SLD3_SCHPO
ID SLD3_SCHPO Reviewed; 699 AA.
AC Q09761;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 22-FEB-2012, sequence version 3.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=DNA replication regulator sld3;
DE AltName: Full=Meiotically up-regulated gene 175 protein;
GN Name=sld3; Synonyms=mug175; ORFNames=SPAC24H6.06;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP FUNCTION, INTERACTION WITH CDC45; MCM6 AND MCM7, AND PHOSPHORYLATION.
RX PubMed=12006645; DOI=10.1091/mbc.02-01-0006;
RA Nakajima R., Masukata H.;
RT "SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in
RT DNA replication in fission yeast.";
RL Mol. Biol. Cell 13:1462-1472(2002).
RN [4]
RP FUNCTION IN MEIOSIS.
RX PubMed=16303567; DOI=10.1016/j.cub.2005.10.038;
RA Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L.,
RA Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P.,
RA Smith G.R., Moreno S.;
RT "A large-scale screen in S. pombe identifies seven novel genes required for
RT critical meiotic events.";
RL Curr. Biol. 15:2056-2062(2005).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Required for loading and maintenance of stable association of
CC cdc45 with chromatin during initiation and elongation of DNA
CC replication. Also involved in temporal regulation of origin firing.
CC Plays a role in meiosis. {ECO:0000269|PubMed:12006645,
CC ECO:0000269|PubMed:16303567}.
CC -!- SUBUNIT: Interacts with cdc45 and minichromosome maintenance proteins
CC mcm6 and mcm7. {ECO:0000269|PubMed:12006645}.
CC -!- INTERACTION:
CC Q09761; O74113: sna41; NbExp=2; IntAct=EBI-1559416, EBI-1559427;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16823372}.
CC -!- PTM: Phosphorylated. {ECO:0000269|PubMed:12006645}.
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DR EMBL; CU329670; CAA90850.3; -; Genomic_DNA.
DR PIR; S62408; S62408.
DR PIR; T38362; T38362.
DR RefSeq; NP_592946.2; NM_001018347.2.
DR PDB; 6HM3; X-ray; 1.77 A; B=629-656.
DR PDBsum; 6HM3; -.
DR AlphaFoldDB; Q09761; -.
DR SMR; Q09761; -.
DR BioGRID; 279082; 22.
DR IntAct; Q09761; 2.
DR STRING; 4896.SPAC24H6.06.1; -.
DR iPTMnet; Q09761; -.
DR PaxDb; Q09761; -.
DR EnsemblFungi; SPAC24H6.06.1; SPAC24H6.06.1:pep; SPAC24H6.06.
DR GeneID; 2542628; -.
DR KEGG; spo:SPAC24H6.06; -.
DR PomBase; SPAC24H6.06; sld3.
DR VEuPathDB; FungiDB:SPAC24H6.06; -.
DR eggNOG; ENOG502S01X; Eukaryota.
DR HOGENOM; CLU_403403_0_0_1; -.
DR InParanoid; Q09761; -.
DR OMA; DGLCIRR; -.
DR PRO; PR:Q09761; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000785; C:chromatin; IDA:PomBase.
DR GO; GO:0031261; C:DNA replication preinitiation complex; IBA:GO_Central.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:1902975; P:mitotic DNA replication initiation; IMP:PomBase.
DR InterPro; IPR013948; DNA_replication_reg_Sld3_C.
DR InterPro; IPR042511; Sld3.
DR PANTHER; PTHR28067; PTHR28067; 1.
DR Pfam; PF08639; SLD3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; DNA replication; Meiosis; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..699
FT /note="DNA replication regulator sld3"
FT /id="PRO_0000071953"
FT REGION 459..505
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 517..568
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 590..610
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 482..505
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 533..549
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 553..568
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 631..633
FT /evidence="ECO:0007829|PDB:6HM3"
SQ SEQUENCE 699 AA; 79815 MW; 398512B0ABD4B66E CRC64;
MNNDHASKKS FCIKAPSNWE KSYLEVWPLV TVPRQCICLR WCISKEYHEF TCSSLQFIVI
RPAGTSVLLG RVKSSKANQL VGIEHVEGSR YALIFLSEKL DFKSLKVIAN HQLTKSSKSL
SNVSNKPLGD QLFRSNSLMS PSLLKKELHR IQSDASQANE RESQAPHSFV THDLISSSKD
GNSLTHEFAN DSVTEMVQDY TPSCSRDVKS LLDHLYNSYF YQLLMTKTPV VFYVKQMVGK
TRQLAVEVHN HVEEKALVDE LLKFLDNLKS VDDRKSRLLQ CFESHLNYKA WHLEFENEAH
QYEIKGYRLW LQNILNRENC QITKLDFERE FSQLKLKEYE IRVLLYFEIL YLFLKWDPEY
ARRRANDNSL LDSRDSGKRK SRKKNAKTLN PFETAQLKLE FTFDGLCIRR TIEQNATERS
EDLLLKFCKE TIVPYYSSKF PRITRNLLEK CNGLDLLPER SHKHRHSAPP RSKLISSKSE
AGRALPGNTS GASISNTSSP HSEASISKDY EILKRRRSNS GVHSLTRSDS SFNGFERDTR
RRSSDIARIK NREINLPSSS LSKQRNSMHD ISTNFPRRNL SFTEKLTMAS LQGQSEESVQ
PKTTSSLSRS KTLSILEGSV SKRSEPSMDS ILVQATPRKS SSVITELPDT PIKMNSLDKA
SACTVENHIV TESPAHKSNK AQLFVCVPTT PVKKKSASP
