ID   SLAP_THEKI              Reviewed;         762 AA.
AC   P22258;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Cell surface protein;
DE   AltName: Full=S-layer protein;
DE   Flags: Precursor;
OS   Thermoanaerobacter kivui (Acetogenium kivui).
OC   Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC   Thermoanaerobacteraceae; Thermoanaerobacter.
OX   NCBI_TaxID=2325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=ATCC 33488 / DSM 2030 / LKT-1;
RX   PubMed=2681162; DOI=10.1128/jb.171.11.6307-6315.1989;
RA   Peters J., Peters M., Lottspeich F., Baumeister W.;
RT   "S-layer protein gene of Acetogenium kivui: cloning and expression in
RT   Escherichia coli and determination of the nucleotide sequence.";
RL   J. Bacteriol. 171:6307-6315(1989).
RN   [2]
RP   PARTIAL PROTEIN SEQUENCE, AND GLYCOSYLATION AT TYR-297; TYR-516; TYR-520
RP   AND TYR-632.
RC   STRAIN=ATCC 33488 / DSM 2030 / LKT-1;
RX   PubMed=1596358; DOI=10.1515/bchm3.1992.373.1.171;
RA   Peters J., Rudolf S., Oschkinat H., Mengele R., Sumper M., Kellermann J.,
RA   Lottspeich F., Baumeister W.;
RT   "Evidence for tyrosine-linked glycosaminoglycan in a bacterial surface
RT   protein.";
RL   Biol. Chem. Hoppe-Seyler 373:171-176(1992).
RN   [3]
RP   DOMAINS.
RX   PubMed=8113161; DOI=10.1128/jb.176.5.1224-1233.1994;
RA   Lupas A., Engelhardt H., Peters J., Santarius U., Volker S., Baumeister W.;
RT   "Domain structure of the Acetogenium kivui surface layer revealed by
RT   electron crystallography and sequence analysis.";
RL   J. Bacteriol. 176:1224-1233(1994).
CC   -!- FUNCTION: The S-layer is a paracrystalline mono-layered assembly of
CC       proteins which coat the surface of bacteria.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall, S-layer. Note=This bacterium
CC       is covered by a S-layer with hexagonal symmetry.
CC   -!- PTM: Glycosylated; contains 8% carbohydrates, which correspond to about
CC       40 to 50 sugar molecules per monomer. O-linked glycans consist of Glc,
CC       GalNAc and GlcNAc. {ECO:0000269|PubMed:1596358}.
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DR   EMBL; M31069; AAA21930.1; -; Genomic_DNA.
DR   PIR; A34355; A34355.
DR   AlphaFoldDB; P22258; -.
DR   SMR; P22258; -.
DR   STRING; 2325.TKV_c23170; -.
DR   eggNOG; COG2373; Bacteria.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0030115; C:S-layer; IEA:UniProtKB-SubCell.
DR   InterPro; IPR001119; SLH_dom.
DR   Pfam; PF00395; SLH; 2.
DR   PROSITE; PS51272; SLH; 2.
PE   1: Evidence at protein level;
KW   Cell wall; Direct protein sequencing; Glycoprotein; Repeat; S-layer;
KW   Secreted; Signal.
FT   SIGNAL          1..26
FT   CHAIN           27..762
FT                   /note="Cell surface protein"
FT                   /id="PRO_0000032625"
FT   DOMAIN          27..90
FT                   /note="SLH 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT   DOMAIN          92..155
FT                   /note="SLH 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT   DOMAIN          156..204
FT                   /note="SLH 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT   CARBOHYD        297
FT                   /note="O-linked (Glc...) tyrosine"
FT                   /evidence="ECO:0000269|PubMed:1596358"
FT   CARBOHYD        516
FT                   /note="O-linked (Glc...) tyrosine"
FT                   /evidence="ECO:0000269|PubMed:1596358"
FT   CARBOHYD        520
FT                   /note="O-linked (Glc...) tyrosine"
FT                   /evidence="ECO:0000269|PubMed:1596358"
FT   CARBOHYD        632
FT                   /note="O-linked (Glc...) tyrosine"
FT                   /evidence="ECO:0000269|PubMed:1596358"
SQ   SEQUENCE   762 AA;  82785 MW;  34EC9C784DECA67E CRC64;
     MKNLKKLIAV VSTFALVFSA MAVGFAATTP FTDVKDDAPY ASAVARLYAL NITNGVGDPK
     FGVDQPVTRA QMITFVNRML GYEDLAEMAK SEKSAFKDVP QNHWAVGQIN LAYKLGLAQG
     VGNGKFDPNS ELRYAQALAF VLRALGFKDL DWPYGYLAKA QDLGLVHGLN LAYNGVIKRG
     DLALILDRAL EVPMVKYVDG KEVLGEPLIS KVATKAEYTV IATNAQDRSV EEGKVAVLDK
     DGKLTTINAG LVDFSEYLGK KVIVYSERFG DPVYVAEGDN DVVSFTEGQD SVGTTVYKND
     DNKTAIKVDD NAYVLYNGYL TKVSKVTVKE GAEVTIINNN YLIVNGSYDN STIVYNDVQS
     GDKYLNRDSN YELKGTVTVT GAVSKVTDIK ANDYIYYGKQ YDVNGNVVGT VIYVVRNQVT
     GTVTEKSVSG STYKASIDNV SYTVADNNVW NQLEPGKKVT VILNKDNVIV GISSTTTTTA
     VNYAIFKEKS DPFTAWFAKV KLILPDAAEK VFDAVYSDVY DKVNLAEGTI VTYTVDANGK
     LNDIQRANDQ PFSSASYKAD AKVLTEGSTT YYITDNTVLL NNTSDGYKAL KLTDLKDATN
     LNVKIVADNY NVAKVVVFNN ASFVSTTTST VYAYVTGTAD VYVNGSTFTR LTVLENGQTK
     TYDANAQLAT NYTHKAVVLT LTNAKIANIA LPTVASGVKL TNIDQANLRI TDTTNKGYLL
     DPNFIVVDTN GNLKGLSDIT KDTGVNLYTN DVGKVFVIEI VQ