SLCA_DEIAC
ID SLCA_DEIAC Reviewed; 155 AA.
AC Q9DEA2; Q8JIV6;
DT 25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 66.
DE RecName: Full=Snaclec agkicetin-C subunit alpha;
DE AltName: Full=Antithrombin A subunit A;
DE Flags: Precursor;
OS Deinagkistrodon acutus (Hundred-pace snake) (Agkistrodon acutus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Viperidae; Crotalinae; Deinagkistrodon.
OX NCBI_TaxID=36307;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RX PubMed=10669165;
RA Chen Y.L., Tsai K.W., Chang T., Hong T.M., Tsai I.H.;
RT "Glycoprotein Ib-binding protein from the venom of Deinagkistrodon acutus
RT -- cDNA sequence, functional characterization, and three-dimensional
RT modeling.";
RL Thromb. Haemost. 83:119-126(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RA Yu H., Xiang K., Wang Y., Liu J.;
RT "A chain of antithrombin A from Deinagkistrodon acutus.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 24-32, AND FUNCTION.
RC TISSUE=Venom;
RX PubMed=15777951; DOI=10.1016/j.toxicon.2004.11.017;
RA Xu G., Ulrichts H., Vauterin S., De Meyer S.F., Deckmyn H., Teng M.,
RA Niu L.;
RT "How does agkicetin-C bind on platelet glycoprotein Ibalpha and achieve its
RT platelet effects?";
RL Toxicon 45:561-570(2005).
RN [4]
RP CRYSTALLIZATION.
RX PubMed=16508096; DOI=10.1107/s1744309104027241;
RA Xu G., Huang Q., Teng M., Liu P., Dong Y., Niu L.;
RT "Crystallization and preliminary X-ray crystallographic analysis of
RT agkicetin-C from Deinagkistrodon acutus venom.";
RL Acta Crystallogr. F 61:75-78(2005).
CC -!- FUNCTION: Is a potent glycoprotein Ibalpha (GP1BA) antagonist.
CC Concentration-dependently inhibits botrocetin-, ristocetin- and low
CC dose thrombin-induced platelet aggregation. Inhibits platelet adhesion
CC only through inhibiting the vWF interaction with GP1BA, but has minimal
CC effect on other platelet receptors, such as alpha-IIb/beta-3
CC (ITGA2B/ITGB3) or alpha-2/beta-1 (ITGA2/ITGB1). Causes an instant
CC severe thrombocytopenia in rats and is not lethal to mice.
CC {ECO:0000269|PubMed:15777951}.
CC -!- SUBUNIT: Heterodimer of subunits alpha and beta; disulfide-linked.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC -!- MISCELLANEOUS: Lacks hemorrhagic activity in rabbits. Has no inhibitory
CC effect on the coagulation cascade of humans. Does not agglutinate human
CC erythrocytes of four groups (AC, BC, OC, ABK) and rabbit erythrocytes
CC as well, which indicates it is not really a lectin (PubMed:15777951).
CC {ECO:0000305|PubMed:15777951}.
CC -!- SIMILARITY: Belongs to the snaclec family. {ECO:0000305}.
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DR EMBL; AF102901; AAG42040.1; -; mRNA.
DR EMBL; AY091762; AAM22790.1; -; mRNA.
DR AlphaFoldDB; Q9DEA2; -.
DR SMR; Q9DEA2; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR Gene3D; 3.10.100.10; -; 1.
DR InterPro; IPR001304; C-type_lectin-like.
DR InterPro; IPR016186; C-type_lectin-like/link_sf.
DR InterPro; IPR018378; C-type_lectin_CS.
DR InterPro; IPR016187; CTDL_fold.
DR Pfam; PF00059; Lectin_C; 1.
DR SMART; SM00034; CLECT; 1.
DR SUPFAM; SSF56436; SSF56436; 1.
DR PROSITE; PS00615; C_TYPE_LECTIN_1; 1.
DR PROSITE; PS50041; C_TYPE_LECTIN_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Hemostasis impairing toxin;
KW Platelet aggregation inhibiting toxin; Secreted; Signal; Toxin.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:15777951"
FT CHAIN 24..155
FT /note="Snaclec agkicetin-C subunit alpha"
FT /id="PRO_5000055286"
FT DOMAIN 32..150
FT /note="C-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 25..36
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 53..149
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 101
FT /note="Interchain (with C-98 in subunit beta)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 124..141
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT CONFLICT 31
FT /note="S -> C (in Ref. 2; AAM22790)"
FT /evidence="ECO:0000305"
FT CONFLICT 69
FT /note="R -> G (in Ref. 2; AAM22790)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 155 AA; 17799 MW; 92B40281A797AACD CRC64;
MGRFIFVSFG LLVVFLSLSG TAADCLPGWS SYIRFCYQPF KLLKTWEDAE RFCTEQANGG
HLVSFESARE ADFVAGVLSE NIKIKPYVWI GLRVQNEGQQ CSSKWSDSSK VSYENLVEPF
SKKCFVLKKD TGFRTWENVY CGLKHVFMCK YLKPR
