SLD3_YEAST
ID SLD3_YEAST Reviewed; 668 AA.
AC P53135; D6VU34;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=DNA replication regulator SLD3;
GN Name=SLD3; OrderedLocusNames=YGL113W; ORFNames=G2980;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 492-668.
RX PubMed=9046090;
RX DOI=10.1002/(sici)1097-0061(199701)13:1<85::aid-yea53>3.0.co;2-e;
RA Paoluzi S., Minenkova O., Castagnoli L.;
RT "The genes encoding the transcription factor yTAFII60, the G4p1 protein and
RT a putative glucose transporter are contained in a 12.3 kb DNA fragment on
RT the left arm of Saccharomyces cerevisiae chromosome VII.";
RL Yeast 13:85-91(1997).
RN [4]
RP FUNCTION, AND INTERACTION WITH CDC45.
RX PubMed=11296242; DOI=10.1093/emboj/20.8.2097;
RA Kamimura Y., Tak Y.-S., Sugino A., Araki H.;
RT "Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA
RT replication in Saccharomyces cerevisiae.";
RL EMBO J. 20:2097-2107(2001).
RN [5]
RP FUNCTION.
RX PubMed=12730134; DOI=10.1101/gad.1065903;
RA Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H.;
RT "GINS, a novel multiprotein complex required for chromosomal DNA
RT replication in budding yeast.";
RL Genes Dev. 17:1153-1165(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP FUNCTION, AND INTERACTION WITH SLD7.
RX PubMed=21487389; DOI=10.1038/emboj.2011.115;
RA Tanaka T., Umemori T., Endo S., Muramatsu S., Kanemaki M., Kamimura Y.,
RA Obuse C., Araki H.;
RT "Sld7, an Sld3-associated protein required for efficient chromosomal DNA
RT replication in budding yeast.";
RL EMBO J. 30:2019-2030(2011).
CC -!- FUNCTION: Required for loading and maintenance of stable association of
CC CDC45 with chromatin during initiation and elongation of DNA
CC replication. Also involved in temporal regulation of origin firing.
CC Required for the association of PSF1 with origins.
CC {ECO:0000269|PubMed:11296242, ECO:0000269|PubMed:12730134,
CC ECO:0000269|PubMed:21487389}.
CC -!- SUBUNIT: Interacts with CDC45 and SLD7. {ECO:0000269|PubMed:11296242,
CC ECO:0000269|PubMed:21487389}.
CC -!- INTERACTION:
CC P53135; Q08032: CDC45; NbExp=13; IntAct=EBI-23925, EBI-4292;
CC P53135; P47027: DPB11; NbExp=9; IntAct=EBI-23925, EBI-25984;
CC P53135; P22216: RAD53; NbExp=3; IntAct=EBI-23925, EBI-17843;
CC P53135; Q08457: SLD7; NbExp=9; IntAct=EBI-23925, EBI-36267;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- MISCELLANEOUS: Present with 125 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z72635; CAA96821.1; -; Genomic_DNA.
DR EMBL; Z72634; CAA96820.1; -; Genomic_DNA.
DR EMBL; X97644; CAA66239.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA07995.1; -; Genomic_DNA.
DR PIR; S64123; S64123.
DR RefSeq; NP_011402.3; NM_001180978.3.
DR PDB; 3WI3; X-ray; 2.40 A; A/B/C=148-430.
DR PDBsum; 3WI3; -.
DR AlphaFoldDB; P53135; -.
DR SMR; P53135; -.
DR BioGRID; 33138; 253.
DR ComplexPortal; CPX-1188; DPB11-SLD3-SLD2 DNA replication complex.
DR DIP; DIP-5481N; -.
DR IntAct; P53135; 9.
DR MINT; P53135; -.
DR STRING; 4932.YGL113W; -.
DR iPTMnet; P53135; -.
DR MaxQB; P53135; -.
DR PaxDb; P53135; -.
DR PRIDE; P53135; -.
DR EnsemblFungi; YGL113W_mRNA; YGL113W; YGL113W.
DR GeneID; 852765; -.
DR KEGG; sce:YGL113W; -.
DR SGD; S000003081; SLD3.
DR VEuPathDB; FungiDB:YGL113W; -.
DR eggNOG; ENOG502RE09; Eukaryota.
DR HOGENOM; CLU_409479_0_0_1; -.
DR InParanoid; P53135; -.
DR OMA; SLDYCEQ; -.
DR BioCyc; YEAST:G3O-30611-MON; -.
