SLC31_RAT
ID SLC31_RAT Reviewed; 683 AA.
AC Q64319; Q62672;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Neutral and basic amino acid transport protein rBAT;
DE AltName: Full=D2;
DE AltName: Full=Solute carrier family 3 member 1;
DE AltName: Full=b(0,+)-type amino acid transport protein;
DE Short=NAA-TR;
GN Name=Slc3a1; Synonyms=Nbat;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Sprague-Dawley; TISSUE=Kidney cortex;
RX PubMed=1376924; DOI=10.1073/pnas.89.12.5596;
RA Wells R.G., Hediger M.A.;
RT "Cloning of a rat kidney cDNA that stimulates dibasic and neutral amino
RT acid transport and has sequence similarity to glucosidases.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:5596-5600(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Kidney;
RX PubMed=1729674; DOI=10.1073/pnas.89.1.1;
RA Tate S.S., Yan N., Udenfriend S.;
RT "Expression cloning of a Na(+)-independent neutral amino acid transporter
RT from rat kidney.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:1-5(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE OF 1-140.
RC STRAIN=Sprague-Dawley; TISSUE=Liver;
RX PubMed=8052618; DOI=10.1073/pnas.91.16.7548;
RA Yan N., Mosckovitz R., Gerber L.D., Mathew S., Murty V.V.V.S., Tate S.S.,
RA Udenfriend S.;
RT "Characterization of the promoter region of the gene for the rat neutral
RT and basic amino acid transporter and chromosomal localization of the human
RT gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:7548-7552(1994).
RN [5]
RP SUBUNIT, AND TISSUE SPECIFICITY.
RX PubMed=10506124; DOI=10.1074/jbc.274.41.28845;
RA Chairoungdua A., Segawa H., Kim J.Y., Miyamoto K., Haga H., Fukui Y.,
RA Mizoguchi K., Ito H., Takeda E., Endou H., Kanai Y.;
RT "Identification of an amino acid transporter associated with the
RT cystinuria-related type II membrane glycoprotein.";
RL J. Biol. Chem. 274:28845-28848(1999).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10 AND SER-383, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Involved in the high-affinity sodium-independent transport of
CC cystine and neutral and dibasic amino acids (system B(0,+)-like
CC activity). May function as an activator of SLC7A9 and be involved in
CC the high-affinity reabsorption of cystine in the kidney proximal
CC tubule. {ECO:0000269|PubMed:1376924, ECO:0000269|PubMed:1729674}.
CC -!- SUBUNIT: Disulfide-linked heterodimer with the amino acid transporter
CC SLC7A9. {ECO:0000269|PubMed:10506124}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Single-pass type II
CC membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in kidney and intestine. In
CC kidney localized to the apical membrane of the proximal tubules.
CC {ECO:0000269|PubMed:10506124, ECO:0000269|PubMed:1376924,
CC ECO:0000269|PubMed:1729674}.
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DR EMBL; M80804; AAA73144.1; -; mRNA.
DR EMBL; M77345; AAA41544.1; -; mRNA.
DR EMBL; BC078852; AAH78852.1; -; mRNA.
DR EMBL; U10110; AAA20394.1; -; Unassigned_DNA.
DR PIR; A41785; A41785.
DR RefSeq; NP_058912.1; NM_017216.1.
DR AlphaFoldDB; Q64319; -.
DR SMR; Q64319; -.
DR STRING; 10116.ENSRNOP00000009581; -.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR GlyGen; Q64319; 7 sites.
DR iPTMnet; Q64319; -.
DR PhosphoSitePlus; Q64319; -.
DR PaxDb; Q64319; -.
DR PRIDE; Q64319; -.
DR Ensembl; ENSRNOT00000009581; ENSRNOP00000009581; ENSRNOG00000007006.
DR GeneID; 29484; -.
DR KEGG; rno:29484; -.
DR UCSC; RGD:3709; rat.
DR CTD; 6519; -.
DR RGD; 3709; Slc3a1.
