ID   BHUA_BRUA2              Reviewed;         661 AA.
AC   Q2YJB2;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Heme transporter BhuA;
DE   AltName: Full=Brucella heme uptake protein A;
DE   Flags: Precursor;
GN   Name=bhuA; OrderedLocusNames=BAB2_1150;
OS   Brucella abortus (strain 2308).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=359391;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2308;
RX   PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA   Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA   Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT   "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL   Infect. Immun. 73:8353-8361(2005).
RN   [2]
RP   FUNCTION IN HEME TRANSPORT, AND DISRUPTION PHENOTYPE.
RX   PubMed=17709407; DOI=10.1128/iai.00460-07;
RA   Paulley J.T., Anderson E.S., Roop R.M. II;
RT   "Brucella abortus requires the heme transporter BhuA for maintenance of
RT   chronic infection in BALB/c mice.";
RL   Infect. Immun. 75:5248-5254(2007).
CC   -!- FUNCTION: Heme transporter playing an important role in stationary-
CC       phase iron acquisition and required for maintenance of chronic
CC       infection in mice. {ECO:0000269|PubMed:17709407}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}.
CC   -!- INDUCTION: Induced in absence of iron. Maximally expressed during
CC       stationary phase when cultivated in low-iron minimal medium.
CC   -!- DISRUPTION PHENOTYPE: The mutant is unable to use hemin as an iron
CC       source but retains the ability to use ferric chloride and the
CC       siderophore 2,3-DHBA. Exhibits an accelerated loss of viability after
CC       144 hours of culture in low-iron minimal medium. Exhibits significant
CC       attenuation in murine macrophages and is unable to maintain a chronic
CC       spleen infection in mice. {ECO:0000269|PubMed:17709407}.
CC   -!- SIMILARITY: Belongs to the TonB-dependent receptor family.
CC       {ECO:0000305}.
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DR   EMBL; AM040265; CAJ13316.1; -; Genomic_DNA.
DR   RefSeq; WP_006099340.1; NZ_KN046823.1.
DR   AlphaFoldDB; Q2YJB2; -.
DR   SMR; Q2YJB2; -.
DR   STRING; 359391.BAB2_1150; -.
DR   TCDB; 1.B.14.2.10; the outer membrane receptor (omr) family.
DR   PRIDE; Q2YJB2; -.
DR   EnsemblBacteria; CAJ13316; CAJ13316; BAB2_1150.
DR   GeneID; 3828017; -.
DR   KEGG; bmf:BAB2_1150; -.
DR   PATRIC; fig|359391.11.peg.1936; -.
DR   HOGENOM; CLU_008287_19_4_5; -.
DR   OMA; KFMDESA; -.
DR   PhylomeDB; Q2YJB2; -.
DR   Proteomes; UP000002719; Chromosome II.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   Gene3D; 2.170.130.10; -; 1.
DR   Gene3D; 2.40.170.20; -; 1.
DR   InterPro; IPR039426; BtuB-like.
DR   InterPro; IPR012910; Plug_dom.
DR   InterPro; IPR037066; Plug_dom_sf.
DR   InterPro; IPR000531; TonB-dep_rcpt_b-brl.
DR   InterPro; IPR036942; TonB_rcpt_b-brl_sf.
DR   PANTHER; PTHR30069; PTHR30069; 1.
DR   Pfam; PF07715; Plug; 1.
DR   Pfam; PF00593; TonB_dep_Rec; 1.
PE   1: Evidence at protein level;
KW   Cell outer membrane; Membrane; Receptor; Reference proteome; Signal;
KW   TonB box; Transmembrane; Transmembrane beta strand.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..661
FT                   /note="Heme transporter BhuA"
FT                   /id="PRO_0000325857"
SQ   SEQUENCE   661 AA;  72931 MW;  4EF2DE0C6CE74C7B CRC64;
     MKFTRTLVLV STSLLATVAT SQAQEVKRDT KKQGEVVLKP ITIISHGKDN IEATGGTVLT
     YKDIEKLQPA NVSELFSRQS SIAVSGGGGP SKRIHVLGME QSNLAVSVDG VPQTATSWHH
     TGSNVIDPAF LKRVEVEAGA AAADSGFGAA AGAIRYETVN ALDLLEPGKT FGARIIGSYG
     TNGRGFSGST AAYGLKDGFD WLLMLHGTSG HNYKNGDGTE ILGTEPAARN ILGKAGYEFD
     GNRIDIGYER SRDKADRLIK MNMGLPGDTE YPLEVARDSV NIKYTRTDAT DMWDPEVQFY
     YNRNDYWRND YQNRTNGNMI LKEDLYGGKL QNTFTIDYGK ITAGIDFGKH DYNTDNYGHN
     DRRYRKFNTQ QVGAFTQGRF EFDNGFSLST GARYDYSRFA DWNDEVFSDS GASVNGTLSY
     KFNEHIEVFA GASRTWLGYV LGDYGYVHAR NNAFYTDPTF SPGRARNYKA GVNFGGADWS
     AGITLFDTRI AGLPNYDSQK LGNDPEEYRS RGFTLNARYI WNYTTIGATF TKAKVTAGDD
     PVLPNSGSFM PIGDMATLFI DQEIPDYNMK VGATLAWAGR ISDEAATAAN FYDQPAYTVV
     NAYAEWNPPA VKNMTLRVGV ENLFNENYYE RTSFAPSQNR GGIDAVWAPG RTFTFQTAFK
     F