DR ChiTaRS; SLD3; yeast.
DR PRO; PR:P53135; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53135; protein.
DR GO; GO:0000785; C:chromatin; IDA:SGD.
DR GO; GO:0000775; C:chromosome, centromeric region; IDA:SGD.
DR GO; GO:0031261; C:DNA replication preinitiation complex; IPI:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0003682; F:chromatin binding; IDA:SGD.
DR GO; GO:0006270; P:DNA replication initiation; IMP:SGD.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IMP:SGD.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IMP:SGD.
DR GO; GO:0033314; P:mitotic DNA replication checkpoint signaling; IC:ComplexPortal.
DR GO; GO:1903466; P:regulation of mitotic DNA replication initiation; IC:ComplexPortal.
DR InterPro; IPR013948; DNA_replication_reg_Sld3_C.
DR InterPro; IPR042511; Sld3.
DR InterPro; IPR041393; Sld3_N.
DR PANTHER; PTHR28067; PTHR28067; 1.
DR Pfam; PF08639; SLD3; 1.
DR Pfam; PF18523; Sld3_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; DNA replication; Nucleus; Reference proteome.
FT CHAIN 1..668
FT /note="DNA replication regulator SLD3"
FT /id="PRO_0000071954"
FT REGION 429..450
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 621..668
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 431..450
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 637..651
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 652..668
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 157..170
FT /evidence="ECO:0007829|PDB:3WI3"
FT HELIX 176..181
FT /evidence="ECO:0007829|PDB:3WI3"
FT HELIX 183..195
FT /evidence="ECO:0007829|PDB:3WI3"
FT TURN 197..199
FT /evidence="ECO:0007829|PDB:3WI3"
FT HELIX 200..209
FT /evidence="ECO:0007829|PDB:3WI3"
FT HELIX 215..223
FT /evidence="ECO:0007829|PDB:3WI3"
FT HELIX 226..228
FT /evidence="ECO:0007829|PDB:3WI3"
FT HELIX 234..247
FT /evidence="ECO:0007829|PDB:3WI3"
FT HELIX 258..284
FT /evidence="ECO:0007829|PDB:3WI3"
FT HELIX 288..291
FT /evidence="ECO:0007829|PDB:3WI3"
FT HELIX 339..358
FT /evidence="ECO:0007829|PDB:3WI3"
FT HELIX 373..380
FT /evidence="ECO:0007829|PDB:3WI3"
FT HELIX 390..396
FT /evidence="ECO:0007829|PDB:3WI3"
FT HELIX 398..402
FT /evidence="ECO:0007829|PDB:3WI3"
FT STRAND 403..406
FT /evidence="ECO:0007829|PDB:3WI3"
FT HELIX 407..417
FT /evidence="ECO:0007829|PDB:3WI3"
SQ SEQUENCE 668 AA; 77298 MW; 6E59D0DB3C082C28 CRC64;
METWEVIASV KEATKGLDLS LDHPLIIKSE DVPSNILQLL QQKNRRQLKH ICMKSRKEYF
LLEEYGPGFW VKWPYNYFNG YSLPERRTEV VTTVERERAK RETLKTWDEL KFKELLHLWS
EEPKGSCKLE KDKDLKLDMN PPDMKGESKI NDYYSDPKEY IESKYYDALF SIHTPLAYFV
KSNLVRLKNT CRTKYGSDSY KIAYQAMLQK FLLSIVQFKD RHDNRLLLEP FSSPIADEKR
KNCLTKFVIQ DENKNSSTIA DLCVVLKSRE IKLQILLLLE IIGLNDLDWN FRDFEKKYKL
KLKKRSLNLT KKGLVRRRSK KKTSEKDKGI ERITTSLDYC EQLDLYLDRA CILDILLSSE
TPNPDAIEAS NGTIQEHKKN ILDKSKEASL VGFINYVLIP YFNKKVPHAV EFIIQKLKGP
SMRPKRALKK VNDSTNVSSP NTVETYNRLS TSQRASRSSI INSVPSSPAL RRVDANLFSR
KSIASPTPEL LNSRTNSNLN EFLESETRSL KRPSQLGRTK SDLTMNHLQK RQFSVSDLST
TRVPNSSTIT LKTPFSHSTI NAYKTMNNSF RRVGKRKDIN ETIRLHERVD SEENVQVQAT
PAVKKRTVTP NKKAQLQSII ESPLNFKDDD THEGRKNTSN ITSTPTNKPP ENSSKRRVRR
RLFAPEST
