flags: Precursor;
OS   Lysinibacillus sphaericus (Bacillus sphaericus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lysinibacillus.
OX   NCBI_TaxID=1421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=2362;
RX   PubMed=2666389; DOI=10.1128/jb.171.8.4178-4188.1989;
RA   Bowditch R.D., Baumann P., Yousten A.A.;
RT   "Cloning and sequencing of the gene encoding a 125-kilodalton surface-layer
RT   protein from Bacillus sphaericus 2362 and of a related cryptic gene.";
RL   J. Bacteriol. 171:4178-4188(1989).
CC   -!- FUNCTION: The S-layer is a paracrystalline mono-layered assembly of
CC       proteins which coat the surface of bacteria.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall, S-layer.
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DR   EMBL; M28361; AAA50256.1; -; Genomic_DNA.
DR   PIR; A33856; A33856.
DR   RefSeq; WP_012292791.1; NZ_LWHI01000001.1.
DR   AlphaFoldDB; P38537; -.
DR   SMR; P38537; -.
DR   STRING; 1421.A2J09_21570; -.
DR   OMA; DYVYAEQ; -.
DR   OrthoDB; 11115at2; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0030115; C:S-layer; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.60.40.1220; -; 5.
DR   InterPro; IPR014755; Cu-Rt/internalin_Ig-like.
DR   InterPro; IPR001119; SLH_dom.
DR   Pfam; PF00395; SLH; 3.
DR   PROSITE; PS51272; SLH; 3.
PE   3: Inferred from homology;
KW   Cell wall; Repeat; S-layer; Secreted; Signal.
FT   SIGNAL          1..30
FT                   /evidence="ECO:0000255"
FT   CHAIN           31..1176
FT                   /note="Surface-layer 125 kDa protein"
FT                   /id="PRO_0000032631"
FT   DOMAIN          31..88
FT                   /note="SLH 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT   DOMAIN          89..152
FT                   /note="SLH 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT   DOMAIN          153..216
FT                   /note="SLH 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
SQ   SEQUENCE   1176 AA;  125226 MW;  1A9A458EF433788C CRC64;
     MAKQNKGRKF FAASATAALV ASAIVPVASA AQLNDFNKIS GYAKEAVQSL VDAGVIQGDA
     NGNFNPLKTI SRAEAATIFT NALELEAEGD VNFKDVKADA WYYDAIAATV ENGIFEGVSA
     TEFAPNKQLT RSEAAKILVD AFELEGEGDL SEFADASTVK PWAKSYLEIA VANGVIKGSE
     ANGKTNLNPN APITRQDFAV VFSRTIENVD ATPKVDKIEV VDAKTLNVTL SDGTKETVTL
     EKALEPNKET EVTFKIKDVE YKAKVTYVVT TATAVKSVSA TNLKEVVVEF DGTVDKETAE
     DAANYALKSG KTIKSVSLAA DNKTATVTLT DKLNNNKADA ISISNVKAGD KEINVKNVEF
     TAVDNKIPEV TEVKSLGTKA VKVTLSEPVE NLSSTNFTLD GKAYFGNVVM GAGNKTVILT
     PYSSSALSVG DHKLTVSGAK DFAGFVSLNS THEFKVVEDK EAPTVTEATA TLETVTLTFS
     EDIDMDTVKA SNVYWKSGDS KKEASEFERI ADNKYKFVFK GSEKTLPTGK VDVYVEDIKD
     YSDNKIAKDT KVTVTPEIDQ TRPEVRKVTA LDEKTIKVTF SKTVDGESAI KTGNYTVKDK
     DDKVVSVDKV TVDSKDSKSV IIDLYSKVSV GENTITIKNV KDATKLNNTM LDYTGKFTRS
     DKEGPDYEHV INADAKAKKV VLKFDKKMDA ASLADYSNYL VKINDTLQTL SEDVATLSVS
     NDATVVTITF AETIKGDDVV FASGKAISGS GKVNVNELQV MGVKDTSGNV HKKFNGSENK
     ITLSSTSTPL KLAKIDKDYD AKYTAELVDR KTVKVKFSTV INSAAANAFT SESHKIDSIQ
     VNGTSTVTVK FKDEINTNAS DLDLKVNLSK LVDIAGNEST NNTPIAIKAG INLLDSVAPV
     VVGEPVVDKE TITFTFSENL TSVSIGEVLS TDFTVTRVSD NKDLAIKDYS VAIANNNQVV
     ITLSDNREVA TAYKVTAKNA KLITDDNGDK KNAIADFTKT TATKVEASGT LSLDAAKTNL
     NNEITKAKDA KATGTEGTAA TNQIVGSKDA LQVAIDVAEL VKNDTAATLQ QLTDAKTDLT
     AAITAYNAAK VEDISSLLVA PDLVLGTTDN GTITGFVAGT GETLKVTSDS AANVEVTDPT
     GLAVTAKAKG EANILVQVLK GDKVIKTGTV KVTVSE