DR eggNOG; KOG0471; Eukaryota.
DR GeneTree; ENSGT00940000158103; -.
DR HOGENOM; CLU_006462_8_0_1; -.
DR InParanoid; Q64319; -.
DR OMA; PNGEKWA; -.
DR OrthoDB; 1384693at2759; -.
DR PhylomeDB; Q64319; -.
DR TreeFam; TF314498; -.
DR Reactome; R-RNO-352230; Amino acid transport across the plasma membrane.
DR PRO; PR:Q64319; -.
DR Proteomes; UP000002494; Chromosome 6.
DR Bgee; ENSRNOG00000007006; Expressed in kidney and 20 other tissues.
DR Genevisible; Q64319; RN.
DR GO; GO:0016324; C:apical plasma membrane; ISO:RGD.
DR GO; GO:0031526; C:brush border membrane; ISO:RGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:RGD.
DR GO; GO:0005886; C:plasma membrane; IDA:RGD.
DR GO; GO:0005774; C:vacuolar membrane; IDA:RGD.
DR GO; GO:0046982; F:protein heterodimerization activity; ISO:RGD.
DR GO; GO:0044877; F:protein-containing complex binding; IDA:RGD.
DR GO; GO:0006865; P:amino acid transport; IDA:RGD.
DR GO; GO:0015810; P:aspartate transmembrane transport; ISO:RGD.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0010467; P:gene expression; ISO:RGD.
DR GO; GO:0015811; P:L-cystine transport; ISO:RGD.
DR GO; GO:0015813; P:L-glutamate transmembrane transport; ISO:RGD.
DR Gene3D; 2.60.40.1180; -; 1.
DR Gene3D; 3.90.400.10; -; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045857; O16G_dom_2.
DR Pfam; PF00128; Alpha-amylase; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Disulfide bond; Glycoprotein; Membrane;
KW Phosphoprotein; Reference proteome; Signal-anchor; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..683
FT /note="Neutral and basic amino acid transport protein rBAT"
FT /id="PRO_0000071951"
FT TOPO_DOM 1..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..683
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 10
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 383
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT CARBOHYD 211
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 238
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 258
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 329
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 510
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 520
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 574
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 683 AA; 78507 MW; 201C1F2A2F404311 CRC64;
MNEDKDKRDS IQMSMKGCRT NNGFVQNEDI QEQDPDSRDT PQSNAVSIPA PEEPQLKVVR
PYAGMPKEVL FQFSGQARYR VPREILFWLT VVSVFLLIGA TIAIIIISPK CLDWWQAGPM
YQIYPRSFKD SDKDGNGDLK GIQEKLDYIT ALNIKTIWIT SFYKSPLKDF RYAVEDFKEI
DPIFGTMKDF ENLVAAVHDK GLKLIIDFIP NHTSDKHPWF QSSRTRSGKY TDYYIWHNCT
HANGVTTPPN NWLSVYGNSS WQFDEERKQC YFHQFLKEQP DLNFRNPAVQ EEIKEIIKFW
LSKGVDGFSF DAVKFLLEAK DLRNEIQVNT SQIPDTVTRY SELYHDFTTT QVGMHDLVRD
FRQTMNQFSR EPGRYRFMGT EVSAESTERT MVYYGLSFIQ EADFPFNKYL ATLDTLSGHT
VYEAITSWME NMPEGKWPNW MIGGPETSRL TSRVGSEYVN AMNMLLFTLP GTPITYYGEE
IGMGDISITN LNERYDTNAL LSKSPMQWDN SSNAGFTEAN HTWLPTNSDY HTVNVDVQKT
QPSSALRLYQ DLSLLHAREL LLSRGWFCLL RDDNHSVVYT RELDGIDKVF LVVLNFGESS
TVLNLQETIS DVPTKLRIRL STNPASKGSD VDTHAVSLEK GEGLILEHSM KTLLHHQKAF
RDKCFISNRA CYSSVLDLLY SSC
