SLBB_TRIAB
ID SLBB_TRIAB Reviewed; 146 AA.
AC P81116; A7LAC9; Q9PS20;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 2.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Snaclec alboaggregin-B subunit beta;
DE Short=AL-B subunit beta;
DE AltName: Full=25-kDa alboaggregin;
DE Flags: Precursor;
OS Trimeresurus albolabris (White-lipped pit viper) (Cryptelytrops
OS albolabris).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Viperidae; Crotalinae; Trimeresurus.
OX NCBI_TaxID=8765;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT LYS-51.
RX PubMed=17538847; DOI=10.1080/09537100601078232;
RA Arpijuntarangkoon J., Rojnuckarin P., Muanpasitporn C., Kaeothip S.,
RA Sangvanich P., Intragumtornchai T.;
RT "Molecular cloning and sequence analysis of alboaggregin B.";
RL Platelets 18:266-272(2007).
RN [2]
RP PROTEIN SEQUENCE OF 24-146.
RX PubMed=8645249; DOI=10.1006/bbrc.1996.0302;
RA Usami Y., Suzuki M., Yoshida E., Sakurai Y., Hirano K., Kawasaki T.,
RA Fujimura Y., Titani K.;
RT "Primary structure of alboaggregin-B purified from the venom of
RT Trimeresurus albolabris.";
RL Biochem. Biophys. Res. Commun. 219:727-733(1996).
RN [3]
RP PROTEIN SEQUENCE OF 24-141.
RC TISSUE=Venom;
RX PubMed=9531050;
RA Kowalska M.A., Tan L., Holt J.C., Peng M., Karczewski J., Calvete J.J.,
RA Niewiarowski S.;
RT "Alboaggregins A and B. Structure and interaction with human platelets.";
RL Thromb. Haemost. 79:609-613(1998).
RN [4]
RP PROTEIN SEQUENCE OF 24-63.
RC TISSUE=Venom;
RX PubMed=8466514; DOI=10.1006/bbrc.1993.1371;
RA Yoshida E., Fujimura Y., Miura S., Sugimoto M., Fukui H., Narita N.,
RA Usami Y., Suzuki M., Titani K.;
RT "Alboaggregin-B and botrocetin, two snake venom proteins with highly
RT homologous amino acid sequences but totally distinct functions on von
RT Willebrand factor binding to platelets.";
RL Biochem. Biophys. Res. Commun. 191:1386-1392(1993).
RN [5]
RP FUNCTION.
RC TISSUE=Venom;
RX PubMed=1747371; DOI=10.1021/bi00113a007;
RA Peng M., Lu W., Kirby E.P.;
RT "Alboaggregin-B: a new platelet agonist that binds to platelet membrane
RT glycoprotein Ib.";
RL Biochemistry 30:11529-11536(1991).
CC -!- FUNCTION: Weakly agglutinates platelets at high doses by binding to
CC GPIbalpha (GP1BA). {ECO:0000269|PubMed:1747371}.
CC -!- SUBUNIT: Heterodimer of subunits alpha and beta; disulfide-linked.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC -!- MISCELLANEOUS: Monoclonal antibodies to glycoprotein IIb/IIIa, to
CC bovine vWF, and to bovine serum albumin did not show any effect on the
CC binding of AL-B to platelets. {ECO:0000305|PubMed:1747371}.
CC -!- SIMILARITY: Belongs to the snaclec family. {ECO:0000305}.
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DR EMBL; EF690368; ABS12077.1; -; mRNA.
DR PIR; A56829; A56829.
DR AlphaFoldDB; P81116; -.
DR SMR; P81116; -.
DR MEROPS; I63.002; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR Gene3D; 3.10.100.10; -; 1.
DR InterPro; IPR001304; C-type_lectin-like.
DR InterPro; IPR016186; C-type_lectin-like/link_sf.
DR InterPro; IPR016187; CTDL_fold.
DR Pfam; PF00059; Lectin_C; 1.
DR SMART; SM00034; CLECT; 1.
DR SUPFAM; SSF56436; SSF56436; 1.
DR PROSITE; PS50041; C_TYPE_LECTIN_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Hemostasis impairing toxin;
KW Platelet aggregation activating toxin; Secreted; Signal; Toxin.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..146
FT /note="Snaclec alboaggregin-B subunit beta"
FT /id="PRO_0000046713"
FT DOMAIN 24..143
FT /note="C-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 25..36
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 53..142
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 98
FT /note="Interchain (with C-79 in subunit alpha)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 119..134
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT VARIANT 51
FT /note="Q -> K"
FT /evidence="ECO:0000269|PubMed:17538847"
FT CONFLICT 38
FT /note="K -> R (in Ref. 2; AA sequence and 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 42..44
FT /note="QRM -> EKK (in Ref. 2; AA sequence and 4; AA
FT sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 51
FT /note="Q -> K (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 54
FT /note="R -> T (in Ref. 2; AA sequence and 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 59
FT /note="G -> D (in Ref. 2; AA sequence and 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 62
FT /note="L -> I (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 63
FT /note="L -> V (in Ref. 2; AA sequence and 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 66
FT /note="H -> D (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 71
FT /note="V -> A (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 75
FT /note="V -> A (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 79..86
FT /note="LPILKADF -> FPVLKHDL (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 92..93
FT /note="TD -> GS (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 96
FT /note="S -> N (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 99
FT /note="R -> K (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 107..110
FT /note="ELKY -> TLSK (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 114
FT /note="T -> S (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 121
FT /note="A -> T (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 124
FT /note="T -> S (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 128..130
FT /note="QWW -> EWL (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 144..146
FT /note="LEV -> FQA (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 146 AA; 16871 MW; 84E49D70306C1731 CRC64;
MGRFIFGSFG LLVLFLSLSG TGADCPSDWS SYDLYCYKVF QQRMNWEDAE QFCRQQHTGS
HLLSFHSSEE VDFVVSKTLP ILKADFVWIG LTDVWSACRL QWSDGTELKY NAWTAESECI
ASKTTDNQWW TRSCSRTYPF VCKLEV
