SLCA_DROME
ID SLCA_DROME Reviewed; 28 AA.
AC C0HJH4; Q8SYY2; X2JEE2;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 36.
DE RecName: Full=Sarcolamban A {ECO:0000312|FlyBase:FBgn0266491};
GN Name=SclA {ECO:0000312|FlyBase:FBgn0266491}; ORFNames=CG45090;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL48870.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL48870.1};
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S.;
RL Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP IDENTIFICATION, FUNCTION, INTERACTION WITH SERCA, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=23970561; DOI=10.1126/science.1238802;
RA Magny E.G., Pueyo J.I., Pearl F.M., Cespedes M.A., Niven J.E., Bishop S.A.,
RA Couso J.P.;
RT "Conserved regulation of cardiac calcium uptake by peptides encoded in
RT small open reading frames.";
RL Science 341:1116-1120(2013).
CC -!- FUNCTION: Plays an essential role in the regulation of calcium
CC transport at the sarcoplasmic reticulum (SR), which is secondarily
CC required for regular muscle contraction. {ECO:0000269|PubMed:23970561}.
CC -!- SUBUNIT: Interacts with SERCA. {ECO:0000269|PubMed:23970561}.
CC -!- SUBCELLULAR LOCATION: Sarcoplasmic reticulum membrane
CC {ECO:0000269|PubMed:23970561}; Single-pass membrane protein
CC {ECO:0000269|PubMed:23970561}. Cell membrane, sarcolemma, T-tubule
CC {ECO:0000269|PubMed:23970561}. Note=Colocalizes with SERCA at the
CC sarcoplasmic reticulum and the diad, a structure composed of a single
CC t-tubule paired with a terminal cisterna of the sarcoplasmic reticulum.
CC {ECO:0000269|PubMed:23970561}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in embryonic and larval somatic
CC muscles and postembryonic heart. {ECO:0000269|PubMed:23970561}.
CC -!- DISRUPTION PHENOTYPE: SclA and SclB double mutants show arrhythmic
CC cardiac contractions and correspondingly, cardiac cells show irregular
CC action potentials (APs), involving double and occasionally failed APs.
CC Calcium transients in these mutants show higher amplitudes and steeper
CC decay than those in wild type flies. {ECO:0000269|PubMed:23970561}.
CC -!- MISCELLANEOUS: This protein is produced by a bicistronic gene which
CC also produces the SclB protein from a non-overlapping reading frame.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL48870.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};
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DR EMBL; AE014134; AHN54508.1; -; Genomic_DNA.
DR EMBL; AE014134; AHN54509.1; -; Genomic_DNA.
DR EMBL; AE014134; AHN54510.1; -; Genomic_DNA.
DR EMBL; AY071248; AAL48870.1; ALT_SEQ; mRNA.
DR RefSeq; NP_001285994.1; NM_001299065.1.
DR RefSeq; NP_001285995.1; NM_001299066.1.
DR RefSeq; NP_001285996.1; NM_001299067.1.
DR AlphaFoldDB; C0HJH4; -.
DR BioGRID; 2594454; 2.
DR DIP; DIP-61732N; -.
DR IntAct; C0HJH4; 1.
DR EnsemblMetazoa; FBtr0344543; FBpp0310895; FBgn0266491.
DR EnsemblMetazoa; FBtr0344544; FBpp0310896; FBgn0266491.
DR EnsemblMetazoa; FBtr0344545; FBpp0310897; FBgn0266491.
DR GeneID; 19835657; -.
DR KEGG; dme:Dmel_CG45090; -.
DR CTD; 19835657; -.
DR FlyBase; FBgn0266491; SclA.
DR VEuPathDB; VectorBase:FBgn0266491; -.
DR HOGENOM; CLU_221518_0_0_1; -.
DR BioGRID-ORCS; 19835657; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 19835657; -.
DR PRO; PR:C0HJH4; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0266491; Expressed in larva and 17 other tissues.
DR Genevisible; C0HJH4; DM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0033017; C:sarcoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030315; C:T-tubule; IDA:FlyBase.
DR GO; GO:0055117; P:regulation of cardiac muscle contraction; IMP:FlyBase.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Sarcoplasmic reticulum;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..28
FT /note="Sarcolamban A"
FT /id="PRO_0000426725"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 28 AA; 3254 MW; 1CC325CA750F5685 CRC64;
MSEARNLFTT FGILAILLFF LYLIYAVL
